Snai1 represses Nanog to promote embryonic stem cell differentiation.

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Among these, Nanog represents the more ESC specific factor and its repression correlates with the loss of pluripotency and ESC differentiation [9-11]. During ESC early differentiation, many development-associated genes become upregulated and although, in general, much is known about the pluripotency self-renewal circuitry, the molecular events that lead ESCs to exit from pluripotency and begin differentiation are largely unknown. Snai1 is one the most early induced genes during ESC differentiation in vitro and in vivo [12,13]. Here we show that Snai1 is able to directly repress several stemness-associated genes including Nanog. We use a ESC stable-line expressing a inducible Snai1 protein. We here show microarray analysis of embryonic stem cells (ESC) expressing Snail-ER at various time points of induction with 4-OH. Data were deposited in Gene Expression Omnibus (GEO) datasets under reference GSE57854 and here: http://epigenetics.hugef-research.org/data.php.", "claims": [{"id": "claim-data-access", "statement": "Data were deposited in Gene Expression Omnibus (GEO) datasets under reference GSE57854 and here: http://epigenetics.hugef-research.org/data.php.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26484184", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data were deposited in Gene Expression Omnibus (GEO) datasets under reference GSE57854 and here: http://epigenetics.hugef-research.org/data.php."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Snai1 directly represses several stemness-associated genes, including Nanog; the study used microarray analysis with quantile normalization and background subtraction, and the source data were deposited in GEO under GSE57854. No numerical result for the statistical method is stated.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26484184", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Here we show that Snai1 is able to directly repress several stemness-associated genes including Nanog."}, {"asset": "source:pmid:26484184", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We here show microarray analysis of embryonic stem cells (ESC) expressing Snail-ER at various time points of induction with 4-OH."}, {"asset": "source:pmid:26484184", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data were deposited in Gene Expression Omnibus (GEO) datasets under reference GSE57854 and here: http://epigenetics.hugef-research.org/data.php."}, {"asset": "source:pmid:26484184", "locator": "sections[3] \"Experimental design, materials and methods\" paragraph 4", "quote": "Analyzed data were normalized using quantile normalization and background subtraction by default parameters in Genome Studio."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Snai1 directly represses several stemness-associated genes, including Nanog; the study used microarray analysis with quantile normalization and background subtraction, and the source data were deposited in GEO under GSE57854. 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Here we show that Snai1 is able to", "on without stop codon, in frame with cDNA of mouse ERα ligand‐binding region was obtained by PC"], "order_basis": "paper_text_order:848", "id": "assay:1"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["sing a inducible Snai1 protein. We here show microarray analysis of embryonic stem cells (ESC) expre", "trogen following the manufacturing protocol. Microarray analysis was performed as in Ref. [17]. 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