{"data":{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37663293", "kind": "science-record", "revision": 4, "content_hash": "sha256:cc5b52272a45c4e01bcc721a4407958d211645833c81d394c2a1da6bbd53be4d", "created_at": "2026-11-01T19:02:27Z", "updated_at": "2026-11-01T22:52:38Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of Zip8-correlated hub genes in pulmonary hypertension by informatic analysis.", "doi": "10.7717/peerj.15939", "pmid": "37663293", "authors": ["FanRong Zhao", "Yujing Chen", "Yuliang Xie", "Shuang Kong", "LiaoFan Song", "Hanfei Li", "Chao Guo", "Yanyan Yin", "Weifang Zhang", "Tiantian Zhu"], "journal": "PeerJ", "year": 2023, "abstract": "Background: Pulmonary hypertension (PH) is a syndrome characterized by marked remodeling of the pulmonary vasculature and increased pulmonary vascular resistance, ultimately leading to right heart failure and even death. The localization of Zrt/Irt-like Protein 8 (ZIP8, a metal ion transporter, encoded by SLC39A8) was abundantly in microvasculature endothelium and its pivotal role in the lung has been demonstrated. However, the role of Zip8 in PH remains unclear.\nMethods: Bioinformatics analysis was employed to identify SLC39A8 expression patterns and differentially expressed genes (DEGs) between PH patients and normal controls (NC), based on four datasets (GSE24988, GSE113439, GSE117261, and GSE15197) from the Biotechnology Gene Expression Omnibus (NCBI GEO) database. Gene set enrichment analysis (GSEA) was performed to analyze signaling pathways enriched for DEGs. Hub genes were identified by cytoHubba analysis in Cytoscape. Reverse transcriptase-polymerase chain reaction was used to validate SLC39A8 and its correlated metabolic DEGs expression in PH (SU5416/Hypoxia) mice.\nResults: SLC39A8 expression was downregulated in PH patients, and this expression pattern was validated in PH (SU5416/Hypoxia) mouse lung tissue. SLC39A8-correlated genes were mainly enriched in the metabolic pathways. Within these SLC39A8-correlated genes, 202 SLC39A8-correlated metabolic genes were screened out, and seven genes were identified as SLC39A8-correlated metabolic hub genes. The expression patterns of hub genes were analyzed between PH patients and controls and further validated in PH mice. Finally, four genes (Fasn, Nsdhl, Acat2, and Acly) were downregulated in PH mice. However, there were no significant differences in the expression of the other three hub genes between PH mice and controls. Of the four genes, Fasn and Acly are key enzymes in fatty acids synthesis, Nsdhl is involved in cholesterol synthesis, and Acat2 is implicated in cholesterol metabolic transformation. Taken together, these results provide novel insight into the role of Zip8 in PH.", "claims": [{"id": "claim-method", "statement": "Wilcoxon rank sum test and Welch t’ test was used to compare the difference of SLC39A8 and 7 key hub genes expression between PH and NC. Pearson correlation analysis was used to examine the relationship between the expression of SLC39A8 and other genes expressed in lung tissue of PH and NC. A Student’s t-test was used to compare the difference in SLC39A8, Acat2, Acly, and Fasn expression in the lung between PH mice and control mice.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37663293", "locator": "sections[9] \"Statistical analysis\" paragraph 2", "quote": "Wilcoxon rank sum test and Welch t’ test was used to compare the difference of SLC39A8 and 7 key hub genes expression between PH and NC. Pearson correlation analysis was used to examine the relationship between the expression of SLC39A8 and other genes expressed in lung tissue of PH and NC. A Student’s t-test was used to compare the difference in SLC39A8, Acat2, Acly, and Fasn expression in the lung between PH mice and control mice."