{"data":{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35047008", "kind": "science-record", "revision": 4, "content_hash": "sha256:ef5cdd811c449641a42f34384960b061c215082d7224ef8a6b52a216076dd6a4", "created_at": "2026-11-01T17:36:46Z", "updated_at": "2026-11-01T22:05:37Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification and Verification of Five Potential Biomarkers Related to Skin and Thermal Injury Using Weighted Gene Co-Expression Network Analysis.", "doi": "10.3389/fgene.2021.781589", "pmid": "35047008", "authors": ["Ronghua Yang", "Zhengguang Wang", "Jiehua Li", "Xiaobing Pi", "Xiaoxiang Wang", "Yang Xu", "Yan Shi", "Sitong Zhou"], "journal": "Frontiers in genetics", "year": 2021, "abstract": "Background: Burn injury is a life-threatening disease that does not have ideal biomarkers. Therefore, this study first applied weighted gene co-expression network analysis (WGCNA) and differentially expressed gene (DEG) screening methods to identify pivotal genes and diagnostic biomarkers associated with the skin burn process. Methods: After obtaining transcriptomic datasets of burn patient skin and normal skin from Gene Expression Omnibus (GEO) and performing differential analysis and functional enrichment, WGCNA was used to identify hub gene modules associated with burn skin processes in the burn patient peripheral blood sample dataset and determine the correlation between modules and clinical features. Enrichment analysis was performed to identify the functions and pathways of key module genes. Differential analysis, WGCNA, protein-protein interaction analysis, and enrichment analysis were utilized to screen for hub genes. Hub genes were validated in two other GEO datasets, tested by immunohistochemistry for hub gene expression in burn patients, and receiver operating characteristic curve analysis was performed. Finally, we constructed the specific drug activity, transcription factors, and microRNA regulatory network of the five hub genes. Results: A total of 1,373 DEGs in GSE8056 were obtained, and the top 5 upregulated genes were S100A12, CXCL8, CXCL5, MMP3, and MMP1, whereas the top 5 downregulated genes were SCGB1D2, SCGB2A2, DCD, TSPAN8, and KRT25. DEGs were significantly enriched in the immunity, epidermal development, and skin development processes. In WGCNA, the yellow module was identified as the most closely associated module with tissue damage during the burn process, and the five hub genes (ANXA3, MCEMP1, MMP9, S100A12, and TCN1) were identified as the key genes for burn injury status, which consistently showed high expression in burn patient blood samples in the GSE37069 and GSE13902 datasets. Furthermore, we verified using immunohistochemistry that these five novel hub genes were also significantly elevated in burn patient skin. In addition, MCEMP1, MMP9, and S100A12 showed perfect diagnostic performance in the receiver operating characteristic analysis. Conclusion: In conclusion, we analyzed the changes in genetic processes in the skin during burns and used them to identify five potential novel diagnostic markers in blood samples from burn patients, which are important for burn patient diagnosis. In particular, MCEMP1, MMP9, and S100A12 are three key blood biomarkers that can be used to identify skin damage in burn patients.", "claims": [{"id": "claim-method", "statement": "Weighted gene co-expression network analysis (WGCNA) (Langfelder and Horvath, 2008), a novel systems biology method, assists in the construction of free-scale gene co-expression networks and the detection of gene modules and hub genes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35047008", "locator": "sections[1] \"Introduction\" paragraph 4", "quote": "Weighted gene co-expression network analysis (WGCNA) (Langfelder and Horvath, 2008), a novel systems biology method, assists in the construction of free-scale gene co-expression networks and the detection of gene modules and hub genes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35047008", "locator": "sections[24] \"Data Availability Statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We obtained 5 modules except for the gray module, in which the yellow consensus module was the most relevant module with burn traits (correlation value = 0.89; significance level p < 0.05).", "kind": "quantitative", "target": {"value": 0.05, "unit": "p-value", "tolerance": null, "source": "We obtained 5 modules except for the gray module, in which the yellow consensus module was the most relevant module with burn traits (correlation value = 0.89; significance level p < 0.05)."