{"data":{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40646297", "kind": "science-record", "revision": 4, "content_hash": "sha256:fa87019103885574472e6d2ead61dd7de4a2e18e850d92e864e6ce637ed152e6", "created_at": "2026-11-01T20:30:44Z", "updated_at": "2026-11-01T22:56:49Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrated bioinformatics identifies ferroptosis biomarkers and therapeutic targets in idiopathic pulmonary arterial hypertension.", "doi": "10.1038/s41598-025-11066-y", "pmid": "40646297", "authors": ["Yuhao Zhang", "Tao Qian", "Wei Jiang", "Haoyong Yuan", "Ting Lu", "Ni Yin", "Zhongshi Wu", "Can Huang"], "journal": "Scientific reports", "year": 2025, "abstract": "Idiopathic pulmonary arterial hypertension (IPAH), a rare and devastating pulmonary vascular disorder, is characterized by cellular proliferation and vascular remodeling. Although previous studies have underscored that ferroptosis, an iron-dependent cell death process, plays an important regulatory role in pulmonary artery hypertension, its role remains understudied. Gene expression profiles were downloaded from the Gene Expression Omnibus(GEO). Differentially expressed genes (DEGs) were screened using R software and intersected with a ferroptosis database (FerrDb V1) to identify ferroptosis-related DEGs. GO and KEGG analyses were performed to explore biological functions and potential pathways. LASSO and SVM-RFE algorithms were used to identify optimal gene biomarkers for IPAH. GSVA and GSEA were conducted to explore biological functions and potential pathways associated with these biomarkers. The CIBESORT software was employed to predict immune genes and functions. Of 237 ferroptosis-related genes (FRGs), 27 differentially expressed FRGs (DE-FRGs) showed significant differences between IPAH and normal samples in GSE48149, with 15 downregulated and 12 upregulated genes. Six DE-FRGs, including KEAP1, TNFAIP3, MEG3, NFS1, PRDX1, and BEX1, were identified as predictive diagnostic genes for IPAH. Among these DE-FRGs, PRDX1 and TNFAIP3 were the most promising diagnostic genes for IPAH and may play a corresponding role in IPAH by participating in the cell cycle, lysosomes, immune response, vascular smooth muscle contraction, and various diseases. CIBERSORT analysis revealed a positive correlation between neutrophils and TNFAIP3, whereas macrophages M0 exhibited a negative correlation with PRDX1. Through comprehensive bioinformatics analysis, we identified six differentially expressed ferroptosis-related genes (DE-FRGs)-KEAP1, TNFAIP3, MEG3, NFS1, PRDX1, and BEX1-in idiopathic pulmonary arterial hypertension (IPAH). Among these, PRDX1 and TNFAIP3, which play key roles in IPAH pathogenesis, emerged as the most promising diagnostic biomarkers.", "claims": [{"id": "claim-data-access", "statement": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40646297", "locator": "sections[31] \"notes\" paragraph 1", "quote": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Analysis of GSE48149 identified 27 differentially expressed ferroptosis-related genes between IPAH and normal samples, comprising 15 downregulated and 12 upregulated genes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40646297", "locator": "sections[16] \"Identification of DE-FRGs from GSE48149\" paragraph 2", "quote": "Of 237 FRGs, 27 DE-FRGs exhibited a significant difference between IPAH and normal samples in GSE48149, with 15 downregulated and 12 upregulated genes (Table 1), visualized using a heatmap (Fig. 1A)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Analysis of GSE48149 identified 27 differentially expressed ferroptosis-related genes between IPAH and normal samples, comprising 15 downregulated and 12 upregulated genes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40646297"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE117261"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE117261"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:40646297", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1038/s41598-025-11066-y", "sha256": "sha256:766a5bbffbfbf96c5b558c5d6bd9e359857c8b258d803189ebb24784690cbb7d", "bytes": 