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pmid:25548914

The incidence of sexually dimorphic gene expression varies greatly between tissues in the rat.

Russell D J Huby · Philip Glaves · Richard Jackson

PloS one · 2014 doi:10.1371/journal.pone.0115792

Paper

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Experiment

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Data

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Analysis

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Execution

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2026-11-01T15:40:07Z
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title
The incidence of sexually dimorphic gene expression varies greatly between tissues in the rat.
doi
10.1371/journal.pone.0115792
pmid
25548914
authors array · 3 items
[0]
Russell D J Huby
[1]
Philip Glaves
[2]
Richard Jackson
journal
PloS one
year
2014
abstract
The sexually dimorphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets to be sexually dimorphically expressed (SDE) if they were measurably expressed above background in at least one sex, there was at least a two-fold difference in expression (dimorphism) between the sexes, and the differences were statistically significant after correcting for false discovery. 14.5% of expressed probesets were SDE in at least one tissue, with higher expression nearly twice as prevalent in males compared to females. Most were SDE in a single tissue. Surprisingly, nearly half of the probesets that were (SDE) in multiple tissues were oppositely sex biased in different tissues, and most SDE probesets were also expressed without sex bias in other tissues. Two genes were widely SDE: Xist (female-only) and Eif2s3y (male-only). The frequency of SDE probesets varied widely between tissues, and was highest in the duodenum (6.2%), whilst less than 0.05% in over half of the surveyed tissues. The occurrence of SDE probesets was not strongly correlated between tissues. Within individual tissues, however, relational networks of SDE genes were identified. In the liver, networks relating to differential metabolism between the sexes were seen. The estrogen receptor was implicated in differential gene expression in the duodenum. To conclude, sexually dimorphic gene expression is common, but highly tissue-dependent. Sexually dimorphic gene expression may provide insights into mechanisms underlying phenotypic sex differences. Online data are provided as a resource for further analyses (GEO reference GSE63362).
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Statistical differences between male and female expression values were determined using Student’s 2-tailed T-test, then applying the Benjamin-Hochberg correction for false discovery.
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Statistical differences between male and female expression values were determined using Student’s 2-tailed T-test, then applying the Benjamin-Hochberg correction for false discovery.
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The distribution of SDE probesets across rat tissues.
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Incidence of SDE probesets
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The distribution of SDE probesets across rat tissues.
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SDE in at least 1 tissue
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The incidence of SDE probesets common to pairs of tissues.
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Hierarchical clustering of SDE probesets
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The incidence of SDE probesets common to pairs of tissues.
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30
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31
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6
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14
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27
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Ileum
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4
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Pancreas
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The incidence of SDE probesets by tissue.
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PMC4280129
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12
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Tissue distribution of SDE probesets
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The incidence of SDE probesets by tissue.
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#SDE probesets that are expressed in one tissue, one sex
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221
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234
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112
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164
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ne expression varies greatly between tissues in the rat.
[1]
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[2]
orphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechi
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se no regulated procedures were carried out. Rats were humanely killed at a designated establishmen
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enes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets t
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Complete file 
{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:25548914", "kind": "science-record", "revision": 6, "content_hash": "sha256:b7761d6f6ee35089dbc36cf011a871c1da331d2bb14bb84fd50ae3eac1a50ebe", "created_at": "2026-11-01T15:40:07Z", "updated_at": "2026-11-01T21:19:56Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The incidence of sexually dimorphic gene expression varies greatly between tissues in the rat.", "doi": "10.1371/journal.pone.0115792", "pmid": "25548914", "authors": ["Russell D J Huby", "Philip Glaves", "Richard Jackson"], "journal": "PloS one", "year": 2014, "abstract": "The sexually dimorphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets to be sexually dimorphically expressed (SDE) if they were measurably expressed above background in at least one sex, there was at least a two-fold difference in expression (dimorphism) between the sexes, and the differences were statistically significant after correcting for false discovery. 14.5% of expressed probesets were SDE in at least one tissue, with higher expression nearly twice as prevalent in males compared to females. Most were SDE in a single tissue. Surprisingly, nearly half of the probesets that were (SDE) in multiple tissues were oppositely sex biased in different tissues, and most SDE probesets were also expressed without sex bias in other tissues. Two genes were widely SDE: Xist (female-only) and Eif2s3y (male-only). The frequency of SDE probesets varied widely between tissues, and was highest in the duodenum (6.2%), whilst less than 0.05% in over half of the surveyed tissues. The occurrence of SDE probesets was not strongly correlated between tissues. Within individual tissues, however, relational networks of SDE genes were identified. In the liver, networks relating to differential metabolism between the sexes were seen. The estrogen receptor was implicated in differential gene expression in the duodenum. To conclude, sexually dimorphic gene expression is common, but highly tissue-dependent. Sexually dimorphic gene expression may provide insights into mechanisms underlying phenotypic sex differences. 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