}, "evidence": [{"asset": "source:pmid:35047008", "locator": "sections[16] \"WGCNA and Identification of Hub Genes\" paragraph 2", "quote": "We obtained 5 modules except for the gray module, in which the yellow consensus module was the most relevant module with burn traits (correlation value = 0.89; significance level p < 0.05)."}, {"asset": "source:pmid:35047008", "locator": "sections[16] \"WGCNA and Identification of Hub Genes\" paragraph 2", "quote": "Furthermore, 30 hub genes were selected in yellow co-expression modules with gene significance >0.8 and module membership >0.8."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "We obtained 5 modules except for the gray module, in which the yellow consensus module was the most relevant module with burn traits (correlation value = 0.89; significance level p < 0.05).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35047008"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE123568"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE123568"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35047008", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fgene.2021.781589", "sha256": "sha256:bc35dab1aa13c4433ce2a0bfa5077e891b0a3a2b144eddd049bc782a67c4d0e8", "bytes": 40649, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fgene.2021.781589", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8762241", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE123568", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE123568", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE123568", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM3507251", "GSM3507252", "GSM3507253", "GSM3507254", "GSM3507255", "GSM3507256", "GSM3507257", "GSM3507258", "GSM3507259", "GSM3507260", "GSM3507261", "GSM3507262", "GSM3507263", "GSM3507264", "GSM3507265", "GSM3507266", "GSM3507267", "GSM3507268", "GSM3507269", "GSM3507270", "GSM3507271", "GSM3507272", "GSM3507273", "GSM3507274", "GSM3507275", "GSM3507276", "GSM3507277", "GSM3507278", "GSM3507279", "GSM3507280", "GSM3507281", "GSM3507282", "GSM3507283", "GSM3507284", "GSM3507285", "GSM3507286", "GSM3507287", "GSM3507288", "GSM3507289", "GSM3507290"], "sample_count": 40, "group_sizes": null, "group_sizes_basis": null, "platform": "15207", "taxon": "Homo sapiens", "title": "Identification of Potential Biomarkers for Improving the Precision of Early Detection of Steroid-induced Osteonecrosis of the Femoral Head", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE13902", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13902", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE13902", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM350095", "GSM350102"], "sample_count": 2, "group_sizes": null, "group_sizes_basis": null, "platform": "3154", "taxon": "Escherichia coli", "title": "BW25113 fhlA/pCA24N-FhlA133 vs fhlA/pCA24N-FhlA in modified-complex 20 mM formate at 37ºC", "resolution_checked_at": "2026-11-01T17:30:42Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE139028", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE139028", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE139028", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM4127070", "GSM4127071", "GSM4127072", "GSM4127073", "GSM4127074", "GSM4127075", "GSM4127076", "GSM4127077", "GSM4127078"], "sample_count": 9, "group_sizes": null, "group_sizes_basis": null, "platform": "15433", "taxon": "Homo sapiens", "title": "RNA-seq analysis on tissues derived from normal skin and burn eschar", "resolution_checked_at": "2026-11-01T17:30:42Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE19743", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE19743", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE19743", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM493655", "GSM493656", "GSM493657", "GSM493658", "GSM493659", "GSM493660", "GSM493661", "GSM493662", "GSM493663", "GSM493664", "GSM493665", "GSM493666", "GSM493667", "GSM493668", "GSM493669", "GSM493670", "GSM493671", "GSM493672", "GSM493673", "GSM493674", "GSM493675", "GSM493676", "GSM493677", "GSM493678", "GSM493679", "GSM493680", "GSM493681", "GSM493682", "GSM493683", "GSM493684", "GSM493685", "GSM493686", "GSM493687", "GSM493688", "GSM493689", "GSM493690", "GSM493691", "GSM493692", "GSM493693", "GSM493694", "GSM493695", "GSM493696", "GSM493697", "GSM493698", "GSM493699", "GSM493700", "GSM493701", "GSM493702", "GSM493703