45498, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1038/s41598-025-11066-y", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC12254406", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE117261", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE117261", "version": null, "note": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM3290067", "GSM3290068", "GSM3290069", "GSM3290070", "GSM3290071", "GSM3290072", "GSM3290073", "GSM3290074", "GSM3290075", "GSM3290076", "GSM3290077", "GSM3290078", "GSM3290079", "GSM3290080", "GSM3290081", "GSM3290082", "GSM3290083", "GSM3290084", "GSM3290085", "GSM3290086", "GSM3290087", "GSM3290088", "GSM3290089", "GSM3290090", "GSM3290091", "GSM3290092", "GSM3290093", "GSM3290094", "GSM3290095", "GSM3290096", "GSM3290097", "GSM3290098", "GSM3290099", "GSM3290100", "GSM3290101", "GSM3290102", "GSM3290103", "GSM3290104", "GSM3290105", "GSM3290106", "GSM3290107", "GSM3290108", "GSM3290109", "GSM3290110", "GSM3290111", "GSM3290112", "GSM3290113", "GSM3290114", "GSM3290115", "GSM3290116", "GSM3290117", "GSM3290118", "GSM3290119", "GSM3290120", "GSM3290121", "GSM3290122", "GSM3290123", "GSM3290124", "GSM3290125", "GSM3290126", "GSM3290127", "GSM3290128", "GSM3290129", "GSM3290130", "GSM3290131", "GSM3290132", "GSM3290133", "GSM3290134", "GSM3290135", "GSM3290136", "GSM3290137", "GSM3290138", "GSM3290139", "GSM3290140", "GSM3290141", "GSM3290142", "GSM3290143", "GSM3290144", "GSM3290145", "GSM3290146", "GSM3290147", "GSM3290148", "GSM3290149"], "sample_count": 83, "group_sizes": null, "group_sizes_basis": null, "platform": "6244", "taxon": "Homo sapiens", "title": "Systems Analysis of the Human Pulmonary Arterial Hypertension Lung Transcriptome", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE169471", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE169471", "version": null, "note": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM5206779", "GSM5206780", "GSM5206781", "GSM5206782", "GSM5206783", "GSM5206784", "GSM5206785", "GSM5206786", "GSM5206787", "GSM5206788", "GSM5206789"], "sample_count": 11, "group_sizes": null, "group_sizes_basis": null, "platform": "20795;18573", "taxon": "Homo sapiens", "title": "Transcriptional profiling of lung cell populations in idiopathic pulmonary arterial hypertension", "resolution_checked_at": "2026-11-01T19:09:46Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE33463", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE33463", "version": null, "note": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM827665", "GSM827666", "GSM827667", "GSM827668", "GSM827669", "GSM827670", "GSM827671", "GSM827672", "GSM827673", "GSM827674", "GSM827675", "GSM827676", "GSM827677", "GSM827678", "GSM827679", "GSM827680", "GSM827681", "GSM827682", "GSM827683", "GSM827684", "GSM827685", "GSM827686", "GSM827687", "GSM827688", "GSM827689", "GSM827690", "GSM827691", "GSM827692", "GSM827693", "GSM827694", "GSM827695", "GSM827696", "GSM827697", "GSM827698", "GSM827699", "GSM827700", "GSM827701", "GSM827702", "GSM827703", "GSM827704", "GSM827705", "GSM827706", "GSM827707", "GSM827708", "GSM827709", "GSM827710", "GSM827711", "GSM827712", "GSM827713", "GSM827714", "GSM827715", "GSM827716", "GSM827717", "GSM827718", "GSM827719", "GSM827720", "GSM827721", "GSM827722", "GSM827723", "GSM827724", "GSM827725", "GSM827726", "GSM827727", "GSM827728", "GSM827729", "GSM827730", "GSM827731", "GSM827732", "GSM827733", "GSM827734", "GSM827735", "GSM827736", "GSM827737", "GSM827738", "GSM827739", "GSM827740", "GSM827741", "GSM827742", "GSM827743", "GSM827744", "GSM827745", "GSM827746", "GSM827747", "GSM827748", "GSM827749", "GSM827750", "GSM827751", "GSM827752", "GSM827753", "GSM827754", "GSM827755", "GSM827756", "GSM827757", "GSM827758", "GSM827759", "GSM827760", "GSM827761", "GSM827762", "GSM827763", "GSM827764", "GSM827765", "GSM827766", "GSM827767", "GSM827768", "GSM827769", "GSM827770", "GSM827771", "GSM827772", "GSM827773", "GSM827774", "GSM827775", "GSM827776", "GSM827777", "GSM827778", "GSM827779", "GSM827780", "GSM827781", "GSM827782", "GSM827783", "GSM827784", "GSM827785", "GSM827786", "GSM827787", "GSM827788", "GSM827789", "GSM827790", "GSM827791", "GSM827792", "GSM827793", "GSM827794", "GSM827795", "GSM827796", "GSM827797", "GSM827798", "GSM827799", "GSM827800", "GSM827801", "GSM827802", "GSM827803", "GSM827804"], "sample_count": 140, "group_sizes": null, "group_sizes_basis": null, "platform": "6947", "taxon": "Homo sapiens", "title": "Erythroid-Specific Transcriptional Changes in PBMCs from Pulmonary Hypertension