A long noncoding RNA AB073614 promotes tumorigenesis and predicts poor prognosis in ovarian cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26299803", "kind": "science-record", "revision": 9, "content_hash": "sha256:f74487f26304ea3787266cd9f975c5998fc479b2efdc8e320d2d98ffb4468a6b", "created_at": "2026-11-01T15:40:10Z", "updated_at": "2026-11-01T22:42:17Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A long noncoding RNA AB073614 promotes tumorigenesis and predicts poor prognosis in ovarian cancer.", "doi": "10.18632/oncotarget.4541", "pmid": "26299803", "authors": ["Zhongping Cheng", "Jing Guo", "Li Chen", "Ning Luo", "Weihong Yang", "Xiaoyan Qu"], "journal": "Oncotarget", "year": 2015, "abstract": "Long noncoding RNA (lncRNA) profiles in ovarian cancer (OC) remain largely unknown. In the present study, we screened AB073614 as a new candidate lncRNA which promotes development of OC, in two independent datasets (GSE18521 and GSE38666) from the Gene Expression Omnibus (GEO). The level of AB073614 was then detected in 75 paired OC tissues and adjacent normal tissues by qRT-PCR. Results showed that AB073614 expression was significantly up-regulated in 85.3% (64/75) cancerous tissues compared with normal counterparts (P < 0.01). Further, the 5-year overall survival (OS) in OC patients with high expression of AB073614 was inferior to that with low expression (17.2 months vs 30.0 months, P = 0.0025). To investigate the functional role of AB073614, AB073614 siRNA was transfected into OC cell lines. Knockdown of AB073614 expression significantly inhibited cell proliferation and invasion, resulted in cell arrest in G1 phase of cell cycle and a dramatic increase of apoptosis, both in HO-8910 and OVCAR3 cells. In vivo experiment also revealed that knockdown AB073614 inhibited OVCAR3 cells proliferation. Finally, western blot assays indicated that lncRNA AB073614 may exert its function by targeting ERK1/2 and AKT-mediated signaling pathway. In conclusion, our study suggests that lncRNA AB073614 acts as a functional oncogene in OC development.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier method and log-rank test were performed for patients' survival analyses.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26299803", "locator": "sections[20] \"Statistical Analysis\" paragraph 2", "quote": "Kaplan-Meier method and log-rank test were performed for patients' survival analyses."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Affymetrix Human Genome U133plus2 Array for the OC and normal tissue were downloaded from the GEO: GSE18521 and GSE38666. GSE18521 consisted of 53 papillary serous ovarian adenocarcinoma and 16 normal tissue", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26299803", "locator": "sections[10] \"Microarray data of OC\" paragraph 2", "quote": "Affymetrix Human Genome U133plus2 Array for the OC and normal tissue were downloaded from the GEO: GSE18521 and GSE38666. 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This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE18521"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE18521"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:26299803", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.18632/oncotarget.4541", "sha256": "sha256:676c5b282ca5d5a2beb2acbb1202f625803e05e6a3bb5797292579e9a4ca26a3", "bytes": 29531, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.18632/oncotarget.4541", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC4694838", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE18521", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18521", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE18521", "version": null, "note": "Affymetrix Human Genome U133plus2 Array for the OC and normal tissue were downloaded from the GEO: GSE18521 and GSE38666. 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Knockdown of AB073614 ex", "14 siRNA was transfected into OC cell lines. Knockdown of AB073614 expression significantly inhibit", "cells. In vivo experiment also revealed that knockdown AB073614 inhibited OVCAR3 cells proliferatio", "te the functional role of AB073614, AB073614 siRNA was transfected into OC cell lines. Knockdow"], "order_basis": "paper_text_order:820", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.69, "evidence": ["ase of cell cycle and a dramatic increase of apoptosis, both in HO-8910 and OVCAR3 cells. 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