Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats.

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Regulation of synaptic proteins within the brain's reward pathway modulates addiction behaviours, the progression of drug addiction and long-term changes in brain structure and function that result from drug use. Therefore, using large scale proteomics studies we aim to identify global protein expression changes within the dorsal striatum, a key brain region involved in the modulation of addiction. We performed LC-MS/MS analyses on rat striatal synaptosomes following 30 days of methamphetamine self-administration (2 hours/day) and 14 days abstinence. We identified a total of 84 differentially-expressed proteins with known roles in neuroprotection, neuroplasticity, cell cytoskeleton, energy regulation and synaptic vesicles. We identify significant expression changes in stress-induced phosphoprotein and tubulin polymerisation-promoting protein, which have not previously been associated with addiction. In addition, we confirm the role of amphiphysin and phosphatidylethanolamine binding protein in addiction. This approach has provided new insight into the effects of methamphetamine self-administration on synaptic protein expression in a key brain region associated with addiction, showing a large set of differentially-expressed proteins that persist into abstinence. 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Therefore, using large scale proteomics studies we aim to identify global protein ex", "rsist into abstinence. The mass spectrometry proteomics data are available via ProteomeXchange with", "o treatments. Synaptosomal proteins used for proteomics analysis were based on sufficient protein yi"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ollowing Methamphetamine Self-Administration in Rats.", "owing Methamphetamine Self-Administration in Rats.", "addiction. We performed LC-MS/MS analyses on rat striatal synaptosomes following 30 days of m", ", New Zealand (2012R34). Male Sprague-Dawley rats (Rattus norvegicus, 300–350 g) were housed i"], "order_basis": "paper_text_order:81", "id": "assay:2"}]}