Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:25368622", "kind": "science-record", "revision": 8, "content_hash": "sha256:8e26d10f53f8e163b125b4a2fd111ad810a93f894136795b693ad79366720869", "created_at": "2026-11-01T15:40:06Z", "updated_at": "2026-11-01T22:42:09Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana.", "doi": "10.3389/fpls.2014.00554", "pmid": "25368622", "authors": ["Ines Lassowskat", "Christoph Böttcher", "Lennart Eschen-Lippold", "Dierk Scheel", "Justin Lee"], "journal": "Frontiers in plant science", "year": 2014, "abstract": "Mitogen-activated protein kinases (MAPKs) target a variety of protein substrates to regulate cellular signaling processes in eukaryotes. In plants, the number of identified MAPK substrates that control plant defense responses is still limited. Here, we generated transgenic Arabidopsis thaliana plants with an inducible system to simulate in vivo activation of two stress-activated MAPKs, MPK3, and MPK6. Metabolome analysis revealed that this artificial MPK3/6 activation (without any exposure to pathogens or other stresses) is sufficient to drive the production of major defense-related metabolites, including various camalexin, indole glucosinolate and agmatine derivatives. An accompanying (phospho)proteome analysis led to detection of hundreds of potential phosphoproteins downstream of MPK3/6 activation. Besides known MAPK substrates, many candidates on this list possess typical MAPK-targeted phosphosites and in many cases, the corresponding phosphopeptides were detected by mass spectrometry. Notably, several of these putative phosphoproteins have been reported to be associated with the biosynthesis of antimicrobial defense substances (e.g., WRKY transcription factors and proteins encoded by the genes from the \"PEN\" pathway required for penetration resistance to filamentous pathogens). Thus, this work provides an inventory of candidate phosphoproteins, including putative direct MAPK substrates, for future analysis of MAPK-mediated defense control. (Proteomics data are available with the identifier PXD001252 via ProteomeXchange, http://proteomecentral.proteomexchange.org).", "claims": [{"id": "claim-method", "statement": "The resulting intensity matrix (feature × sample) was log2-transformed and subjected to Two-Way ANOVA.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:25368622", "locator": "sections[14] \"Non-targeted data analysis and metabolite annotation\" paragraph 2", "quote": "The resulting intensity matrix (feature × sample) was log2-transformed and subjected to Two-Way ANOVA."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Dataset: The proteomics data have been deposited to the ProteomeXchange Consortium (Vizcaíno et al., 2014) (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD001252 and DOI 10.6019/PXD001252.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:25368622", "locator": "sections[27] \"Concluding remarks\" paragraph 3", "quote": "Dataset: The proteomics data have been deposited to the ProteomeXchange Consortium (Vizcaíno et al., 2014) (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD001252 and DOI 10.6019/PXD001252."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Artificial MPK3/6 activation was sufficient to drive major defense-metabolite accumulation and detect hundreds of candidate downstream phosphoproteins; the metabolomics used Two-Way ANOVA with a significant genotype–treatment-time interaction at p < 0.01, while the proteomics source data are accessible through ProteomeXchange under identifier PXD001252 via http://proteomecentral.proteomexchange.org.", "kind": "quantitative", "target": {"value": 0.01, "unit": "p-value", "tolerance": null, "source": "Features with a significant genotype-treatment time interaction (p < 0.01) were filtered by intensity (maximum intensity ≥ 214 counts)."}, "evidence": [{"asset": "source:pmid:25368622", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Metabolome analysis revealed that this artificial MPK3/6 activation (without any exposure to pathogens or other stresses) is sufficient to drive the production of major defense-related metabolites, including various camalexin, indole glucosinolate and agmatine derivatives."}, {"asset": "source:pmid:25368622", "locator": "sections[14] \"Non-targeted data analysis and metabolite annotation\" paragraph 2", "quote": "Features with a significant genotype-treatment time interaction (p < 0.01) were filtered by intensity (maximum intensity ≥ 214 counts)."}, {"asset": "source:pmid:25368622", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "(Proteomics data are available with the identifier PXD001252 via ProteomeXchange, http://proteomecentral.proteomexchange.org)"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Artificial MPK3/6 activation was sufficient to drive major defense-metabolite accumulation and detect hundreds of candidate downstream phosphoproteins; the metabolomics used Two-Way ANOVA with a significant genotype–treatment-time interaction at p < 0.01, while the proteomics source data are accessible through ProteomeXchange under identifier PXD001252 via http://proteomecentral.proteomexchange.org.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:25368622"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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