Proteomic Analysis of One-carbon Metabolism-related Marker in Liver of Rat Offspring.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26342040", "kind": "science-record", "revision": 7, "content_hash": "sha256:2736e7473c05b0c48468fba3eea2180344badc4c5248044463dc71f707cfa9fc", "created_at": "2026-11-01T15:40:10Z", "updated_at": "2026-11-01T21:58:36Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteomic Analysis of One-carbon Metabolism-related Marker in Liver of Rat Offspring.", "doi": "10.1074/mcp.M114.046888", "pmid": "26342040", "authors": ["Young-Ah You", "Ji Hye Lee", "Eun Jin Kwon", "Jae Young Yoo", "Woo-Sung Kwon", "Myung-Geol Pang", "Young Ju Kim"], "journal": "Molecular & cellular proteomics : MCP", "year": 2015, "abstract": "Maternal food intake has a significant effect on the fetal environment, and an inadequate maternal diet may result in intrauterine growth restriction. Intrauterine growth restriction newborn rat pups nursed by normal diet-fed dams exhibited rapid catch-up growth, which plays a critical role in the risk for metabolic and cardiovascular disease in later life. Specifically, one-carbon metabolism in the liver plays a critical role in placental and fetal growth. Impaired functioning of one-carbon metabolism is associated with increased homocysteine levels. In this study, we applied a comprehensive proteomic approach to identify differential expression of proteins related to one-carbon metabolism in the livers of rat offspring as an effect of maternal food restriction during gestation. Data are available via ProteomeXchange with identifier PXD002578. We determined that betaine-homocysteine S-methyltransferase 1, methylenetetrahydrofolate dehydrogenase 1, and ATP synthase subunit beta mitochondrial (ATP5B) expression levels were significantly reduced in the livers of rat offspring exposed to maternal food restriction during gestation compared with in the offspring of rats fed a normal diet (p < 0.05). Moreover, the expression levels of betaine-homocysteine S-methyltransferase 1, methylenetetrahydrofolate dehydrogenase 1, and ATP synthase subunit beta mitochondrial were negatively correlated with serum homocysteine concentration in male offspring exposed to maternal food restriction during gestation and normal diet during lactation. However, in female offspring only expression levels of methylenetetrahydrofolate dehydrogenase 1 were negatively correlated with homocysteine concentration. This study shows that maternal food restriction during late gestation and normal diet during lactation lead to increased homocysteine concentration through disturbance of one-carbon metabolism in the livers of male offspring. This suggests that male offspring have an increased gender-specific susceptibility to disease in later life through fetal programming.", "claims": [{"id": "claim-method", "statement": "We used Student's t test (p < 0.05) to determine which protein spots were differentially abundant (> 10-fold) between the livers of 3-week-old offspring from the AdLib/AdLib and FR groups. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (31) via the PRIDE partner repository with the data set identifier PXD002578.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26342040", "locator": "sections[17] \"Statistical Analysis\" paragraph 2", "quote": "We used Student's t test (p < 0.05) to determine which protein spots were differentially abundant (> 10-fold) between the livers of 3-week-old offspring from the AdLib/AdLib and FR groups."}, {"asset": "source:pmid:26342040", "locator": "sections[13] \"Database Searching\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (31) via the PRIDE partner repository with the data set identifier PXD002578."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (31) via the PRIDE partner repository with the data set identifier PXD002578.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26342040", "locator": "sections[13] \"Database Searching\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (31) via the PRIDE partner repository with the data set identifier PXD002578."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The headline result was that maternal food restriction during late gestation followed by a normal diet during lactation increased serum homocysteine in male offspring by disturbing hepatic one-carbon metabolism. The statistical methods were Student's t test for differential protein abundance, the Wilcoxon rank-sum test for differences between two groups, and Spearman's correlation for protein expression versus serum homocysteine. The mass-spectrometry proteomics data were deposited in ProteomeXchange through the PRIDE partner repository under identifier PXD002578.", "kind": "quantitative", "target": {"value": 10.0, "unit": "fold change", "tolerance": null, "source": "We used Student's t test (p < 0.05) to determine which protein spots were differentially abundant (> 10-fold) between the livers of 3-week-old offspring from the AdLib/AdLib and FR groups."}, "evidence": [{"asset": "source:pmid:26342040", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "This study shows that maternal food restriction during late gestation and normal diet during lactation lead to increased homocysteine concentration through disturbance of one-carbon metabolism in the livers of male offspring."}, {"asset": "source:pmid:26342040", "locator": "sections[17] \"Statistical Analysis\" paragraph 2", "quote": "We used Student's t test (p < 0.05) to determine which protein spots were differentially abundant (> 10-fold) between the livers of 3-week-old offspring from the AdLib/AdLib and FR groups."}, {"asset": "source:pmid:26342040", "locator": "sections[17] \"Statistical Analysis\" paragraph 2", "quote": "We used the Wilcoxon rank-sum test to evaluate individual differences in two groups (p < 0.05)."}, {"asset": "source:pmid:26342040", "locator": "sections[17] \"Statistical Analysis\" paragraph 2", "quote": "Spearman's correlations were calculated to analyze differences between the expressed proteins and serum Hcy concentration."}, {"asset": "source:pmid:26342040", "locator": "sections[13] \"Database Searching\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (31) via the PRIDE partner repository with the data set identifier PXD002578."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The headline result was that maternal food restriction during late gestation followed by a normal diet during lactation increased serum homocysteine in male offspring by disturbing hepatic one-carbon metabolism. The statistical methods were Student's t test for differential protein abundance, the Wilcoxon rank-sum test for differences between two groups, and Spearman's correlation for protein expression versus serum homocysteine. The mass-spectrometry proteomics data were deposited in ProteomeXchange through the PRIDE partner repository under identifier PXD002578.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26342040"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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