Stress associated gene expression in blood cells is related to outcome in radiotherapy treated head and neck cancer patients.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:23009663", "kind": "science-record", "revision": 8, "content_hash": "sha256:31f71d393326396aaa100b95ff7c8c57ff1fd04f61deaf3643d6d3e85973d380", "created_at": "2026-11-01T15:40:02Z", "updated_at": "2026-11-01T22:41:56Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Stress associated gene expression in blood cells is related to outcome in radiotherapy treated head and neck cancer patients.", "doi": "10.1186/1471-2407-12-426", "pmid": "23009663", "authors": ["Siv K Bøhn", "Kjell M Russnes", "Amrit K Sakhi", "Magne Thoresen", "Marit Holden", "Jan Ø Moskaug", "Mari C Myhrstad", "Ole K Olstad", "Sigbjørn Smeland", "Rune Blomhoff"], "journal": "BMC cancer", "year": 2012, "abstract": "BACKGROUND: We previously observed that a radiotherapy-induced biochemical response in plasma was associated with favourable outcome in head and neck squamous carcinoma cancer (HNSCC) patients. The aim of the present study was to compare stress associated blood cell gene expression between two sub-groups of HNSCC patients with different biochemical responses to radiotherapy.\nMETHODS: Out of 87 patients (histologically verified), 10 biochemical 'responders' having a high relative increase in plasma oxidative damage and a concomitant decrease in plasma antioxidants during radiotherapy and 10 'poor-responders' were selected for gene-expression analysis and compared using gene set enrichment analysis.\nRESULTS: There was a significant induction of stress-relevant gene-sets in the responders following radiotherapy compared to the poor-responders. The relevance of the involvement of similar stress associated gene expression for HNSCC cancer and radioresistance was verified using two publicly available data sets of 42 HNSCC cases and 14 controls (GEO GSE6791), and radiation resistant and radiation sensitive HNSCC xenografts (E-GEOD-9716).\nCONCLUSIONS: Radiotherapy induces a systemic stress response, as revealed by induction of stress relevant gene expression in blood cells, which is associated to favourable outcome in a cohort of 87 HNSCC patients. Whether these changes in gene expression reflects a systemic effect or are biomarkers of the tumour micro-environmental status needs further study.\nTRIAL REGISTRATION: Raw data are available at ArrayExpress under accession number E-MEXP-2460.", "claims": [{"id": "claim-method", "statement": "BAMarrayTM, a Bayesian ANOVA method for the analysis of microarray data that adjusts for multiple testing, was used to identify differentially regulated gene transcripts [12].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:23009663", "locator": "sections[6] \"Identification of significantly differentially expressed genes at baseline and during RT\" paragraph 2", "quote": "BAMarrayTM, a Bayesian ANOVA method for the analysis of microarray data that adjusts for multiple testing, was used to identify differentially regulated gene transcripts [12]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw data are available at ArrayExpress by accession number E-MEXP-2460 http://www.ebi.ac.uk/microarray-as/ae/.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:23009663", "locator": "sections[5] \"Data analysis\" paragraph 2", "quote": "The raw data are available at ArrayExpress by accession number E-MEXP-2460 http://www.ebi.ac.uk/microarray-as/ae/."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study used BAMarray, a multiple-testing-adjusted Bayesian ANOVA method, to identify differentially regulated gene transcripts, and the raw microarray data were available through ArrayExpress under accession number E-MEXP-2460.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:23009663", "locator": "sections[6] \"Identification of significantly differentially expressed genes at baseline and during RT\" paragraph 2", "quote": "BAMarrayTM, a Bayesian ANOVA method for the analysis of microarray data that adjusts for multiple testing, was used to identify differentially regulated gene transcripts [12]."}, {"asset": "source:pmid:23009663", "locator": "sections[0] \"Abstract\" paragraph 5", "quote": "Raw data are available at ArrayExpress under accession number E-MEXP-2460."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study used BAMarray, a multiple-testing-adjusted Bayesian ANOVA method, to identify differentially regulated gene transcripts, and the raw microarray data were available through ArrayExpress under accession number E-MEXP-2460.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:23009663"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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