Patients", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE48149", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE48149", "version": null, "note": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM1169960", "GSM1169961", "GSM1169962", "GSM1169963", "GSM1169964", "GSM1169965", "GSM1169966", "GSM1169967", "GSM1169968", "GSM1169969", "GSM1169970", "GSM1169971", "GSM1169972", "GSM1169973", "GSM1169974", "GSM1169975", "GSM1169976", "GSM1169977", "GSM1169978", "GSM1169979", "GSM1169980", "GSM1169981", "GSM1169982", "GSM1169983", "GSM1169984", "GSM1169985", "GSM1169986", "GSM1169987", "GSM1169988", "GSM1169989", "GSM1169990", "GSM1169991", "GSM1169992", "GSM1169993", "GSM1169994", "GSM1169995", "GSM1169996", "GSM1169997", "GSM1169998", "GSM1169999", "GSM1170000", "GSM1170001", "GSM1170002", "GSM1170003", "GSM1170004", "GSM1170005", "GSM1170006", "GSM1170007", "GSM1170008", "GSM1170009", "GSM1170010", "GSM1170011", "GSM1170012"], "sample_count": 53, "group_sizes": null, "group_sizes_basis": null, "platform": "16221", "taxon": "Homo sapiens", "title": "Lung tissues in systemic sclerosis have gene expression patterns unique to pulmonary fibrosis and pulmonary hypertension", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE84395", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE84395", "version": null, "note": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM2232981", "GSM2232982", "GSM2232983", "GSM2232984", "GSM2232985", "GSM2232986", "GSM2232987", "GSM2232988", "GSM2232989", "GSM2232990", "GSM2232991", "GSM2232992", "GSM2232993", "GSM2232994", "GSM2232995", "GSM2232996", "GSM2232997", "GSM2232998", "GSM2232999", "GSM2233000", "GSM2233001", "GSM2233002", "GSM2233003", "GSM2233004", "GSM2233005", "GSM2233006", "GSM2233007", "GSM2233008", "GSM2233009", "GSM2233010", "GSM2233011", "GSM2233012", "GSM2233013", "GSM2233014", "GSM2233015", "GSM2233016", "GSM2233017", "GSM2233018", "GSM2233019"], "sample_count": 39, "group_sizes": null, "group_sizes_basis": null, "platform": "16304", "taxon": "Homo sapiens", "title": "Endothelial DNA methalytion signature in pulmonary arterial hypertension", "resolution_checked_at": "2026-11-01T20:16:08Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE117261 not downloaded", "asset:data:geo:GSE117261 licence not read", "asset:data:geo:GSE169471 not downloaded", "asset:data:geo:GSE169471 licence not read", "asset:data:geo:GSE33463 not downloaded", "asset:data:geo:GSE33463 licence not read", "asset:data:geo:GSE48149 not downloaded", "asset:data:geo:GSE48149 licence not read", "asset:data:geo:GSE84395 not downloaded", "asset:data:geo:GSE84395 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "differential_expression", "specific": "limma_voom", "label": "Differential expression analysis", "dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["count_matrix_with_condition"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "The “limma package” in R software (R version 4.2.2) was employed to identify significant DE-FRGs between the two groups using parameters |Log2fold change| >0.5 and adj.P < 0.05 (Table 1)22."}}, {"id": "method-2", "family": "group_comparison", "specific": "wilcoxon", "label": "Group comparison", "dsl_operations": [], "missing_operations": ["group_comparison_wilcoxon"], "expressible": false, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "Wilcoxon"}}, {"id": "method-3", "family": "machine_learning", "specific": "support_vector_machine", "label": "Machine learning", "dsl_operations": ["train_classifier", "cross_validate", "roc_auc"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["labelled_feature_matrix"], "evidence": {"score": 0.7, "band": "medium", "quote": "SVM"}}], "targets": [], "gaps": ["method.reader_differs_from_extractor"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: limma_voom."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE48149", "GSE117261", "GSE84395", "GSE33463", "GSE169471"], "resolved_accessions": ["GSE48149", "GSE117261", "GSE84395", "GSE33463", "GSE169471"], "statements": ["The datasets generated and/or analysed during the current study are available in GSE48149 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48149, GSE117261 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117261, GSE84395 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84395, GSE33463 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33463, GSE169471 repository, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE169471."], "basis": "The paper names GSE48149, GSE117261, GSE84395, GSE33463, GSE169471 