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37663293", "locator": "sections[20] \"Additional Information and Declarations\" paragraph 5", "quote": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37663293"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE113439"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE113439"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37663293", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.7717/peerj.15939", "sha256": "sha256:f8b715aec3a7eb8cb0ebdcf8e77d3433bfb2698b612ef44aa06569cadf821659", "bytes": 33154, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.7717/peerj.15939", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10470448", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE113439", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE113439", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE113439", "version": null, "note": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM3106326", "GSM3106327", "GSM3106328", "GSM3106329", "GSM3106330", "GSM3106331", "GSM3106332", "GSM3106333", "GSM3106334", "GSM3106335", "GSM3106336", "GSM3106337", "GSM3106338", "GSM3106339", "GSM3106340", "GSM3106341", "GSM3106342", "GSM3106343", "GSM3106344", "GSM3106345", "GSM3106346", "GSM3106347", "GSM3106348", "GSM3106349", "GSM3106350", "GSM3106351"], "sample_count": 26, "group_sizes": null, "group_sizes_basis": null, "platform": "6244", "taxon": "Homo sapiens", "title": "Gene expression profiling of pulmonary arterial hypertension", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE117261", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE117261", "version": null, "note": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM3290067", "GSM3290068", "GSM3290069", "GSM3290070", "GSM3290071", "GSM3290072", "GSM3290073", "GSM3290074", "GSM3290075", "GSM3290076", "GSM3290077", "GSM3290078", "GSM3290079", "GSM3290080", "GSM3290081", "GSM3290082", "GSM3290083", "GSM3290084", "GSM3290085", "GSM3290086", "GSM3290087", "GSM3290088", "GSM3290089", "GSM3290090", "GSM3290091", "GSM3290092", "GSM3290093", "GSM3290094", "GSM3290095", "GSM3290096", "GSM3290097", "GSM3290098", "GSM3290099", "GSM3290100", "GSM3290101", "GSM3290102", "GSM3290103", "GSM3290104", "GSM3290105", "GSM3290106", "GSM3290107", "GSM3290108", "GSM3290109", "GSM3290110", "GSM3290111", "GSM3290112", "GSM3290113", "GSM3290114", "GSM3290115", "GSM3290116", "GSM3290117", "GSM3290118", "GSM3290119", "GSM3290120", "GSM3290121", "GSM3290122", "GSM3290123", "GSM3290124", "GSM3290125", "GSM3290126", "GSM3290127", "GSM3290128", "GSM3290129", "GSM3290130", "GSM3290131", "GSM3290132", "GSM3290133", "GSM3290134", "GSM3290135", "GSM3290136", "GSM3290137", "GSM3290138", "GSM3290139", "GSM3290140", "GSM3290141", "GSM3290142", "GSM3290143", "GSM3290144", "GSM3290145", "GSM3290146", "GSM3290147", "GSM3290148", "GSM3290149"], "sample_count": 83, "group_sizes": null, "group_sizes_basis": null, "platform": "6244", "taxon": "Homo sapiens", "title": "Systems Analysis of the Human Pulmonary Arterial Hypertension Lung Transcriptome", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE15197", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15197", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE15197", "version": null, "note": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM379316", "GSM379317", "GSM379318", "GSM379319", "GSM379320", "GSM379321", "GSM379322", "GSM379323", "GSM379324", "GSM379325", "GSM379326", "GSM379327", "GSM379328", "GSM379329", "GSM379330", "GSM379331", "GSM379332", "GSM379333", "GSM379334", "GSM379335", "GSM379336", "GSM379337", "GSM379338", "GSM379339", "GSM379340", "GSM379341", "GSM379342", "GSM379343", "GSM379344", "GSM379345", "GSM379346", "GSM379347", "GSM379348", "GSM379349", "GSM379350", "GSM379351", "GSM379352", "GSM379353", "GSM379354"], "sample_count": 39, "group_sizes": null, "group_sizes_basis": null, "platform": "6480", "taxon": "Homo sapiens", "title": "RNA Expression Profiling of Lung Tissue Identifies Mutually Distinct Molecular Signatures in PAH and PH Secondary to IPF", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE24988", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24988", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE24988", "version": null, "note": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM613638", "GSM613639", "GSM613640", "GSM613641", "GSM613642", "GSM613643", "GSM613644", "GSM613645", "GSM613646", "GSM613647", "GSM613648", "GSM613649", "GSM613650", "GSM613651", "GSM613652", "GSM613653", "GSM613654", "GSM613655", "GSM613656", "GSM613657", "GSM613658", "GSM613659", "GSM613660", "GSM613661", "GSM613662", "GSM613663", "GSM613664", "GSM613665", "GSM613666", "GSM613667", "GSM613668", "GSM613669", "GSM613670", "GSM613671", "GSM613672", "GSM613673", "GSM613674", "GSM613675", "GSM613676", "GSM613677", "GSM613678", "GSM613679", "GSM613680", "GSM613681", "GSM613682", "GSM613683", "GSM613684", "GSM613685", "GSM613686", "GSM613687", "GSM613688", "GSM613689", "GSM613690", "GSM613691", "GSM613692", "GSM613693", "GSM613694", "GSM613695", "GSM613696", "GSM613697", "GSM613698", "GSM613699", "GSM613700", "GSM613701", "GSM613702", "GSM613703", "GSM613704", "GSM613705", "GSM613706", "GSM613707", "GSM613708", "GSM613709", "GSM613710", "GSM613711", "GSM613712", "GSM613713", "GSM613714", "GSM613715", "GSM613716", "GSM613717", "GSM613718", "GSM613719", "GSM613720", "GSM613721", "GSM613722", "GSM613723", "GSM613724", "GSM613725", "GSM613726", "GSM613727", "GSM613728", "GSM613729", "GSM613730", "GSM613731", "GSM613732", "GSM613733", "GSM613734", "GSM613735", "GSM613736", "GSM613737", "GSM613738", "GSM613739", "GSM613740", "GSM613741", "GSM613742", "GSM613743", "GSM613744", "GSM613745", "GSM613746", "GSM613747", "GSM613748", "GSM613749", "GSM613750", "GSM613751", "GSM613752", "GSM613753"], "sample_count": 116, "group_sizes": null, "group_sizes_basis": null, "platform": "6244", "taxon": "Homo sapiens", "title": "Gene expression profiles based on Pulmonary Artery Pressures in Pulmonary Fibrosis", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE113439 not downloaded", "asset:data:geo:GSE113439 licence not read", "asset:data:geo:GSE117261 not downloaded", "asset:data:geo:GSE117261 licence not read", "asset:data:geo:GSE15197 not downloaded", "asset:data:geo:GSE15197 licence not read", "asset:data:geo:GSE24988 not downloaded", "asset:data:geo:GSE24988 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "welch_t_test", "label": "Group comparison", "dsl_operations": ["welch_t"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Wilcoxon rank sum test and Welch t’ test was used to compare the difference of SLC39A8 and 7 key hub genes expression between PH and NC. Pearson correlation analysis was used to examine the relationship between the expression of SLC39A8 and other genes expressed in lung tissue of PH and NC. A Student’s t-test was used to compare the difference in SLC39A8, Acat2, Acly, and Fasn expression in the lung between PH mice and control mice."}}, {"id": "method-2", "family": "group_comparison", "specific": "wilcoxon", "label": "Group comparison", "dsl_operations": [], "missing_operations": ["group_comparison_wilcoxon"], "expressible": false, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "Wilcoxon"}}, {"id": "method-3", "family": "correlation_analysis", "specific": "pearson", "label": "Correlation analysis", "dsl_operations": ["pearson_r"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["paired_numeric_columns"], "evidence": {"score": 0.8, "band": "high", "quote": "Pearson correlation"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["welch_t"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: welch_t_test."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE24988", "GSE113439", "GSE117261", "GSE15197"], "resolved_accessions": ["GSE24988", "GSE113439", "GSE117261", "GSE15197"], "statements": ["The raw data is available in the Supplemental Files.The microarray data are available at GEO: GSE24988, GSE113439, GSE117261, GSE15197."], "basis": "The paper names GSE24988, GSE113439, GSE117261, GSE15197 in Biotechnology Gene Expression Omnibus (NCBI GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["welch_t"], "assets": ["source:pmid:37663293", "data:geo:GSE113439"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE113439"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T18:52:16Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 10085, "completion_tokens": 2736, "cached_tokens": 264}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:37663293", "type": "paper", "label": "pmid:37663293"}, {"id": "source:pmid:37663293", "type": "manuscript", "label": "PMID 37663293 full text"}, {"id": "data:geo:GSE113439", "type": "dataset", "label": "GEO GSE113439"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:37663293", "type": "paper", "label": "pmid:37663293"}, {"id": "source:pmid:37663293", "type": "manuscript", "label": "PMID 37663293 full text"}, {"id": "data:geo:GSE113439", "type": "dataset", "label": "GEO GSE113439"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-37663293", "type": "extraction", "used": ["source:pmid:37663293"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE113439", "type": "accession_resolution", "used": ["data:geo:GSE113439"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:37663293", "parent_hash": "sha256:0db0bb662d660f0d98b096480e0a9fed849f422431f70a1923d7e19d0474f731", "fork_of": null, "generation": 