", "GSM493704", "GSM493705", "GSM493706", "GSM493707", "GSM493708", "GSM493709", "GSM493710", "GSM493711", "GSM493712", "GSM493713", "GSM493714", "GSM493715", "GSM493716", "GSM493717", "GSM493718", "GSM493719", "GSM493720", "GSM493721", "GSM493722", "GSM493723", "GSM493724", "GSM493725", "GSM493726", "GSM493727", "GSM493728", "GSM493729", "GSM493730", "GSM493731", "GSM493732", "GSM493733", "GSM493734", "GSM493735", "GSM493736", "GSM493737", "GSM493738", "GSM493739", "GSM493740", "GSM493741", "GSM493742", "GSM493743", "GSM493744", "GSM493745", "GSM493746", "GSM493747", "GSM493748", "GSM493749", "GSM493750", "GSM493751", "GSM493752", "GSM493753", "GSM493754", "GSM493755", "GSM493756", "GSM493757", "GSM493758", "GSM493759", "GSM493760", "GSM493761", "GSM493762", "GSM493763", "GSM493764", "GSM493765", "GSM493766", "GSM493767", "GSM493768", "GSM493769", "GSM493770", "GSM493771", "GSM493772", "GSM493773", "GSM493774", "GSM493775", "GSM493776", "GSM493777", "GSM493778", "GSM493779", "GSM493780", "GSM493781", "GSM493782", "GSM493783", "GSM493784", "GSM493785", "GSM493786", "GSM493787", "GSM493788", "GSM493789", "GSM493790", "GSM493791", "GSM493792", "GSM493793", "GSM493794", "GSM493795", "GSM493796", "GSM493797", "GSM493798", "GSM493799", "GSM493800", "GSM493801", "GSM493802", "GSM493803", "GSM493804", "GSM493805", "GSM493806", "GSM493807", "GSM493808", "GSM493809", "GSM493810", "GSM493811", "GSM493812", "GSM493813", "GSM493814", "GSM493815", "GSM493816", "GSM493817", "GSM493818", "GSM493819", "GSM493820", "GSM493821", "GSM493822", "GSM493823", "GSM493824", "GSM493825", "GSM493826", "GSM493827", "GSM493828", "GSM493829", "GSM493830", "GSM493831"], "sample_count": 177, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "A large-scale clinical study of gene expression response to severe burn injury", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE37069", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE37069", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE37069", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM909644", "GSM909645", "GSM909646", "GSM909647", "GSM909648", "GSM909649", "GSM909650", "GSM909651", "GSM909652", "GSM909653", "GSM909654", "GSM909655", "GSM909656", "GSM909657", "GSM909658", "GSM909659", "GSM909660", "GSM909661", "GSM909662", "GSM909663", "GSM909664", "GSM909665", "GSM909666", "GSM909667", "GSM909668", "GSM909669", "GSM909670", "GSM909671", "GSM909672", "GSM909673", "GSM909674", "GSM909675", "GSM909676", "GSM909677", "GSM909678", "GSM909679", "GSM909680", "GSM909681", "GSM909682", "GSM909683", "GSM909684", "GSM909685", "GSM909686", "GSM909687", "GSM909688", "GSM909689", "GSM909690", "GSM909691", "GSM909692", "GSM909693", "GSM909694", "GSM909695", "GSM909696", "GSM909697", "GSM909698", "GSM909699", "GSM909700", "GSM909701", "GSM909702", "GSM909703", "GSM909704", "GSM909705", "GSM909706", "GSM909707", "GSM909708", "GSM909709", "GSM909710", "GSM909711", "GSM909712", "GSM909713", "GSM909714", "GSM909715", "GSM909716", "GSM909717", "GSM909718", "GSM909719", "GSM909720", "GSM909721", "GSM909722", "GSM909723", "GSM909724", "GSM909725", "GSM909726", "GSM909727", "GSM909728", "GSM909729", "GSM909730", "GSM909731", "GSM909732", "GSM909733", "GSM909734", "GSM909735", "GSM909736", "GSM909737", "GSM909738", "GSM909739", "GSM909740", "GSM909741", "GSM909742", "GSM909743", "GSM909744", "GSM909745", "GSM909746", "GSM909747", "GSM909748", "GSM909749", "GSM909750", "GSM909751", "GSM909752", "GSM909753", "GSM909754", "GSM909755", "GSM909756", "GSM909757", "GSM909758", "GSM909759", "GSM909760", "GSM909761", "GSM909762", "GSM909763", "GSM909764", "GSM909765", "GSM909766", "GSM909767", "GSM909768", "GSM909769", "GSM909770", "GSM909771", "GSM909772", "GSM909773", "GSM909774", "GSM909775", "GSM909776", "GSM909777", "GSM909778", "GSM909779", "GSM909780", "GSM909781", "GSM909782", "GSM909783", "GSM909784", "GSM909785", "GSM909786", "GSM909787", "GSM909788", "GSM909789", "GSM909790", "GSM909791", "GSM909792", "GSM909793", "GSM909794", "GSM909795", "GSM909796", "GSM909797", "GSM909798", "GSM909799", "GSM909800", "GSM909801", "GSM909802", "GSM909803", "GSM909804", "GSM909805", "GSM909806", "GSM909807", "GSM909808", "GSM909809", "GSM909810", "GSM909811", "GSM909812", "GSM909813", "GSM909814", "GSM909815", "GSM909816", "GSM909817", "GSM909818", "GSM909819", "GSM909820", "GSM909821", "GSM909822", "GSM909823", "GSM909824", "GSM909825", "GSM909826", "GSM909827", "GSM909828", "GSM909829", "GSM909830", "GSM909831", "GSM909832", "GSM909833