Effect of Embryo Vitrification on Rabbit Foetal Placenta Proteome during Pregnancy.

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We observed in a previous study that the vitrification procedure for the cryopreservation of embryos introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. Now, we have conducted a proteomic study to determine whether protein alterations in the foetal placenta induced by the vitrification procedure remain during pregnancy. In this study, we used 2D-DIGE and mass spectrometry (MALDI-TOF-TOF and LC-MS/MS analysis) to identify the protein changes during middle and late stages of gestation (Day 14 and Day 24, respectively) in rabbit foetal placenta. We identified 11 differentially expressed proteins at Day 14 and 13 proteins at Day 24. Data are available via ProteomeXchange with identifiers PXD001840 and PXD001836. In addition, we demonstrate the presence of three proteins, serum albumin, isocitrate dehydrogenase 1 [NADP+], and phosphoglycerate mutase 1, which were altered during pregnancy. We demonstrate the existence of changes in foetal placental protein during pregnancy induced by the vitrification procedure, which brings into question whether vitrification effects observed during foetal development could lead to physiological and metabolic disorders in adulthood. This effect, taken together with other effects reported in the literature, suggests that embryo cryopreservation is not neutral.", "claims": [{"id": "claim-method", "statement": "Data relative to foetus, foetal placenta and maternal placenta weight were analysed using a General Linear Model (GLM).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:25915775", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Data relative to foetus, foetal placenta and maternal placenta weight were analysed using a General Linear Model (GLM)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange with identifiers PXD001840 and PXD001836.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:25915775", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifiers PXD001840 and PXD001836."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Vitrification caused persistent proteomic alterations in rabbit foetal placenta during gestation, with 11 proteins altered at Day 14 and 13 at Day 24; 2D-DIGE data were evaluated by ANOVA with FDR adjustment and PLS regression, and the source proteomics data are available through ProteomeXchange under PXD001840 and PXD001836.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:25915775", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We identified 11 differentially expressed proteins at Day 14 and 13 proteins at Day 24."}, {"asset": "source:pmid:25915775", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifiers PXD001840 and PXD001836."}, {"asset": "source:pmid:25915775", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We demonstrate the existence of changes in foetal placental protein during pregnancy induced by the vitrification procedure"}, {"asset": "source:pmid:25915775", "locator": "sections[16] \"Effect of vitrification on foetal placenta proteome at two different times of gestation\" paragraph 3", "quote": "In the case of 14-day-old foetal placenta, 100 spots were considered statistically significant after ANOVA analysis."}, {"asset": "source:pmid:25915775", "locator": "sections[16] \"Effect of vitrification on foetal placenta proteome at two different times of gestation\" paragraph 3", "quote": "From that list, 16 protein spots (8 upregulated and 8 downregulated) were also detected as significant by PLS regression analysis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Vitrification caused persistent proteomic alterations in rabbit foetal placenta during gestation, with 11 proteins altered at Day 14 and 13 at Day 24; 2D-DIGE data were evaluated by ANOVA with FDR adjustment and PLS regression, and the source proteomics data are available through ProteomeXchange under PXD001840 and PXD001836.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:25915775"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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In this study, we used 2D-DIGE and mass spectrometry (MALDI-TOF-TOF and LC-MS/MS analysis) to ide", "IGE and mass spectrometry (MALDI-TOF-TOF and LC-MS/MS analysis) to identify the protein changes du", "iddle of gestation. Now, we have conducted a proteomic study to determine whether protein alteratio", "ion of embryos introduced transcriptomic and proteomic modifications in the rabbit foetal placenta"], "order_basis": "paper_text_order:274", "id": "assay:1"}]}