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pmid:26484248

The Porphyromonas gingivalis ferric uptake regulator orthologue does not regulate iron homeostasis.

Catherine Butler · Helen Mitchell · Stuart Dashper · Eric Reynolds

Genomics data · 2015 doi:10.1016/j.gdata.2015.05.042

Paper

complete

Experiment

complete

Data

partial

Analysis

complete

Execution

absent

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Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
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pmid:26484248
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paper object · 10 fields
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title
The Porphyromonas gingivalis ferric uptake regulator orthologue does not regulate iron homeostasis.
doi
10.1016/j.gdata.2015.05.042
pmid
26484248
authors array · 4 items
[0]
Catherine Butler
[1]
Helen Mitchell
[2]
Stuart Dashper
[3]
Eric Reynolds
journal
Genomics data
year
2015
abstract
Porphyromonas gingivalis is a Gram-negative anaerobic bacterium that has an absolute requirement for iron which it transports from the host as heme and/or Fe(2 +). Iron transport must be regulated to prevent toxic effects from excess metal in the cell. P. gingivalis has one ferric uptake regulator (Fur) orthologue encoded in its genome called Har, which would be expected to regulate the transport and usage of iron within this bacterium. As a gene regulator, inactivation of Har should result in changes in gene expression of several genes compared to the wild-type. This dataset (GEO accession number GSE37099) provides information on expression levels of genes in P. gingivalis in the absence of Har. Surprisingly, these genes do not relate to iron homeostasis.
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sections[7] "Data normalization and analysis" paragraph 2
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To identify differentially expressed genes, the LIMMA software package was used with a cutoff of P values < 0.05.
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Deposited data can be found here: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE37099.
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Porphyromonas gingivalis 33277 wild-type vs Fur orthologue Har isogenic mutant ECR455
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