", "GSM909834", "GSM909835", "GSM909836", "GSM909837", "GSM909838", "GSM909839", "GSM909840", "GSM909841", "GSM909842", "GSM909843", "GSM909844", "GSM909845", "GSM909846", "GSM909847", "GSM909848", "GSM909849", "GSM909850", "GSM909851", "GSM909852", "GSM909853", "GSM909854", "GSM909855", "GSM909856", "GSM909857", "GSM909858", "GSM909859", "GSM909860", "GSM909861", "GSM909862", "GSM909863", "GSM909864", "GSM909865", "GSM909866", "GSM909867", "GSM909868", "GSM909869", "GSM909870", "GSM909871", "GSM909872", "GSM909873", "GSM909874", "GSM909875", "GSM909876", "GSM909877", "GSM909878", "GSM909879", "GSM909880", "GSM909881", "GSM909882", "GSM909883", "GSM909884", "GSM909885", "GSM909886", "GSM909887", "GSM909888", "GSM909889", "GSM909890", "GSM909891", "GSM909892", "GSM909893", "GSM909894", "GSM909895", "GSM909896", "GSM909897", "GSM909898", "GSM909899", "GSM909900", "GSM909901", "GSM909902", "GSM909903", "GSM909904", "GSM909905", "GSM909906", "GSM909907", "GSM909908", "GSM909909", "GSM909910", "GSM909911", "GSM909912", "GSM909913", "GSM909914", "GSM909915", "GSM909916", "GSM909917", "GSM909918", "GSM909919", "GSM909920", "GSM909921", "GSM909922", "GSM909923", "GSM909924", "GSM909925", "GSM909926", "GSM909927", "GSM909928", "GSM909929", "GSM909930", "GSM909931", "GSM909932", "GSM909933", "GSM909934", "GSM909935", "GSM909936", "GSM909937", "GSM909938", "GSM909939", "GSM909940", "GSM909941", "GSM909942", "GSM909943", "GSM909944", "GSM909945", "GSM909946", "GSM909947", "GSM909948", "GSM909949", "GSM909950", "GSM909951", "GSM909952", "GSM909953", "GSM909954", "GSM909955", "GSM909956", "GSM909957", "GSM909958", "GSM909959", "GSM909960", "GSM909961", "GSM909962", "GSM909963", "GSM909964", "GSM909965", "GSM909966", "GSM909967", "GSM909968", "GSM909969", "GSM909970", "GSM909971", "GSM909972", "GSM909973", "GSM909974", "GSM909975", "GSM909976", "GSM909977", "GSM909978", "GSM909979", "GSM909980", "GSM909981", "GSM909982", "GSM909983", "GSM909984", "GSM909985", "GSM909986", "GSM909987", "GSM909988", "GSM909989", "GSM909990", "GSM909991", "GSM909992", "GSM909993", "GSM909994", "GSM909995", "GSM909996", "GSM909997", "GSM909998", "GSM909999", "GSM910000", "GSM910001", "GSM910002", "GSM910003", "GSM910004", "GSM910005", "GSM910006", "GSM910007", "GSM910008", "GSM910009", "GSM910010", "GSM910011", "GSM910012", "GSM910013", "GSM910014", "GSM910015", "GSM910016", "GSM910017", "GSM910018", "GSM910019", "GSM910020", "GSM910021", "GSM910022", "GSM910023", "GSM910024", "GSM910025", "GSM910026", "GSM910027", "GSM910028", "GSM910029", "GSM910030", "GSM910031", "GSM910032", "GSM910033", "GSM910034", "GSM910035", "GSM910036", "GSM910037", "GSM910038", "GSM910039", "GSM910040", "GSM910041", "GSM910042", "GSM910043", "GSM910044", "GSM910045", "GSM910046", "GSM910047", "GSM910048", "GSM910049", "GSM910050", "GSM910051", "GSM910052", "GSM910053", "GSM910054", "GSM910055", "GSM910056", "GSM910057", "GSM910058", "GSM910059", "GSM910060", "GSM910061", "GSM910062", "GSM910063", "GSM910064", "GSM910065", "GSM910066", "GSM910067", "GSM910068", "GSM910069", "GSM910070", "GSM910071", "GSM910072", "GSM910073", "GSM910074", "GSM910075", "GSM910076", "GSM910077", "GSM910078", "GSM910079", "GSM910080", "GSM910081", "GSM910082", "GSM910083", "GSM910084", "GSM910085", "GSM910086", "GSM910087", "GSM910088", "GSM910089", "GSM910090", "GSM910091", "GSM910092", "GSM910093", "GSM910094", "GSM910095", "GSM910096", "GSM910097", "GSM910098", "GSM910099", "GSM910100", "GSM910101", "GSM910102", "GSM910103", "GSM910104", "GSM910105", "GSM910106", "GSM910107", "GSM910108", "GSM910109", "GSM910110", "GSM910111", "GSM910112", "GSM910113", "GSM910114", "GSM910115", "GSM910116", "GSM910117", "GSM910118", "GSM910119", "GSM910120", "GSM910121", "GSM910122", "GSM910123", "GSM910124", "GSM910125", "GSM910126", "GSM910127", "GSM910128", "GSM910129", "GSM910130", "GSM910131", "GSM910132", "GSM910133", "GSM910134", "GSM910135", "GSM910136", "GSM910137", "GSM910138", "GSM910139", "GSM910140", "GSM910141", "GSM910142", "GSM910143", "GSM910144", "GSM910145", "GSM910146", "GSM910147", "GSM910148", "GSM910149", "GSM910150", "GSM910151", "GSM910152", "GSM910153", "GSM910154", "GSM910155", "GSM910156", "GSM910157", "GSM910158", "GSM910159", "GSM910160", "GSM910161", "GSM910162", "GSM910163", "GSM910164", "GSM910165", "GSM910166", "GSM910167", "GSM910168", "GSM910169", "GSM910170", "GSM910171", "GSM910172", "GSM910173", "GSM910174", "GSM910175", "GSM910176", "GSM910177", "GSM910178", "GSM910179", "GSM910180", "GSM910