3}, "gaps": ["asset:data:geo:GSE113439 licence not read", "asset:data:geo:GSE113439 not downloaded", "asset:data:geo:GSE117261 licence not read", "asset:data:geo:GSE117261 not downloaded", "asset:data:geo:GSE15197 licence not read", "asset:data:geo:GSE15197 not downloaded", "asset:data:geo:GSE24988 licence not read", "asset:data:geo:GSE24988 not downloaded", "execution.not_run", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["pattern was validated in PH (SU5416/Hypoxia) mouse lung tissue. SLC39A8-correlated genes were m", "Materials & Methods Mouse model of PH/animal experiment C57BL/6J male mice a", "ed in carboxymethylcellulose solution). Then mice were housed in a hypoxic environment (10% O2, Hx)", "euthanasia. At the end of the treatment, all mice were anaesthetized with pentobarbital sodium (30"], "order_basis": "paper_text_order:1268", "id": "assay:1"}, {"role": "data_generation", "definition_id": "clinical_trial", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["PF (Beijing) Biotechnology Co., Ltd. Animals were randomized into two groups, kept at 20–25 °C under a 12 h li"], "order_basis": "paper_text_order:2276", "id": "assay:2"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["welch_t"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Wilcoxon rank sum test and Welch t’ test was used to compare the difference of SLC39A8 and 7 key hub genes expression between PH and NC. Pearson correlation analysis was used to examine the relationship between the expression of SLC39A8 and other genes expressed in lung tissue of PH and NC. A Student’s t-test was used to compare the difference in SLC39A8, Acat2, Acly, and Fasn expression in the lung between PH mice and control mice."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]},"meta":{"api_version":"v1","automation_ladder_snapshot":"ba34b49490d9bfaf","automation_ladder_caveats":["Method extraction is rule-based and has no hand-judged accuracy sample. Evidence bands order textual support; method agreement intervals are null (n=0), never probabilities. Work-type validation does not validate methods.","Operation coverage describes only explicitly named methods. It does not prove a complete protocol, compatible parameters, successful compilation or re-execution. Unrecognised or ambiguous methods require review.","Data reachability is a textual accession or public-data statement. No dataset was downloaded or checked for access, completeness or licence.","Capabilities are planning states seeded from the dated equipment register. No bench ownership, completed quote or partner booking is inferred. Procurement counts are dependencies, not guaranteed replications or financial returns.","Tier and blocker counts are deterministic assessments of this corpus, not measured agreement. R3 is reserved for procurement; unresolved methods and data require human work (R4). The most restrictive axis wins, with capability, method, data as tie-breaks. Resolving one blocker can leave another at the same tier."],"engine":"corpus_engine","engine_provider":"rule_based_v1+lex99e1195c7d6f","run_id":"run-20261023T122449232913","source":"esearch","source_detail":"PubMed mirror biomed_pubmed (read-only)","run_status":"complete","imported_at":"2026-11-03T20:16:52+00:00","generated_at":"2026-11-04T00:29:56+00:00","total_papers":5529,"total_assays_tracked":29,"automatable_assay_families":6,"validated":true,"caveat_count":9,"blocking_caveats":5,"caveats":[{"code":"low_evidence_dominates","basis":["4,152 of 5,529 classifications (75.1%) matched no cue evidence and sit at the 0.05 floor.","34 of 230 data_only calls (14.8%) are in that group."],"claim":"Work type and confidence bands","gloss":"Most records were classified from their title and framing alone, with assay-cue coverage at zero.","detail":"Most classifications in this run rest on no matched evidence at all. At the evidence floor the work type comes from title framing and a fixed prior, not from anything in the paper, so the published work-type split should be read as a rough triage signal rather than a measured distribution. The impact is bounded but not removed: since 2026-10-23 the engine excludes floor-confidence records from the shortlist entirely and publishes