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "assets": ["source:pmid:40646297", "data:geo:GSE117261"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE117261"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T19:57:00Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 12385, "completion_tokens": 2653, "cached_tokens": 256}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:40646297", "type": "paper", "label": "pmid:40646297"}, {"id": "source:pmid:40646297", "type": "manuscript", "label": "PMID 40646297 full text"}, {"id": "data:geo:GSE117261", "type": "dataset", "label": "GEO GSE117261"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:40646297", "type": "paper", "label": "pmid:40646297"}, {"id": "source:pmid:40646297", "type": "manuscript", "label": "PMID 40646297 full text"}, {"id": "data:geo:GSE117261", "type": "dataset", "label": "GEO GSE117261"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-40646297", "type": "extraction", "used": ["source:pmid:40646297"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE117261", "type": "accession_resolution", "used": ["data:geo:GSE117261"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:40646297", "parent_hash": "sha256:e1c574b21abc380294b16968ae58f65726bb783cf6ac1b9e7a6e9f6ee3712656", "fork_of": null, "generation": 3}, "gaps": ["asset:data:geo:GSE117261 licence not read", "asset:data:geo:GSE117261 not downloaded", "asset:data:geo:GSE169471 licence not read", "asset:data:geo:GSE169471 not downloaded", "asset:data:geo:GSE33463 licence not read", "asset:data:geo:GSE33463 not downloaded", "asset:data:geo:GSE48149 licence not read", "asset:data:geo:GSE48149 not downloaded", "asset:data:geo:GSE84395 licence not read", "asset:data:geo:GSE84395 not downloaded", "execution.not_run", "method.reader_differs_from_extractor", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The “limma package” in R software (R version 4.2.2) was employed to identify significant DE-FRGs between the two groups using parameters |Log2fold change| >0.5 and adj.P < 0.05 (Table 1)22."], "order_basis": "analysis_only", "id": "assay:1"}]},"meta":{"api_version":"v1","automation_ladder_snapshot":"ba34b49490d9bfaf","automation_ladder_caveats":["Method extraction is rule-based and has no hand-judged accuracy sample. Evidence bands order textual support; method agreement intervals are null (n=0), never probabilities. Work-type validation does not validate methods.","Operation coverage describes only explicitly named methods. It does not prove a complete protocol, compatible parameters, successful compilation or re-execution. Unrecognised or ambiguous methods require review.","Data reachability is a textual accession or public-data statement. No dataset was downloaded or checked for access, completeness or licence.","Capabilities are planning states seeded from the dated equipment register. No bench ownership, completed quote or partner booking is inferred. Procurement counts are dependencies, not guaranteed replications or financial returns.","Tier and blocker counts are deterministic assessments of this corpus, not measured agreement. R3 is reserved for procurement; unresolved methods and data require human work (R4). The most restrictive axis wins, with capability, method, data as tie-breaks. Resolving one blocker can leave another at the same tier."],"engine":"corpus_engine","engine_provider":"rule_based_v1+lex99e1195c7d6f","run_id":"run-20261023T122449232913","source":"esearch","source_detail":"PubMed mirror biomed_pubmed (read-only)","run_status":"complete","imported_at":"2026-11-03T20:16:52+00:00","generated_at":"2026-11-04T01:43:50+00:00","total_papers":5529,"total_assays_tracked":29,"automatable_assay_families":6,"validated":true,"caveat_count":9,"blocking_caveats":5,"caveats":[{"code":"low_evidence_dominates","basis":["4,152 of 5,529 classifications (75.1%) matched no cue evidence and sit at the 0.05 floor.","34 of 230 data_only calls (14.8%) are in that group."],"claim":"Work type and confidence bands","gloss":"Most records were classified from their title and framing alone, with assay-cue coverage at zero.","detail":"Most classifications in this run rest on no matched evidence at all. At the evidence floor the work type comes from title framing and a fixed prior, not from anything in the paper, so the published work-type split should be read as a rough triage signal rather than a measured distribution. The impact is bounded but not