181", "GSM910182", "GSM910183", "GSM910184", "GSM910185", "GSM910186", "GSM910187", "GSM910188", "GSM910189", "GSM910190", "GSM910191", "GSM910192", "GSM910193", "GSM910194", "GSM910195", "GSM910196", "GSM910197", "GSM910198", "GSM910199", "GSM910200", "GSM910201", "GSM910202", "GSM910203", "GSM910204", "GSM910205", "GSM910206", "GSM910207", "GSM910208", "GSM910209", "GSM910210", "GSM910211", "GSM910212", "GSM910213", "GSM910214", "GSM910215", "GSM910216", "GSM910217", "GSM910218", "GSM910219", "GSM910220", "GSM910221", "GSM910222", "GSM910223", "GSM910224", "GSM910225", "GSM910226", "GSM910227", "GSM910228", "GSM910229", "GSM910230", "GSM910231", "GSM910232", "GSM910233"], "sample_count": 590, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Gene response to major burn injuries", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE8056", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE8056", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE8056", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM198866", "GSM198867", "GSM198868", "GSM198869", "GSM198870", "GSM198871", "GSM198872", "GSM198873", "GSM198874", "GSM198875", "GSM198876", "GSM198877"], "sample_count": 12, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Gene Expression Profiles in Thermally Injured Human Skin: A Temporal Microarray Analysis", "resolution_checked_at": "2026-11-01T17:30:42Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE123568 not downloaded", "asset:data:geo:GSE123568 licence not read", "asset:data:geo:GSE13902 not downloaded", "asset:data:geo:GSE13902 licence not read", "asset:data:geo:GSE139028 not downloaded", "asset:data:geo:GSE139028 licence not read", "asset:data:geo:GSE19743 not downloaded", "asset:data:geo:GSE19743 licence not read", "asset:data:geo:GSE37069 not downloaded", "asset:data:geo:GSE37069 licence not read", "asset:data:geo:GSE8056 not downloaded", "asset:data:geo:GSE8056 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.7, "band": "medium", "quote": "protein-protein interaction"}}, {"id": "method-2", "family": "network_analysis", "specific": "pathway_enrichment", "label": "Network / pathway analysis", "dsl_operations": ["pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.8, "band": "high", "quote": "pathway enrichment"}}, {"id": "method-3", "family": "diagnostic_evaluation", "specific": "roc", "label": "Diagnostic test evaluation", "dsl_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["reference_standard_and_test"], "evidence": {"score": 0.8, "band": "high", "quote": "ROC Analysis"}}, {"id": "method-4", "family": null, "specific": null, "label": "Weighted gene co-expression network analysis (WGCNA)", "dsl_operations": [], "missing_operations": [], "expressible": null, "parameters": {}, "data_shapes": [], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Weighted gene co-expression network analysis (WGCNA) (Langfelder and Horvath, 2008), a novel systems biology method, assists in the construction of free-scale gene co-expression networks and the detection of gene modules and hub genes."}}], "targets": [{"id": "target-published-estimate", "claim_id": "claim-target-result", "operation": "build_graph", "expected_value": 0.05, "unit": "p-value", "tolerance": null, "state": "complete", "note": "The threshold the paper applied to its own result, as the paper states it. A run that reproduces the effect is judged against this threshold."}], "gaps": ["method.reader_named_unmapped"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: ppi_network."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE8056", "GSE19743", "GSE37069", "GSE139028", "GSE13902", "GSE123568"], "resolved_accessions": ["GSE8056", "GSE19743", "GSE37069", "GSE139028", "GSE13902", "GSE123568"], "statements": ["The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors."], "basis": "The paper names GSE8056, GSE19743, GSE37069, GSE139028, GSE13902, GSE123568 in NCBI GEO and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "assets": ["source:pmid:35047008", "data:geo:GSE123568"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE123568"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T17:16:08Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 11131, "completion_tokens": 2361, "cached_tokens": 0}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:35047008", "type": "paper", "label": "pmid:35047008"}, {"id": "source:pmid:35047008", "type": "manuscript", "label": "PMID 35047008 full text"}, {"id": "data:geo:GSE123568", "type": "dataset", "label": "GEO