them separately as held out, so the recommendation is unaffected even though the census is.","status":"unverified","severity":"high"},{"code":"runtime_is_modelled","basis":["Runtime is summed from published per-assay hour figures. Timing awaits the first executed replication.","4,407 of 5,529 records (79.7%) model to 0.00 hours because no assay was matched, which the ranking then reads as \"cheapest\".","0 of 10 shortlisted records carry a 0.00-hour estimate."],"claim":"Estimated replication runtime","gloss":"The hour figures are estimates built from assay mentions; timing awaits the first executed replication.","detail":"The hour figures are a cost model derived from the bench steps implied by each matched assay family. The first executed replication will supply measured timing. Records without an assay match currently receive a zero-hour model value.","status":"unverified","severity":"high"},{"code":"signal_score_without_citations","basis":["5,529 of 5,529 records (100.0%) have no citation count from the source metadata."],"claim":"Signal score","gloss":"The signal score uses publication-type weight because the mirror supplied zero citation counts.","detail":"The source supplied no citation counts for any record in this run, so the signal score is publication-type weight only. The citation term of the ranking is inert here, and the shortlist is ordered by publication type alone.","status":"unverified","severity":"high"},{"code":"abstract_absent","basis":["1,667 of 5,529 records (30.2%) carry no abstract at all."],"claim":"Evidence base for classification","gloss":"Some records contain a title and publication type only, defining the available classification evidence.","detail":"Where the mirror contains a title and publication types only, those fields define the classifier evidence. A methods section is required for fuller automated and human review.","status":"unverified","severity":"medium"},{"code":"classifier_agreement_measured","basis":["Published two-way decision agreement 77.7% (310 of 399 decidable records) from a stratified hand-check of 438 real records.","Three-way diagnostic agreement 73.9% (288 of 390 decidable records).","data_only agreement 75.2% (100 of 133 decidable).","48 record(s) could not be decided from title and abstract and were excluded from the denominator.","56 analysis-only records were published under another work type, so the data_only share is not an upper bound."],"claim":"Published automatable decision","gloss":"This figure has been checked against a human reading the same papers, so we know how often it is right.","detail":"The published automatable / not-automatable distribution comes from a rule-based classifier that was hand-checked against a fixed ground-truth definition. The agreement rates below are measured, not estimated. The three-way work type remains a diagnostic and is reported separately.","status":"measured","severity":"high"},{"code":"llm_comparison_not_measured","basis":["rule_based_v1+lex99e1195c7d6f produced 5,529 of 5,529 classifications.","Measured on 438 labelled records; these are RULE-BASED figures, not model results."],"claim":"Rule-based versus LLM agreement","gloss":"Every number here is the rule engine’s own. Model comparison begins when a working API credential is connected and measured.","detail":"The published two-way decision agreed with a human reading on 310 of 399 decidable records (77.7%). The diagnostic three-way label agreed on 288 of 390 decidable records (73.9%). Every percentage in this run belongs to the rule engine. Model comparison begins when a working API credential is connected and measured.","status":"not_measured","severity":"high"},{"code":"confidence_is_a_band","basis":["low band: 135 of 210 hand-judged records were correct, 64.3% [57.6, 70.5] (95% CI).","medium band: 80 of 104 hand-judged records were correct, 76.9% [68.0, 84.0] (95% CI).","high band: 73 of 76 hand-judged records were correct, 96.0% [89.0, 98.7] (95% CI).","A fitted calibrated probability was built, measured and withdrawn: it overstated held-out accuracy and reorders nothing."],"claim":"Confidence band and evidence score","gloss":"The confidence band carries a measured accuracy interval; 0.85 represents relative rank strength.","detail":"Confidence on this run is a BAND plus its measured accuracy interval. The number stored alongside each record is