Association of aging with gene expression profiling in mouse submandibular glands.

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Many older adults experience dysfunction in their salivary glands, for example xerostomia, which is defined as dry mouth resulting from reduced or absent saliva flow (Nagler et al., 2004 [2]). In the present study, we investigated gene expression in submandibular glands of young (8 weeks old) and adult (50 weeks old) mice to analyze association of aging with gene expression profiling in mouse submandibular glands. Whole-genome gene expression profiles were analyzed using an Illumina Sentrix system with Mouse-WG-6 v.2 Expression BeadChips (Illumina). Of the genes screened, 284 showed detection values at a significance level of P < 0.01. Among those, the expression of 94 genes (33%) showed a greater decrease in adult mice as compared to young mice. On the other hand, that of 190 genes (77%) was increased in the adults more than in young mice. The data obtained in this study are publicly available in the Gene Expression Omnibus (GEO) database (accession number GSE66857).", "claims": [{"id": "claim-data-access", "statement": "The data obtained in this study are publicly available in the Gene Expression Omnibus (GEO) database (accession number GSE66857).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26484237", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data obtained in this study are publicly available in the Gene Expression Omnibus (GEO) database (accession number GSE66857)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Whole-genome expression profiling identified 284 genes with detection significance of P < 0.01 in mouse submandibular glands, and the source data were deposited in GEO under accession GSE66857.", "kind": "quantitative", "target": {"value": 0.01, "unit": "p-value", "tolerance": null, "source": "Of the genes screened, 284 showed detection values at a significance level of P < 0.01."}, "evidence": [{"asset": "source:pmid:26484237", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Of the genes screened, 284 showed detection values at a significance level of P < 0.01."}, {"asset": "source:pmid:26484237", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data obtained in this study are publicly available in the Gene Expression Omnibus (GEO) database (accession number GSE66857)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Whole-genome expression profiling identified 284 genes with detection significance of P < 0.01 in mouse submandibular glands, and the source data were deposited in GEO under accession GSE66857.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26484237"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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