removed: since 2026-10-23 the engine excludes floor-confidence records from the shortlist entirely and publishes them separately as held out, so the recommendation is unaffected even though the census is.","status":"unverified","severity":"high"},{"code":"runtime_is_modelled","basis":["Runtime is summed from published per-assay hour figures. Timing awaits the first executed replication.","4,407 of 5,529 records (79.7%) model to 0.00 hours because no assay was matched, which the ranking then reads as \"cheapest\".","0 of 10 shortlisted records carry a 0.00-hour estimate."],"claim":"Estimated replication runtime","gloss":"The hour figures are estimates built from assay mentions; timing awaits the first executed replication.","detail":"The hour figures are a cost model derived from the bench steps implied by each matched assay family. The first executed replication will supply measured timing. Records without an assay match currently receive a zero-hour model value.","status":"unverified","severity":"high"},{"code":"signal_score_without_citations","basis":["5,529 of 5,529 records (100.0%) have no citation count from the source metadata."],"claim":"Signal score","gloss":"The signal score uses publication-type weight because the mirror supplied zero citation counts.","detail":"The source supplied no citation counts for any record in this run, so the signal score is publication-type weight only. The citation term of the ranking is inert here, and the shortlist is ordered by publication type alone.","status":"unverified","severity":"high"},{"code":"abstract_absent","basis":["1,667 of 5,529 records (30.2%) carry no abstract at all."],"claim":"Evidence base for classification","gloss":"Some records contain a title and publication type only, defining the available classification evidence.","detail":"Where the mirror contains a title and publication types only, those fields define the classifier evidence. A methods section is required for fuller automated and human review.","status":"unverified","severity":"medium"},{"code":"classifier_agreement_measured","basis":["Published two-way decision agreement 77.7% (310 of 399 decidable records) from a stratified hand-check of 438 real records.","Three-way diagnostic agreement 73.9% (288 of 390 decidable records).","data_only agreement 75.2% (100 of 133 decidable).","48 record(s) could not be decided from title and abstract and were excluded from the denominator.","56 analysis-only records were published under another work type, so the data_only share is not an upper bound."],"claim":"Published automatable decision","gloss":"This figure has been checked against a human reading the same papers, so we know how often it is right.","detail":"The published automatable / not-automatable distribution comes from a rule-based classifier that was hand-checked against a fixed ground-truth definition. The agreement rates below are measured, not estimated. The three-way work type remains a diagnostic and is reported separately.","status":"measured","severity":"high"},{"code":"llm_comparison_not_measured","basis":["rule_based_v1+lex99e1195c7d6f produced 5,529 of 5,529 classifications.","Measured on 438 labelled records; these are RULE-BASED figures, not model results."],"claim":"Rule-based versus LLM agreement","gloss":"Every number here is the rule engine’s own. Model comparison begins when a working API credential is connected and measured.","detail":"The published two-way decision agreed with a human reading on 310 of 399 decidable records (77.7%). The diagnostic three-way label agreed on 288 of 390 decidable records (73.9%). Every percentage in this run belongs to the rule engine. Model comparison begins when a working API credential is connected and measured.","status":"not_measured","severity":"high"},{"code":"confidence_is_a_band","basis":["low band: 135 of 210 hand-judged records were correct, 64.3% [57.6, 70.5] (95% CI).","medium band: 80 of 104 hand-judged records were correct, 76.9% [68.0, 84.0] (95% CI).","high band: 73 of 76 hand-judged records were correct, 96.0% [89.0, 98.7] (95% CI).","A fitted calibrated probability was built, measured and withdrawn: it overstated held-out accuracy and reorders nothing."],"claim":"Confidence band and evidence score","gloss":"The confidence band carries a measured accuracy interval; 0.85 represents relative rank strength.","detail":"Confidence on this run is a BAND plus its measured accuracy