a monotone evidence score that orders records; 0.85 represents relative rank strength. A fitted probability was withdrawn after it overstated held-out accuracy. Threshold on the band.","status":"measured","severity":"medium"},{"code":"three_way_split_is_diagnostic","basis":["The corpus is published at the two-way decision: automatable or not-automatable. The three-way split (data_only / physical / mixed) is published as a diagnostic with its measured reliability attached, not as a claim, because the rule engine's `mixed` call cannot be defended.","The engine called 22 record(s) mixed; 10 matched the human judgement (45.5%, 95% CI 26.9-65.3).","The engine's `mixed` call is right on 10/22 hand-judged records (45.5%, 95% CI 26.9%-65.3%); a constant `physical` guess scores 36.4%.","The engine's `mixed` call does not reliably beat a constant `physical` guess on the hand-judged records it labels mixed.","The sign of that difference flips between two independent samples, so the three-way refinement is not demonstrably informative.","The mixed stratum contributes a disproportionate share of errors relative to its size.","At title-only depth the physical/mixed split collapses: a title says that work was done, not whether it was also analysed."],"claim":"The published decision is two-way","gloss":"The three-way split is shown for diagnosis only. The published split is two ways.","detail":"Count and filter the corpus on automatable versus not-automatable. The data_only / physical / mixed split remains an internal engine diagnostic because the mixed call has lower measured reliability.","status":"measured","severity":"medium"},{"code":"automatable_flag_unproven","basis":["6 of 29 assay families are marked automatable now: meta_analysis, machine_learning, survival_analysis, pca, molecular_docking, differential_expression.","The flag is a rule over assay family and work type; validation begins with the first executed replication.","It inherits the 75.2% data_only agreement rate, because the flag requires the record to be analysis-only."],"claim":"Automatable now","gloss":"This flag marks assay families the rules classify as data-only. The first executed replication will validate it.","detail":"This flag marks families the rules consider replicable from data alone. Its validity depends on the analysis-only classification and will be measured through an executed replication.","status":"unverified","severity":"medium"}],"confidence_policy":"band_with_measured_interval","confidence_contract":{"policy":"band_with_measured_interval","summary":"Confidence is reported as a BAND with a measured accuracy interval. The raw evidence score supplies monotone ordering. A fitted point estimate was withdrawn after it overstated held-out accuracy.","evidence_score":"A monotone evidence score in [0,1] built from cue mass, axis separation and text coverage. Use it to order records. Interpret 0.85 as relative rank strength.","confidence_band":"low | medium | high. Consumers threshold on this value.","band_accuracy":"Wilson 95% interval on the measured agreement of the hand-judged records in this band.","calibrated_confidence":"WITHDRAWN. Reintroduction requires held-out evidence demonstrating reliable estimates across every bin."},"band_accuracy":{"low":{"n":210,"low":0.576,"band":"low","high":0.7046,"agree":135,"accuracy":0.6429},"high":{"n":76,"low":0.8903,"band":"high","high":0.9865,"agree":73,"accuracy":0.9605},"medium":{"n":104,"low":0.6795,"band":"medium","high":0.8397,"agree":80,"accuracy":0.7692}},"provider":"rule_based_v1+lex99e1195c7d6f","lexicon_id":"99e1195c7d6f","contract_published_at":"2026-10-23T12:25:29.022232+00:00","confidence_is_band":true,"confidence_is_probability":false,"decision_taxonomy":"two_way","decidable_fraction_two_way":0.8854,"decidable_fraction_two_way_low":0.8565,"decidable_fraction_two_way_high":0.9143,"decidable_fraction_three_way":0.8586,"decidable_fraction_three_way_low":0.8287,"decidable_fraction_three_way_high":0.8884,"decidable_population":5529,"validation_sample_size":438,"three_way_cost_fraction":0.0268,"three_way_cost_records":148,"decision_counts":{"automatable":230,"not_automatable":5299},"decision_split":{"automatable":{"count":230,"share":0.04159884246699222},"not_automatable":{"count":5299,"share":0.9584011575330078}},"diagnostic_work_type_counts":{"mixed":24,"physical":5275,"data_only":230}}}