GSE123568"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:35047008", "type": "paper", "label": "pmid:35047008"}, {"id": "source:pmid:35047008", "type": "manuscript", "label": "PMID 35047008 full text"}, {"id": "data:geo:GSE123568", "type": "dataset", "label": "GEO GSE123568"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-35047008", "type": "extraction", "used": ["source:pmid:35047008"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE123568", "type": "accession_resolution", "used": ["data:geo:GSE123568"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:35047008", "parent_hash": "sha256:4195b9ac7f2a191ace4475771eaae4f4dc804081a80331065ab8aee3c4a671fe", "fork_of": null, "generation": 3}, "gaps": ["asset:data:geo:GSE123568 licence not read", "asset:data:geo:GSE123568 not downloaded", "asset:data:geo:GSE13902 licence not read", "asset:data:geo:GSE13902 not downloaded", "asset:data:geo:GSE139028 licence not read", "asset:data:geo:GSE139028 not downloaded", "asset:data:geo:GSE19743 licence not read", "asset:data:geo:GSE19743 not downloaded", "asset:data:geo:GSE37069 licence not read", "asset:data:geo:GSE37069 not downloaded", "asset:data:geo:GSE8056 licence not read", "asset:data:geo:GSE8056 not downloaded", "execution.not_run", "method.reader_named_unmapped", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["lidated in two other GEO datasets, tested by immunohistochemistry for hub gene expression in burn patients, an", "902 datasets. Furthermore, we verified using immunohistochemistry that these five novel hub genes were also si", "e Collection of Patient Tissue Specimens and IHC Staining We collected 13 burn patient skin s", "dded skin tissues were sectioned at 4 μm for IHC. Antigen retrieval was performed by incubati"], "order_basis": "paper_text_order:1105", "id": "assay:1"}, {"role": "data_generation", "definition_id": "clinical_cohort", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["y five potential novel diagnostic markers in blood samples from burn patients, which are important for burn"], "order_basis": "paper_text_order:2479", "id": "assay:2"}, {"role": "analysis", "definition_id": "network_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.7, "evidence": ["protein-protein interaction"], "order_basis": "consumes_earlier_output", "id": "assay:3"}]},"meta":{"api_version":"v1","automation_ladder_snapshot":"ba34b49490d9bfaf","automation_ladder_caveats":["Method extraction is rule-based and has no hand-judged accuracy sample. Evidence bands order textual support; method agreement intervals are null (n=0), never probabilities. Work-type validation does not validate methods.","Operation coverage describes only explicitly named methods. It does not prove a complete protocol, compatible parameters, successful compilation or re-execution. Unrecognised or ambiguous methods require review.","Data reachability is a textual accession or public-data statement. No dataset was downloaded or checked for access, completeness or licence.","Capabilities are planning states seeded from the dated equipment register. No bench ownership, completed quote or partner booking is inferred. Procurement counts are dependencies, not guaranteed replications or financial returns.","Tier and blocker counts are deterministic assessments of this corpus, not measured agreement. R3 is reserved for procurement; unresolved methods and data require human work (R4). The most restrictive axis wins, with capability, method, data as tie-breaks. Resolving one blocker can leave another at the same tier."],"engine":"corpus_engine","engine_provider":"rule_based_v1+lex99e1195c7d6f","run_id":"run-20261023T122449232913","source":"esearch","source_detail":"PubMed mirror biomed_pubmed (read-only)","run_status":"complete","imported_at":"2026-11-03T20:16:52+00:00","generated_at":"2026-11-04T01:42:49+00:00","total_papers":5529,"total_assays_tracked":29,"automatable_assay_families":6,"validated":true,"caveat_count":9,"blocking_caveats":5,"caveats":[{"code":"low_evidence_dominates","basis":["4,152 of 5,529 classifications (75.1%) matched no cue evidence and sit at the 0.05 floor.","34 of 230 data_only calls (14.8%) are in that group."],"claim":"Work type and confidence bands","gloss":"Most records were classified from their title and framing alone, with assay-cue coverage at zero.","detail":"Most classifications in this run rest on no matched evidence at all. At the evidence floor the work type comes from title framing and a fixed prior, not from