interval. The number stored alongside each record is a monotone evidence score that orders records; 0.85 represents relative rank strength. A fitted probability was withdrawn after it overstated held-out accuracy. Threshold on the band.","status":"measured","severity":"medium"},{"code":"three_way_split_is_diagnostic","basis":["The corpus is published at the two-way decision: automatable or not-automatable. The three-way split (data_only / physical / mixed) is published as a diagnostic with its measured reliability attached, not as a claim, because the rule engine's `mixed` call cannot be defended.","The engine called 22 record(s) mixed; 10 matched the human judgement (45.5%, 95% CI 26.9-65.3).","The engine's `mixed` call is right on 10/22 hand-judged records (45.5%, 95% CI 26.9%-65.3%); a constant `physical` guess scores 36.4%.","The engine's `mixed` call does not reliably beat a constant `physical` guess on the hand-judged records it labels mixed.","The sign of that difference flips between two independent samples, so the three-way refinement is not demonstrably informative.","The mixed stratum contributes a disproportionate share of errors relative to its size.","At title-only depth the physical/mixed split collapses: a title says that work was done, not whether it was also analysed."],"claim":"The published decision is two-way","gloss":"The three-way split is shown for diagnosis only. The published split is two ways.","detail":"Count and filter the corpus on automatable versus not-automatable. The data_only / physical / mixed split remains an internal engine diagnostic because the mixed call has lower measured reliability.","status":"measured","severity":"medium"},{"code":"automatable_flag_unproven","basis":["6 of 29 assay families are marked automatable now: meta_analysis, machine_learning, survival_analysis, pca, molecular_docking, differential_expression.","The flag is a rule over assay family and work type; validation begins with the first executed replication.","It inherits the 75.2% data_only agreement rate, because the flag requires the record to be analysis-only."],"claim":"Automatable now","gloss":"This flag marks assay families the rules classify as data-only. The first executed replication will validate it.","detail":"This flag marks families the rules consider replicable from data alone. Its validity depends on the analysis-only classification and will be measured through an executed replication.","status":"unverified","severity":"medium"}],"confidence_policy":"band_with_measured_interval","confidence_contract":{"policy":"band_with_measured_interval","summary":"Confidence is reported as a BAND with a measured accuracy interval. The raw evidence score supplies monotone ordering. A fitted point estimate was withdrawn after it overstated held-out accuracy.","evidence_score":"A monotone evidence score in [0,1] built from cue mass, axis separation and text coverage. Use it to order records. Interpret 0.85 as relative rank strength.","confidence_band":"low | medium | high. Consumers threshold on this value.","band_accuracy":"Wilson 95% interval on the measured agreement of the hand-judged records in this band.","calibrated_confidence":"WITHDRAWN. Reintroduction requires held-out evidence demonstrating reliable estimates across every bin."},"band_accuracy":{"low":{"n":210,"low":0.576,"band":"low","high":0.7046,"agree":135,"accuracy":0.6429},"high":{"n":76,"low":0.8903,"band":"high","high":0.9865,"agree":73,"accuracy":0.9605},"medium":{"n":104,"low":0.6795,"band":"medium","high":0.8397,"agree":80,"accuracy":0.7692}},"provider":"rule_based_v1+lex99e1195c7d6f","lexicon_id":"99e1195c7d6f","contract_published_at":"2026-10-23T12:25:29.022232+00:00","confidence_is_band":true,"confidence_is_probability":false,"decision_taxonomy":"two_way","decidable_fraction_two_way":0.8854,"decidable_fraction_two_way_low":0.8565,"decidable_fraction_two_way_high":0.9143,"decidable_fraction_three_way":0.8586,"decidable_fraction_three_way_low":0.8287,"decidable_fraction_three_way_high":0.8884,"decidable_population":5529,"validation_sample_size":438,"three_way_cost_fraction":0.0268,"three_way_cost_records":148,"decision_counts":{"automatable":230,"not_automatable":5299},"decision_split":{"automatable":{"count":230,"share":0.04159884246699222},"not_automatable":{"count":5299,"share":0.9584011575330078}},"diagnostic_work_type_counts":{"mixed":24,"physical":5275,"data_only":230}}}