Proteomic study of different culture medium serum volume fractions on RANKL-dependent RAW264.7 cells differentiating into osteoclasts.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:25969670", "kind": "science-record", "revision": 6, "content_hash": "sha256:69560c71ddd7ae3ef83d5a7e8f2e1f69112257f090021d042ca7478e57c15d38", "created_at": "2026-11-01T15:40:08Z", "updated_at": "2026-11-01T21:20:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteomic study of different culture medium serum volume fractions on RANKL-dependent RAW264.7 cells differentiating into osteoclasts.", "doi": "10.1186/s12953-015-0073-6", "pmid": "25969670", "authors": ["Qi Xiong", "Lihai Zhang", "Lingli Xin", "Yanpan Gao", "Ye Peng", "Peifu Tang", "Wei Ge"], "journal": "Proteome science", "year": 2015, "abstract": "BACKGROUND: Cultivation of osteoclasts is a basic tool for investigating osteolytic bone diseases. Fetal bovine serum (FBS) is the standard supplement used for in vitro cell culture medium. Typically, the serum volume fraction used for osteoclast cultivation is 10%. In this study, we investigated the use of a low serum (1% FBS) model for culturing osteoclasts.\nRESULTS: To confirm the validity of this model for use in osteoclast research, we compared the capacity for osteoclastogenesis and bone resorption of RANKL-induced RAW 264.7 cells cultured in medium supplemented with 10% FBS and 1% FBS. Osteoclasts were successfully generated in medium supplemented with 1% FBS, and exhibited prolonged longevity and similar bone resorbing ability to those generated in medium supplemented with 10% FBS, although the osteoclasts were smaller in size. Proteomics and bioinformatics analyses were performed to assess the suitability of osteoclasts formed in low serum-containing medium for use in research focusing on osteoclast differentiation and function. Our study demonstrated that a total of 100 proteins were differentially expressed in cells cultured in medium containing 1% FBS, of which 29 proteins were upregulated, and 71 proteins were downregulated. Bioinformatics analysis showed that the electron transport chain and oxidative phosphorylation pathways were downregulated obviously; however, the osteoclast signaling pathway was unaffected. The data have been deposited to the ProteomeXchange with identifier PXD001935.\nCONCLUSION: Our study provides clear evidence of the validity of the low serum model for use in studying RANKL-dependent osteoclasts differentiation and bone resorption with the advantage of prolonged survival time.", "claims": [{"id": "claim-method", "statement": "Statistical differences between parametric data sets were assessed using Student’s t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:25969670", "locator": "sections[17] \"Statistical analysis\" paragraph 2", "quote": "Statistical differences between parametric data sets were assessed using Student’s t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data have been deposited to the ProteomeXchange with identifier PXD001935.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:25969670", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The data have been deposited to the ProteomeXchange with identifier PXD001935."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Student’s t-test was used for statistical comparisons of parametric data, and source data were deposited in ProteomeXchange under PXD001935; the headline result was that osteoclasts generated in 1% FBS showed “prolonged longevity and similar bone resorbing ability” to those generated in 10% FBS, although they were smaller.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:25969670", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Osteoclasts were successfully generated in medium supplemented with 1% FBS, and exhibited prolonged longevity and similar bone resorbing ability to those generated in medium supplemented with 10% FBS, although the osteoclasts were smaller in size."}, {"asset": "source:pmid:25969670", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The data have been deposited to the ProteomeXchange with identifier PXD001935."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Student’s t-test was used for statistical comparisons of parametric data, and source data were deposited in ProteomeXchange under PXD001935; the headline result was that osteoclasts generated in 1% FBS showed “prolonged longevity and similar bone resorbing ability” to those generated in 10% FBS, although they were smaller.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:25969670"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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