Mass spectrometry analysis of K63-ubiquitinated targets in response to oxidative stress.

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The chains differ by the lysine residue by which the ubiquitin monomers are linked. We used yeast Saccharomyces cerevisiae subjected to oxidative stress as a model to study K63 ubiquitination. K63 ubiquitinated targets were pulled-down by the K63-TUBE system (Tandem Ubiquitin Binding Entities) and analyzed by SILAC-based mass spectrometry [2]. The data are associated to the research article 'K63 polyubiquitination is a new modulator of the oxidative stress response' [3]. 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