anything in the paper, so the published work-type split should be read as a rough triage signal rather than a measured distribution. The impact is bounded but not removed: since 2026-10-23 the engine excludes floor-confidence records from the shortlist entirely and publishes them separately as held out, so the recommendation is unaffected even though the census is.","status":"unverified","severity":"high"},{"code":"runtime_is_modelled","basis":["Runtime is summed from published per-assay hour figures. Timing awaits the first executed replication.","4,407 of 5,529 records (79.7%) model to 0.00 hours because no assay was matched, which the ranking then reads as \"cheapest\".","0 of 10 shortlisted records carry a 0.00-hour estimate."],"claim":"Estimated replication runtime","gloss":"The hour figures are estimates built from assay mentions; timing awaits the first executed replication.","detail":"The hour figures are a cost model derived from the bench steps implied by each matched assay family. The first executed replication will supply measured timing. Records without an assay match currently receive a zero-hour model value.","status":"unverified","severity":"high"},{"code":"signal_score_without_citations","basis":["5,529 of 5,529 records (100.0%) have no citation count from the source metadata."],"claim":"Signal score","gloss":"The signal score uses publication-type weight because the mirror supplied zero citation counts.","detail":"The source supplied no citation counts for any record in this run, so the signal score is publication-type weight only. The citation term of the ranking is inert here, and the shortlist is ordered by publication type alone.","status":"unverified","severity":"high"},{"code":"abstract_absent","basis":["1,667 of 5,529 records (30.2%) carry no abstract at all."],"claim":"Evidence base for classification","gloss":"Some records contain a title and publication type only, defining the available classification evidence.","detail":"Where the mirror contains a title and publication types only, those fields define the classifier evidence. A methods section is required for fuller automated and human review.","status":"unverified","severity":"medium"},{"code":"classifier_agreement_measured","basis":["Published two-way decision agreement 77.7% (310 of 399 decidable records) from a stratified hand-check of 438 real records.","Three-way diagnostic agreement 73.9% (288 of 390 decidable records).","data_only agreement 75.2% (100 of 133 decidable).","48 record(s) could not be decided from title and abstract and were excluded from the denominator.","56 analysis-only records were published under another work type, so the data_only share is not an upper bound."],"claim":"Published automatable decision","gloss":"This figure has been checked against a human reading the same papers, so we know how often it is right.","detail":"The published automatable / not-automatable distribution comes from a rule-based classifier that was hand-checked against a fixed ground-truth definition. The agreement rates below are measured, not estimated. The three-way work type remains a diagnostic and is reported separately.","status":"measured","severity":"high"},{"code":"llm_comparison_not_measured","basis":["rule_based_v1+lex99e1195c7d6f produced 5,529 of 5,529 classifications.","Measured on 438 labelled records; these are RULE-BASED figures, not model results."],"claim":"Rule-based versus LLM agreement","gloss":"Every number here is the rule engine’s own. Model comparison begins when a working API credential is connected and measured.","detail":"The published two-way decision agreed with a human reading on 310 of 399 decidable records (77.7%). The diagnostic three-way label agreed on 288 of 390 decidable records (73.9%). Every percentage in this run belongs to the rule engine. Model comparison begins when a working API credential is connected and measured.","status":"not_measured","severity":"high"},{"code":"confidence_is_a_band","basis":["low band: 135 of 210 hand-judged records were correct, 64.3% [57.6, 70.5] (95% CI).","medium band: 80 of 104 hand-judged records were correct, 76.9% [68.0, 84.0] (95% CI).","high band: 73 of 76 hand-judged records were correct, 96.0% [89.0, 98.7] (95% CI).","A fitted calibrated probability was built, measured and withdrawn: it overstated held-out accuracy and reorders nothing."],"claim":"Confidence band and evidence score","gloss":"The confidence band carries a measured accuracy interval; 0.85 represents relative rank