Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation.

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Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. 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Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated thei", "cer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated thei"], "order_basis": "paper_text_order:14", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation.", "able isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass", "1 or control cells were grown and separately cultured in light and heavy-labeled DMEM medium suppleme", "he Subcellular Protein Fractionation Kit for Cultured Cells (Pierce) following the manufacturer׳s"], "order_basis": "paper_text_order:52", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out", "th quantitative proteomic analysis of Snail1-transfected 3T3-L1 and mMSC cells to identify cellular a", "c labeling for mMSC Snail and control stably transfected cells. mMSC cells were grown in DMEM, detach", "replicates in the same experiment. In Snail-transfected 3T3-L1 cells, we identified 3920 proteins in"], "order_basis": "paper_text_order:514", "id": "assay:3"}]}