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pmid:26217820

Myristoylation profiling in human cells and zebrafish.

Malgorzata Broncel · Remigiusz A Serwa · Paulina Ciepla · Eberhard Krause · Margaret J Dallman · Anthony I Magee · Edward W Tate

Data in brief · 2015 doi:10.1016/j.dib.2015.06.010

Paper

complete

Experiment

complete

Data

partial

Analysis

partial

Execution

absent

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title
Myristoylation profiling in human cells and zebrafish.
doi
10.1016/j.dib.2015.06.010
pmid
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authors array · 7 items
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Malgorzata Broncel
[1]
Remigiusz A Serwa
[2]
Paulina Ciepla
[3]
Eberhard Krause
[4]
Margaret J Dallman
[5]
Anthony I Magee
[6]
Edward W Tate
journal
Data in brief
year
2015
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Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.
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The false discovery rate was set to 0.01 for peptides, proteins and sites.
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