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pmid:25852701
Proteome readjustments in the apoplastic space of Arabidopsis thaliana ggt1 mutant leaves exposed to UV-B radiation.
Anna Rita Trentin · Micaela Pivato · Syed M M Mehdi · Leonard Ebinezer Barnabas · Sabrina Giaretta · Marta Fabrega-Prats · Dinesh Prasad · Giorgio Arrigoni · Antonio Masi
Frontiers in plant science · 2015 doi:10.3389/fpls.2015.00128
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Complete file
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Plasma membranes represent a major target for Reactive Oxygen Species (ROS) generated by this harmful radiation. Oxidative reactions occurring in the apoplastic space are counteracted by antioxidative systems mainly involving ascorbate and, to some extent, glutathione. The occurrence of the latter and its exact role in the extracellular space are not well documented, however. In Arabidopsis thaliana, the gamma-glutamyl transferase isoform (GGT1) bound to the cell wall takes part in the so-called gamma-glutamyl cycle for extracellular glutathione degradation and recovery, and may be implicated in redox sensing and balance. In this work, oxidative conditions were imposed with Ultraviolet-B radiation (UV-B) and studied in redox altered ggt1 mutants. The response of ggt1 knockout Arabidopsis leaves to UV-B radiation was assessed by investigating changes in extracellular glutathione and ascorbate content and their redox state, and in apoplastic protein composition. Our results show that, on UV-B exposure, soluble antioxidants respond to the oxidative conditions in both genotypes. Rearrangements occur in their apoplastic protein composition, suggesting an involvement of Hydrogen Peroxide (H2O2), which may ultimately act as a signal. Other important changes relating to hormonal effects, cell wall remodeling, and redox activities are discussed. We argue that oxidative stress conditions imposed by UV-B and disruption of the gamma-glutamyl cycle result in similar stress-induced responses, to some degree at least. Data are available via ProteomeXchange with identifier PXD001807.", "claims": [{"id": "claim-method", "statement": "After checking for a normal distribution, data were tested with One-Way ANOVA using the General linear models (GLM) procedure in SAS (SAS 9.2, 2008). Data with a non-normal distribution were submitted to a non-parametric test (Kruskal-Wallis) using XLSTAT (2014 version). 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Significance was established at P = 0.05."}, {"asset": "source:pmid:25852701", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD001807."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "UV-B exposure and disruption of the gamma-glutamyl cycle produced similar stress-induced responses in Arabidopsis leaves, with rearrangements in the apoplastic proteome and changes in soluble-antioxidant responses.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:25852701"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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