Long non-coding RNA expression profile in human gastric cancer and its clinical significances.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:24063685", "kind": "science-record", "revision": 9, "content_hash": "sha256:a97290bc7556003c718df10119971ee9b657f03ace31ae0b6d005aa245da7a8b", "created_at": "2026-11-01T15:40:03Z", "updated_at": "2026-11-01T22:41:59Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Long non-coding RNA expression profile in human gastric cancer and its clinical significances.", "doi": "10.1186/1479-5876-11-225", "pmid": "24063685", "authors": ["Haojun Song", "Weiliang Sun", "Guoliang Ye", "Xiaoyun Ding", "Zhong Liu", "Sijie Zhang", "Tian Xia", "Bingxiu Xiao", "Yang Xi", "Junming Guo"], "journal": "Journal of translational medicine", "year": 2013, "abstract": "BACKGROUND: Long non-coding RNAs (lncRNAs) are prevalently transcribed in the genome yet their potential roles in human cancers are not well understood. The aim of the present study was to determine the lncRNA expression profile in gastric cancer and its potential clinical value.\nMETHODS: The global lncRNA expression profile in gastric cancer was measured by lncRNA microarray. Levels of two representative lncRNAs, H19 and uc001lsz, were confirmed by real-time reverse transcriptase-polymerase chain reaction. The relationship between their levels and clinicopathological factors of patients with gastric cancer was explored. A receiver operating characteristic (ROC) curve was constructed for differentiating gastric cancer from benign gastric diseases.\nRESULTS: Total of 135 lncRNAs, which differential expression levels between tumor and non-tumorous tissues were more than twofold, were found (GEO No. GSE47850). The most down-regulated lncRNAs in gastric cancer tissues were FER1L4, uc001lsz, BG491697, AF131784, uc009ycs, BG981369, AF147447, HMlincRNA1600, and AK054588; while the most up-regulated ones were H19, HMlincRNA717, BM709340, BQ213083, AK054978, and DB077273. H19 was found highly expressed in stomach and liver cancer cell lines, while lowly expressed in lung cancer and prostate cancer cell lines. Uc001lsz was lowly expressed in gastric, lung and liver cancer cell lines, while highly expressed in prostate cancer. The areas under ROC curves were up to 0.613, 0.751, and 0.761 for H19, uc001lsz, and the combination, respectively.\nCONCLUSIONS: The lncRNA expression profile in gastric cancer suggests the potential roles of lncRNAs in gastric cancer occurrence and development. The overexpression of H19 in gastric cancer suggests that H19 may be participated in gastric cancer. The reduced expression of uc001lsz in gastric cancer cell lines and tissues, its associations with TNM stage, and its dysregulation in early cancer and precancerous lesions suggest that uc001lsz may be a potential marker for the diagnosis of early gastric cancer.", "claims": [{"id": "claim-method", "statement": "One way analysis of variance test, two-tailed Student’s t-test and rank-sum test were used as appropriate.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:24063685", "locator": "sections[10] \"Statistical analysis\" paragraph 2", "quote": "One way analysis of variance test, two-tailed Student’s t-test and rank-sum test were used as appropriate."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The lncRNA expression patterns between gastric cancer tissues and adjacent non-tumorous tissues were found to be significantly different (Figure 1). Total of 135 lncRNAs, which expression change was more than twofold, were found (GEO accession numbers is 47850; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE47850).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:24063685", "locator": "sections[12] \"LncRNA expression profiles in gastric cancer tissues relative to adjacent non-tumorous tissues\" paragraph 2", "quote": "The lncRNA expression patterns between gastric cancer tissues and adjacent non-tumorous tissues were found to be significantly different (Figure 1). Total of 135 lncRNAs, which expression change was more than twofold, were found (GEO accession numbers is 47850; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE47850)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using receiver operating characteristic analysis of tissue H19 and uc001lsz expression, the study reported areas under the curves of 0.613 for H19, 0.751 for uc001lsz, and 0.761 for their combination.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:24063685", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The global lncRNA expression profile in gastric cancer was measured by lncRNA microarray. Levels of two representative lncRNAs, H19 and uc001lsz, were confirmed by real-time reverse transcriptase-polymerase chain reaction. The relationship between their levels and clinicopathological factors of patients with gastric cancer was explored. A receiver operating characteristic (ROC) curve was constructed for differentiating gastric cancer from benign gastric diseases."}, {"asset": "source:pmid:24063685", "locator": "sections[17] \"Potential diagnostic values of H19 and uc001lsz\" paragraph 2", "quote": "The areas under ROC curves were up to 0.613, 0.751, and 0.761 for H19, uc001lsz, and the combination, respectively (Figure 4)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using receiver operating characteristic analysis of tissue H19 and uc001lsz expression, the study reported areas under the curves of 0.613 for H19, 0.751 for uc001lsz, and 0.761 for their combination.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:24063685"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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