A visual description of the dissection of the cerebral surface vasculature and associated meninges and the choroid plexus from rat brain.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:23183685", "kind": "science-record", "revision": 5, "content_hash": "sha256:3fb7f6bb3cabe6d0624c3897cca1298b72818aa4d6a7991c1a8a1dad55d6ce94", "created_at": "2026-11-01T15:40:02Z", "updated_at": "2026-11-01T18:46:53Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A visual description of the dissection of the cerebral surface vasculature and associated meninges and the choroid plexus from rat brain.", "doi": "10.3791/4285", "pmid": "23183685", "authors": ["John F Bowyer", "Monzy Thomas", "Tucker A Patterson", "Nysia I George", "Jeffrey A Runnells", "Mark S Levi"], "journal": "Journal of visualized experiments : JoVE", "year": 2012, "abstract": "This video presentation was created to show a method of harvesting the two most important highly vascular structures, not residing within the brain proper, that support forebrain function. They are the cerebral surface (superficial) vasculature along with associated meninges (MAV) and the choroid plexus which are necessary for cerebral blood flow and cerebrospinal fluid (CSF) homeostasis. The tissue harvested is suitable for biochemical and physiological analysis, and the MAV has been shown to be sensitive to damage produced by amphetamine and hyperthermia. As well, the major and minor cerebral vasculatures harvested in MAV are of potentially high interest when investigating concussive types of head trauma. The MAV dissected in this presentation consists of the pial and some of the arachnoid membrane (less dura) of the meninges and the major and minor cerebral surface vasculature. The choroid plexus dissected is the structure that resides in the lateral ventricles as described by Oldfield and McKinley. The methods used for harvesting these two tissues also facilitate the harvesting of regional cortical tissue devoid of meninges and larger cerebral surface vasculature, and is compatible with harvesting other brain tissues such as striatum, hypothalamus, hippocampus, etc. The dissection of the two tissues takes from 5 to 10 min total. The gene expression levels for the dissected MAV and choroid plexus, as shown and described in this presentation can be found at GSE23093 (MAV) and GSE29733 (choroid plexus) at the NCBI GEO repository. This data has been, and is being, used to help further understand the functioning of the MAV and choroid plexus and how neurotoxic events such as severe hyperthermia and AMPH adversely affect their function.", "claims": [{"id": "claim-data-access", "statement": "The gene expression levels for the dissected MAV and choroid plexus, as shown and described in this presentation can be found at GSE23093 (MAV) and GSE29733 (choroid plexus) at the NCBI GEO repository.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:23183685", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The gene expression levels for the dissected MAV and choroid plexus, as shown and described in this presentation can be found at GSE23093 (MAV) and GSE29733 (choroid plexus) at the NCBI GEO repository."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using Agilent whole-rat genome oligonucleotide arrays and volcano plots, the study found that MAV gene expression was markedly distinct from striatum and parietal cortex, with the source expression data available through NCBI GEO under GSE23093 and GSE29733.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:23183685", "locator": "sections[6] \"5. Representative Results\" paragraph 3", "quote": "The gene expression of more than 11,000 genes with official NCBI gene symbols in MAV of control animals was compared to those recorded in the striatum and parietal cortex. Over 2000 of these genes had a 2.5-fold or more differing expression in the MAV compared to the two neuronal-rich tissues, and 550 genes had a 10-fold greater difference in expression."}, {"asset": "source:pmid:23183685", "locator": "sections[6] \"5. 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