Discovery of prognostic biomarker candidates of lacunar infarction by quantitative proteomics of microvesicles enriched plasma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:24752076", "kind": "science-record", "revision": 8, "content_hash": "sha256:6121d831bda7bd6eeb6d2a01991f2d73deeec9947b45edf25d135371714c3f13", "created_at": "2026-11-01T15:40:05Z", "updated_at": "2026-11-01T22:42:04Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Discovery of prognostic biomarker candidates of lacunar infarction by quantitative proteomics of microvesicles enriched plasma.", "doi": "10.1371/journal.pone.0094663", "pmid": "24752076", "authors": ["Arnab Datta", "Christopher P Chen", "Siu Kwan Sze"], "journal": "PloS one", "year": 2014, "abstract": "BACKGROUND: Lacunar infarction (LACI) is a subtype of acute ischemic stroke affecting around 25% of all ischemic stroke cases. Despite having an excellent recovery during acute phase, certain LACI patients have poor mid- to long-term prognosis due to the recurrence of vascular events or a decline in cognitive functions. Hence, blood-based biomarkers could be complementary prognostic and research tools.\nMETHODS AND FINDING: Plasma was collected from forty five patients following a non-disabling LACI along with seventeen matched control subjects. The LACI patients were monitored prospectively for up to five years for the occurrence of adverse outcomes and grouped accordingly (i.e., LACI-no adverse outcome, LACI-recurrent vascular event, and LACI-cognitive decline without any recurrence of vascular events). Microvesicles-enriched fractions isolated from the pooled plasma of four groups were profiled by an iTRAQ-guided discovery approach to quantify the differential proteome. The data have been deposited to the ProteomeXchange with identifier PXD000748. Bioinformatics analysis and data mining revealed up-regulation of brain-specific proteins including myelin basic protein, proteins of coagulation cascade (e.g., fibrinogen alpha chain, fibrinogen beta chain) and focal adhesion (e.g., integrin alpha-IIb, talin-1, and filamin-A) while albumin was down-regulated in both groups of patients with adverse outcome.\nCONCLUSION: This data set may offer important insight into the mechanisms of poor prognosis and provide candidate prognostic biomarkers for validation on larger cohort of individual LACI patients.", "claims": [{"id": "claim-method", "statement": "One-way ANOVA followed by post hoc Tukey test was used for scale variables such as age. Nonparametric Kruskal-Wallis H Test was used for comparing ordinal variables such as demographic characteristics and baseline rick factors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:24752076", "locator": "sections[18] \"Statistical Analyses\" paragraph 2", "quote": "One-way ANOVA followed by post hoc Tukey test was used for scale variables such as age. Nonparametric Kruskal-Wallis H Test was used for comparing ordinal variables such as demographic characteristics and baseline rick factors."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [66] with the dataset identifier PXD000748.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:24752076", "locator": "sections[31] \"Raw Data Availability\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [66] with the dataset identifier PXD000748."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using iTRAQ-guided discovery quantitative proteomics of pooled group-wise plasma microvesicle-enriched fractions, the study found up-regulation of brain-specific, coagulation-cascade, and focal-adhesion proteins and down-regulation of albumin in patients with adverse outcomes; the mass-spectrometry data were deposited in ProteomeXchange under identifier PXD000748.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:24752076", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Microvesicles-enriched fractions isolated from the pooled plasma of four groups were profiled by an iTRAQ-guided discovery approach to quantify the differential proteome."}, {"asset": "source:pmid:24752076", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Bioinformatics analysis and data mining revealed up-regulation of brain-specific proteins including myelin basic protein, proteins of coagulation cascade (e.g., fibrinogen alpha chain, fibrinogen beta chain) and focal adhesion (e.g., integrin alpha-IIb, talin-1, and filamin-A) while albumin was down-regulated in both groups of patients with adverse outcome."}, {"asset": "source:pmid:24752076", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The data have been deposited to the ProteomeXchange with identifier PXD000748."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using iTRAQ-guided discovery quantitative proteomics of pooled group-wise plasma microvesicle-enriched fractions, the study found up-regulation of brain-specific, coagulation-cascade, and focal-adhesion proteins and down-regulation of albumin in patients with adverse outcomes; the mass-spectrometry data were deposited in ProteomeXchange under identifier PXD000748.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:24752076"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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