Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26306320", "kind": "science-record", "revision": 5, "content_hash": "sha256:2ef8a5943d72899b6431c165f5221fb8087a4d2dff70f86bc67e71b7fe65202b", "created_at": "2026-11-01T15:40:10Z", "updated_at": "2026-11-01T18:47:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi.", "doi": "10.1016/j.dib.2015.07.007", "pmid": "26306320", "authors": ["Maggy T Sikulu", "James Monkman", "Keyur A Dave", "Marcus L Hastie", "Patricia E Dale", "Roger L Kitching", "Gerry F Killeen", "Brian H Kay", "Jeffry J Gorman", "Leon E Hugo"], "journal": "Data in brief", "year": 2015, "abstract": "This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].", "claims": [{"id": "claim-data-access", "statement": "External repository data: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [8] via the PRIDE partner repository with the dataset identifier PXD002153 and 10.6019/PXD002153.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26306320", "locator": "sections[15] \"Supplementary materials\" paragraph 2", "quote": "External repository data: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [8] via the PRIDE partner repository with the dataset identifier PXD002153 and 10.6019/PXD002153."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study investigated age-associated proteomic changes in Anopheles gambiae and Anopheles stephensi using 2D-DIGE and mass spectrometry; no statistical method for the proteomic comparisons is explicitly stated, while the raw mass-spectrometry data are publicly available through ProteomeXchange under identifier PXD002153.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26306320", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging."}, {"asset": "source:pmid:26306320", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS)."}, {"asset": "source:pmid:26306320", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study investigated age-associated proteomic changes in Anopheles gambiae and Anopheles stephensi using 2D-DIGE and mass spectrometry; no statistical method for the proteomic comparisons is explicitly stated, while the raw mass-spectrometry data are publicly available through ProteomeXchange under identifier PXD002153.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26306320"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Identities were determined using CapHPLC LTQ-Orbitrap XL hybrid MS/MS and MASCOT database searching.", "structure": {"pmcid": "PMC4534588", "section_index": 11, "section_title": "Liquid chromatography (LC)–MS/MS analysis", "row_count": 27, "column_count": 10}, "table": {"label": "Table 1", "caption": "Identities of age associated A. stephensi and A. gambiae spots shown in Fig. 1. Identities were determined using CapHPLC LTQ-Orbitrap XL hybrid MS/MS and MASCOT database searching.", "rows": [["Spot #", "Protein name", "Score", "Coverage", "No. of Proteins", "No. of Unique peptides", "No. of PSMs", "No. of AA", "Mw [kDa]", "Calc. pI"], ["641a", "ASTEI05637-PA hypothetical protein", "950.92", "68.02", "1", "13", "68", "172", "19.5", "5.95"], ["", "ASTEI06099-PA hypothetical protein", "311.69", "33.90", "1", "8", "35", "177", "20.4", "5.91"], ["", "ASTEI09076-PA hypothetical protein", "36.01", "14.29", "2", "2", "2", "189", "21.4", "5.73"], ["707a", "ASTE011623-PA hypothetical protein", "37.91", "14.04", "2", "2", "2", "178", "19.3", "8.18"], ["", "ASTEI01010-PA hypothetical protein", "69.26", "27.65", "2", "4", "7", "217", "23.7", "9.20"], ["763a", "ASTEI10910-PA hypothetical protein", "64.46", "21.26", "1", "2", "2", "127", "13.9", "6.38"], ["", "ASTE002628-PA hypothetical protein", "52.76", "18.25", "3", "2", "3", "137", "14.3", "7.84"], ["774a", "ASTEI052400-PA hypothetical protein", "48.74", "21.74", "2", "2", "3", "92", "10.2", "11.18"], ["659a", "ASTEI05637-PA hypothetical protein", "646.94", "55.23", "1", "8", "30", "172", "19.5", "5.95"], ["", "ASTEI09076-PA hypothetical protein", "78.65", "13.23", "2", "2", "2", "189", "21.4", "5.73"], ["", "ASTEI06099-PA hypothetical protein", "73.27", "15.82", "1", "4", "6", "177", "20.4", "5.91"], ["653a", "ASTEI06860-PA hypothetical protein", "387.50", "49.50", "1", "8", "38", "202", "21.8", "8.29"], ["501a", "ASTEI09467-PA hypothetical protein", "514.91", "29.55", "2", "2", "38", "352", "38.1", "6.95"], ["", "ASTEI07484-PA hypothetical protein", "88.05", "16.25", "2", "4", "7", "277", "31.9", "5.86"], ["", "ASTEI06665-PA hypothetical protein", "75.28", "10.84", "1", "3", "5", "286", "30.9", "6.99"], ["", "ASTE001036-PA hypothetical protein", "69.42", "12.94", "2", "4", "4", "309", "34.2", "6.74"], ["", "ASTEI00995-PA hypothetical protein", "41.85", "10.43", "2", "2", "2", "163", "18.5", "8.62"], ["", "ASTE002048-PA hypothetical protein", "41.39", "8.85", "3", "2", "2", "192", "21.9", "8.40"], ["", "ASTE011344-PA hypothetical protein", "42.68", "6.60", "5", "2", "2", "318", "34.6", "6.54"], ["836a", "ASTEI01727-PA hypothetical protein", "70.13", "20.11", "2", "3", "4", "184", "21.1", "4.79"], ["", "ASTEI05226-PA hypothetical protein", "49.86", "8.97", "3", "2", "2", "223", "25.1", "6.21"], ["890b", "AGAP004031-PA Mitochondrial electron transfer flavour protein subunit alpha", "187.85", "24.40", "2", "6", "9", "332", "34.6", "8.57"], ["", "AGAP005627-PC creatine kinase", "48.78", "7.32", "7", "2", "2", "355", "39.7", "6.47"], ["", "AGAP009833-PA Voltage-dependent anion-selective channel protein 2", "39.70", "8.51", "4", "2", "2", "282", "30.7", "8.56"], ["", "AGAP008278-PA Long form D7 salivary protein", "31.25", "6.11", "1", "2", "3", "311", "35.6", "5.90"], ["", "AGAP006936-PB Mitochondrial Cytochrome c1 haem protein", "0.00", "8.08", "4", "2", "2", "297", "32.7", "8.46"]], "row_count": 27, "column_count": 10, "numeric_cells": 243, "header_rows": 1, "locator": "section[11]/table-wrap[1]#t0005"}}], "gaps": ["asset:data:geo:PXD002153 not downloaded", "asset:data:geo:PXD002153 licence not read"]}, "analysis": {"state": "absent", "methods": [], "targets": [], "gaps": ["method", "operations"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "method", "blocked_on": "method:not_determinable", "axes": {"method": {"tier": "R4", "expressible": false, "required_operations": [], "missing_operations": [], "basis": "The reader quoted the method the paper names (not named), but the corpus's method extractor could not map it onto a DSL operation, so the method is published but not expressible."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. 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Here, we have described the techniques", "ages for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry r", "p mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange w"], "order_basis": "paper_text_order:0", "id": "assay:1"}]}