An in vivo transcriptome data set of natural antisense transcripts from Plasmodium falciparum clinical isolates.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26484136", "kind": "science-record", "revision": 8, "content_hash": "sha256:14afdcc4d29a6b6b4bc3897dfc19bd9c2114ec13b8462697a4ef9a13228caf75", "created_at": "2026-11-01T15:40:11Z", "updated_at": "2026-11-01T21:58:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "An in vivo transcriptome data set of natural antisense transcripts from Plasmodium falciparum clinical isolates.", "doi": "10.1016/j.gdata.2014.10.010", "pmid": "26484136", "authors": ["Amit Kumar Subudhi", "P A Boopathi", "Shilpi Garg", "Sheetal Middha", "Jyoti Acharya", "Deepak Pakalapati", "Vishal Saxena", "Mohammed Aiyaz", "Harsha B Orekondy", "Raja C Mugasimangalam", "Paramendra Sirohi", "Sanjay K Kochar", "Dhanpat K Kochar", "Ashis Das"], "journal": "Genomics data", "year": 2014, "abstract": "Antisense transcription is pervasive among biological systems and one of the products of antisense transcription is natural antisense transcripts (NATs). Emerging evidences suggest that they are key regulators of gene expression. With the discovery of NATs in Plasmodium falciparum, it has been suggested that these might also be playing regulatory roles in this parasite. However, all the reports describing the diversity of NATs have come from parasites in culture condition except for a recent study published by us. In order to explore the in vivo diversity of NATs in P. falciparum clinical isolates, we performed a whole genome expression profiling using a strand-specific 244 K microarray that contains probes for both sense and antisense transcripts. In this report, we describe the experimental procedure and analysis thereof of the microarray data published recently in Gene Expression Omnibus (GEO) under accession number GSE44921. This published data provide a wealth of information about the prevalence of NATs in P. falciparum clinical isolates from patients with diverse malaria related disease conditions. Supplementary information about the description and interpretation of the data can be found in a recent publication by Subudhi et al. in Experimental Parasitology (2014).", "claims": [{"id": "claim-data-access", "statement": "In this report, we describe the experimental procedure and analysis thereof of the microarray data published recently in Gene Expression Omnibus (GEO) under accession number GSE44921.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26484136", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In this report, we describe the experimental procedure and analysis thereof of the microarray data published recently in Gene Expression Omnibus (GEO) under accession number GSE44921."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study detected 797 natural antisense transcripts in P. falciparum clinical isolates, of which 545 were unique to the study; probe data were converted to gene-level data using the median of raw signal and background intensities for genes represented by multiple probes, and the source data are deposited in GEO under accession GSE44921.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26484136", "locator": "sections[7] \"Data analysis\" paragraph 2", "quote": "A total of 797 NATs were detected in this study of which 545 were found to be unique to the study reported in [9]."}, {"asset": "source:pmid:26484136", "locator": "sections[7] \"Data analysis\" paragraph 2", "quote": "For genes represented with multiple probes, median of raw signal intensity and background signal intensity was considered."}, {"asset": "source:pmid:26484136", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "This published data provide a wealth of information about the prevalence of NATs in P. falciparum clinical isolates from patients with diverse malaria related disease conditions."}, {"asset": "source:pmid:26484136", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In this report, we describe the experimental procedure and analysis thereof of the microarray data published recently in Gene Expression Omnibus (GEO) under accession number GSE44921."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study detected 797 natural antisense transcripts in P. falciparum clinical isolates, of which 545 were unique to the study; probe data were converted to gene-level data using the median of raw signal and background intensities for genes represented by multiple probes, and the source data are deposited in GEO under accession GSE44921.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26484136"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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