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pmid:26258047

Transcriptional Profiling of Mycobacterium tuberculosis Replicating in the Human Type II Alveolar Epithelial Cell Line, A549.

Michelle B Ryndak · Krishna K Singh · Zhengyu Peng · Suman Laal

Genomics data · 2015 doi:10.1016/j.gdata.2015.05.026

Paper

complete

Experiment

complete

Data

partial

Analysis

partial

Execution

absent

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title
Transcriptional Profiling of Mycobacterium tuberculosis Replicating in the Human Type II Alveolar Epithelial Cell Line, A549.
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10.1016/j.gdata.2015.05.026
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Michelle B Ryndak
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Krishna K Singh
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Zhengyu Peng
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Suman Laal
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Genomics data
year
2015
abstract
Alveolar epithelial cells outnumber alveolar macrophages by ~500 fold and increasing evidence suggests Mycobacterium tuberculosis may replicate dramatically in these cells during the initial weeks of infecting the lung [1,2]. Here, we report in experimental detail the transcriptional profiling of Mycobacterium tuberculosis replicating at 72 hr post-infection in the human type II alveolar epithelial cell line, A549, as compared to Mycobacterium tuberculosis growing logarithmically in laboratory broth culture [2]. All resulting transcriptional profiling data was deposited to the Gene Expression Omnibus (GEO) database under the accession number GSE58466.
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The intensity ratio data obtained from microarray chips was used to perform Significance Analysis of Microarrays (SAM) with MultiArray Viewer Software on the TMEV website for determination of differentially expressed genes of M. tuberculosis grown in A549 cells compared to 7H9 broth grown M. tuberculosis
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All resulting transcriptional profiling data was deposited to the Gene Expression Omnibus (GEO) database under the accession number GSE58466.
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The intensity ratio data obtained from microarray chips was used to perform Significance Analysis of Microarrays (SAM) with MultiArray Viewer Software on the TMEV website for determination of differentially expressed genes of M. tuberculosis grown in A549 cells compared to 7H9 broth grown M. tuberculosis [8]. Among genes identified by SAM, only genes that showed a > 2 fold change at a false discovery rate of < 2% were considered significantly differentially expressed.
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The intensity ratio data obtained from microarray chips was used to perform Significance Analysis of Microarrays (SAM) with MultiArray Viewer Software on the TMEV website for determination of differentially expressed genes of M. tuberculosis grown in A549 cells compared to 7H9 broth grown M. tuberculosis [8]. Among genes identified by SAM, only genes that showed a > 2 fold change at a false discovery rate of < 2% were considered significantly differentially expressed.
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Transcriptional Profile of Mycobacterium tuberculosis Replicating in Human Type II Alveolar Epithelial A549 cell line
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