Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP).

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Here, we report isolation of telomeres by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using a transcription activator-like (TAL) protein recognizing telomere repeats. Telomeres recognized by the tagged TAL protein were immunoprecipitated with an antibody against the tag and subjected to identification of telomere-binding molecules. enChIP-mass spectrometry (enChIP-MS) targeting telomeres identified known and novel telomere-binding proteins. The data have been deposited to the ProteomeXchange with identifier PXD000461. In addition, we showed that RNA associated with telomeres could be isolated by enChIP. Identified telomere-binding molecules may play important roles in telomere biology. enChIP using TAL proteins would be a useful tool for biochemical analysis of specific genomic regions of interest.", "claims": [{"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository27 with the dataset identifier PXD000461 and DOI 10.6019/PXD000461. PRIDE accessions are 31251–31256.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:24201379", "locator": "sections[23] \"Acknowledgments\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository27 with the dataset identifier PXD000461 and DOI 10.6019/PXD000461. PRIDE accessions are 31251–31256."}], "state": "complete"}, {"id": "claim-target-result", "statement": "No statistical method or numerical statistical result is reported; the study used enChIP-MS to identify known and novel telomere-binding proteins, and deposited the proteomics data in ProteomeXchange under identifier PXD000461.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:24201379", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "enChIP-mass spectrometry (enChIP-MS) targeting telomeres identified known and novel telomere-binding proteins."}, {"asset": "source:pmid:24201379", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data have been deposited to the ProteomeXchange with identifier PXD000461."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "No statistical method or numerical statistical result is reported; the study used enChIP-MS to identify known and novel telomere-binding proteins, and deposited the proteomics data in ProteomeXchange under identifier PXD000461.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:24201379"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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