Direct calibration of PICKY-designed microarrays.

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The complete set of microarray experiment results is archived in the GEO database with series accession number GSE14717. Additional data files and Perl programs described in this paper can be obtained from the website http://www.complex.iastate.edu under the PICKY Download area.\nCONCLUSION: PICKY-designed microarray probes are highly reliable over a wide range of hybridization temperatures and sample concentrations. The microarray calibration method reported here allows researchers to experimentally optimize their hybridization conditions. Because this method is straightforward, uses existing microarrays and relatively inexpensive synthesized samples, it can be used by any lab that uses microarrays designed by PICKY. In addition, other microarrays can be reanalyzed by PICKY to obtain the thermodynamically closest nontarget information for calibration.", "claims": [{"id": "claim-data-access", "statement": "The complete set of programs, code libraries and sample data files to illustrate how to select calibration probes based on the PICKY design output and how to harvest the calibration results for analysis can be downloaded from the PICKY website [16]. These files provide both a tutorial of the calibration procedure and sample code that can be modified to work on other data files. See the included README.txt for more details of each supplementary file. 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