Proteomics of secretory and endocytic organelles in Giardia lamblia.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:24732305", "kind": "science-record", "revision": 6, "content_hash": "sha256:f3d0e72b6f1e31a9a3c4722ff0d514fb7b2baef7ec1a3076653953f491b37141", "created_at": "2026-11-01T15:40:05Z", "updated_at": "2026-11-01T21:19:48Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteomics of secretory and endocytic organelles in Giardia lamblia.", "doi": "10.1371/journal.pone.0094089", "pmid": "24732305", "authors": ["Petra B Wampfler", "Vinko Tosevski", "Paolo Nanni", "Cornelia Spycher", "Adrian B Hehl"], "journal": "PloS one", "year": 2014, "abstract": "Giardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocytic organelles termed peripheral vesicles (PVs), mainly on the exposed dorsal side. When trophozoites reach the G2/M restriction point in the cell cycle they can begin another round of cell division or encyst if they encounter specific environmental cues. They induce neogenesis of Golgi-like organelles, encystation-specific vesicles (ESVs), for regulated secretion of cyst wall material. PVs and ESVs are highly simplified and thus evolutionary diverged endocytic and exocytic organelle systems with key roles in proliferation and transmission to a new host, respectively. Both organelle systems physically and functionally intersect at the endoplasmic reticulum (ER) which has catabolic as well as anabolic functions. However, the unusually high degree of sequence divergence in Giardia rapidly exhausts phylogenomic strategies to identify and characterize the molecular underpinnings of these streamlined organelles. To define the first proteome of ESVs and PVs we used a novel strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data. From the limited size datasets we retrieved many hypothetical but also known organelle-specific factors. In contrast to PVs, ESVs appear to maintain a strong physical and functional link to the ER including recruitment of ribosomes to organelle membranes. Overall the data provide further evidence for the formation of a cyst extracellular matrix with minimal complexity. 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"Gl24451", "Hypothetical protein", ""], ["HGS", "Gl87926", "Hypothetical protein", "vd", "HGS", "Gl3920", "Hypothetical protein", ""], ["HGS", "Gl90434", "Hypothetical protein", "", "HGS", "Gl4852", "Hypothetical protein", ""], ["HGS", "Gl113722", "Hypothetical protein", "", "HGS", "Gl5890", "Hypothetical protein", ""], ["HGS", "Gl137705", "Hypothetical protein", "", "HGS", "Gl6334", "Hypothetical protein", ""], ["HGS", "Gl137712", "Hypothetical protein", "", "HGS", "Gl10181", "Hypothetical protein", ""], ["HNS", "Gl5568", "Hypothetical protein", "", "HGS", "Gl10524", "Hypothetical protein", ""], ["HNS", "Gl8799", "Hypothetical protein", "", "HGS", "Gl10608", "Hypothetical protein", ""], ["HNS", "Gl11246", "Hypothetical protein", "", "HGS", "Gl112893", "Hypothetical protein", ""], ["HNS", "Gl13262", "Hypothetical protein", "", "HGS", "Gl14971", "Hypothetical protein", ""], ["HNS", "Gl14345", "Coiled-coil protein", "", "HGS", "Gl16543", "Hypothetical protein", ""], ["HNS", "Gl14877", "Hypothetical protein", "", "HGS", "Gl4018", "Hypothetical protein", ""], ["HNS", "Gl15956", "WD-40 repeat protein", "er", "HGS", "Gl4270", "Hypothetical protein", "pm, cy, pv?"], ["HNS", "Gl22806", "Hypothetical protein", "", "HGS", "Gl17347", "Hypothetical protein", ""], ["HNS", "Gl23357*", "Hypothetical protein", "", "HNS", "Gl91354*", "Hypothetical protein", ""], ["HNS", "Gl88581", "Synaptic glycoprotein SC2", "er", "HNS", "Gl10522", "Hypothetical protein", ""], ["HNS", "Gl94654", "Hypothetical protein", "", "HNS", "Gl16237", "Hypothetical protein", ""], ["MET", "Gl4059", "Methylthioadenosine/S adenosylhomocysteine nucleosidase", "", "HNS", "Gl16367", "Hypothetical protein", ""], ["MET", "Gl4507", "CTP synthase/UTP-ammonia lyase", "", "HNS", "Gl16998", "Hypothetical protein", ""], ["MET", "Gl6757*", "Isochorismatase", "", "HNS", "Gl7778", "Hypothetical protein", ""], ["PRO", "Gl7896", "26S proteasome non-ATPase regulatory subunit 7", "", "R/T", "Gl11287", "Ribosomal protein L7Ae", ""], ["PRO", "Gl16823", "Non ATPase subunit MPR1 of 26S proteasome", "", "R/T", "Gl14869", "Ribosomal protein L24", ""], ["NUC", "Gl7474", "DNA-directed RNA polymerase RPB3", "", "SUG", "Gl104031", "Glycogen synthase, putative", ""], ["R/T", "Gl9899*", "putative eIF2A", "", "NUA", "Gl16328*", "Pseudouridine synthase", ""], ["R/T", "Gl10341*", "putative 50S ribosomal protein L1", "", "MOT", "Gl13825", "Kinesin-1", ""], ["R/T", "Gl10780", "Ribosomal protein S27", "", "TPO", "Gl40224", "Multi drug resistance (MDR) protein-like protein", "er, pe"], ["R/T", "Gl11319", "U3 snRNP IMP3, putative", "", "ER", "Gl14856", "Signal recognition particle (SRP) receptor", ""], ["R/T", "Gl11755*", "30S ribosomal protein S8E", "", "MIS", "Gl3095*", "Cyclin_N domain containing protein", ""], ["R/T", "Gl15156", "Signal recognition particle 54 kDa (SRP54) GTPase", "cy, er, es", "MIS", "Gl8394*", "RdX-domain containing protein", ""], ["R/T", "Gl15546*", "putative eIF3", "", "MIS", "Gl32697*", 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