strength.","detail":"Confidence on this run is a BAND plus its measured accuracy interval. The number stored alongside each record is a monotone evidence score that orders records; 0.85 represents relative rank strength. A fitted probability was withdrawn after it overstated held-out accuracy. Threshold on the band.","status":"measured","severity":"medium"},{"code":"three_way_split_is_diagnostic","basis":["The corpus is published at the two-way decision: automatable or not-automatable. The three-way split (data_only / physical / mixed) is published as a diagnostic with its measured reliability attached, not as a claim, because the rule engine's `mixed` call cannot be defended.","The engine called 22 record(s) mixed; 10 matched the human judgement (45.5%, 95% CI 26.9-65.3).","The engine's `mixed` call is right on 10/22 hand-judged records (45.5%, 95% CI 26.9%-65.3%); a constant `physical` guess scores 36.4%.","The engine's `mixed` call does not reliably beat a constant `physical` guess on the hand-judged records it labels mixed.","The sign of that difference flips between two independent samples, so the three-way refinement is not demonstrably informative.","The mixed stratum contributes a disproportionate share of errors relative to its size.","At title-only depth the physical/mixed split collapses: a title says that work was done, not whether it was also analysed."],"claim":"The published decision is two-way","gloss":"The three-way split is shown for diagnosis only. The published split is two ways.","detail":"Count and filter the corpus on automatable versus not-automatable. The data_only / physical / mixed split remains an internal engine diagnostic because the mixed call has lower measured reliability.","status":"measured","severity":"medium"},{"code":"automatable_flag_unproven","basis":["6 of 29 assay families are marked automatable now: meta_analysis, machine_learning, survival_analysis, pca, molecular_docking, differential_expression.","The flag is a rule over assay family and work type; validation begins with the first executed replication.","It inherits the 75.2% data_only agreement rate, because the flag requires the record to be analysis-only."],"claim":"Automatable now","gloss":"This flag marks assay families the rules classify as data-only. The first executed replication will validate it.","detail":"This flag marks families the rules consider replicable from data alone. Its validity depends on the analysis-only classification and will be measured through an executed replication.","status":"unverified","severity":"medium"}],"confidence_policy":"band_with_measured_interval","confidence_contract":{"policy":"band_with_measured_interval","summary":"Confidence is reported as a BAND with a measured accuracy interval. The raw evidence score supplies monotone ordering. A fitted point estimate was withdrawn after it overstated held-out accuracy.","evidence_score":"A monotone evidence score in [0,1] built from cue mass, axis separation and text coverage. Use it to order records. Interpret 0.85 as relative rank strength.","confidence_band":"low | medium | high. Consumers threshold on this value.","band_accuracy":"Wilson 95% interval on the measured agreement of the hand-judged records in this band.","calibrated_confidence":"WITHDRAWN. Reintroduction requires held-out evidence demonstrating reliable estimates across every bin."},"band_accuracy":{"low":{"n":210,"low":0.576,"band":"low","high":0.7046,"agree":135,"accuracy":0.6429},"high":{"n":76,"low":0.8903,"band":"high","high":0.9865,"agree":73,"accuracy":0.9605},"medium":{"n":104,"low":0.6795,"band":"medium","high":0.8397,"agree":80,"accuracy":0.7692}},"provider":"rule_based_v1+lex99e1195c7d6f","lexicon_id":"99e1195c7d6f","contract_published_at":"2026-10-23T12:25:29.022232+00:00","confidence_is_band":true,"confidence_is_probability":false,"decision_taxonomy":"two_way","decidable_fraction_two_way":0.8854,"decidable_fraction_two_way_low":0.8565,"decidable_fraction_two_way_high":0.9143,"decidable_fraction_three_way":0.8586,"decidable_fraction_three_way_low":0.8287,"decidable_fraction_three_way_high":0.8884,"decidable_population":5529,"validation_sample_size":438,"three_way_cost_fraction":0.0268,"three_way_cost_records":148,"decision_counts":{"automatable":230,"not_automatable":5299},"decision_split":{"automatable":{"count":230,"share":0.04159884246699222},"not_automatable":{"count":5299,"share":0.9584011575330078}},"diagnostic_work_type_counts":{"mixed":24,"physical":5275,"data_only":230}}}