Proteomic analysis and functional characterization of mouse brain mitochondria during aging reveal alterations in energy metabolism.

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Additionally, mechanisms leading to the preservation of mitochondrial function promote longevity. In this study we investigated the proteomic and functional alterations in brain mitochondria isolated from mature (5 months old), old (12 months old), and aged (24 months old) mice as determinants of normal \"healthy\" aging. Here the global changes concomitant with aging in the mitochondrial proteome of mouse brain analyzed by quantitative mass-spectrometry based super-SILAC identified differentially expressed proteins involved in several metabolic pathways including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Despite these changes, the bioenergetic function of these mitochondria was preserved. Overall, this data indicates that proteomic changes during aging may compensate for functional defects aiding in preservation of mitochondrial function. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD001370 (http://proteomecentral.proteomexchange.org/dataset/PXD001370).", "claims": [{"id": "claim-target-result", "statement": "Our data revealed significant age-associated changes in proteins important for mitochondrial energy metabolism; however, we observed that aged mice do not exhibit functional alterations in brain mitochondrial respiration, suggesting that the observed mitochondrial proteomic changes may counteract any functional defects that arise with age.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:25546256", "locator": "sections[0] \"1 Introduction\" paragraph 4", "quote": "Our data revealed significant age-associated changes in proteins important for mitochondrial energy metabolism; however, we observed that aged mice do not exhibit functional alterations in brain mitochondrial respiration, suggesting that the observed mitochondrial proteomic changes may counteract any functional defects that arise with age."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Our data revealed significant age-associated changes in proteins important for mitochondrial energy metabolism; however, we observed that aged mice do not exhibit functional alterations in brain mitochondrial respiration, suggesting that the observed mitochondrial proteomic changes may counteract any functional defects that arise with age.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:25546256"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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