Murine heart gene expression during acute Chagasic myocarditis.

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Initial cardiac insult could be a determinant to the posterior development of chronic Chagasic cardiomyopathy, usually after 10 years in only approximately 30% of chronically infected patients. Herein, we characterized the acute gene expression profiling in heart tissue of two strains of mice infected with T. cruzi (tulahuen strain) at 4 weeks and their respective controls. Gene sequence data are available at NCBI under GEO accession number: GSE63847. The output of the genes expression suggests differences in involvement of protein kinase B (AKT), NCAM1, HLA-DRA, and ubiquitin C genes networks. These gene activation differences may correlate with myocardial contractility during the acute infection.", "claims": [{"id": "claim-data-access", "statement": "Gene sequence data are available at NCBI under GEO accession number: GSE63847.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26484182", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Gene sequence data are available at NCBI under GEO accession number: GSE63847."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study characterized acute heart-tissue gene-expression differences between infected Balb/c and C57BL/6J mice, with differences in AKT, NCAM1, HLA-DRA, and ubiquitin C gene networks that may correlate with myocardial contractility during acute infection. No statistical method is stated in the listed sections. The source data are available at NCBI under GEO accession number GSE63847.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26484182", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The output of the genes expression suggests differences in involvement of protein kinase B (AKT), NCAM1, HLA-DRA, and ubiquitin C genes networks."}, {"asset": "source:pmid:26484182", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These gene activation differences may correlate with myocardial contractility during the acute infection."}, {"asset": "source:pmid:26484182", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Gene sequence data are available at NCBI under GEO accession number: GSE63847."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study characterized acute heart-tissue gene-expression differences between infected Balb/c and C57BL/6J mice, with differences in AKT, NCAM1, HLA-DRA, and ubiquitin C gene networks that may correlate with myocardial contractility during acute infection. 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Materials and methods Mice were euthanized at 4 weeks post-infection. Hearts", "performed on the Agilent G3 SurePrint 8 × 60 Mouse Gene Expression One-Color Microarray Platfor"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Print 8 × 60 Mouse Gene Expression One-Color Microarray Platform. 6 mice per experimental group per", "anned on the Nimblegen MS200 High-Resolution Microarray Scanner on the 2-mm setting. Images were pro"], "order_basis": "paper_text_order:1754", "id": "assay:2"}]}