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pmid:26484234
Transcriptomic analyses of maize ys1 and ys3 mutants reveal maize iron homeostasis.
Tomoko Nozoye · Hiromi Nakanishi · Naoko K Nishizawa
Genomics data · 2015 doi:10.1016/j.gdata.2015.05.021
Paper
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Experiment
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Data
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Analysis
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Execution
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Complete file
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Yellow stripe 1 (ys1) and ys3 are recessive mutants of maize (Zea mays L.) that result in symptoms typical of Fe deficiency, i.e., interveinal chlorosis of the leaves. The ys1 mutant is defective in the YS1 transporter and is therefore unable to take up Fe(III)-MAs complexes. While the ys3 mutant has been shown to be defective in MA release, the causative gene has not been identified. The objective of the present work was to identify the genes responsible for the ys1 and ys3 phenotypes, so as to extend our understanding of Fe homeostasis in maize by qRT-PCR. In agreement with previous reports, the expression level of YS1 was decreased in the ys1 mutant. Moreover, we identified that the expression level of a homolog of TOM1 in maize (ZmTOM1) was significantly decreased in the ys3 mutant. Here described the quality control and analysis that were performed on the dataset. The data is publicly available through the GEO database with accession number GSE44557. 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