Combining two-dimensional gel electrophoresis and metabolomic data in support of dry-season survival in the two main species of the malarial mosquito Anopheles gambiae.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26543889", "kind": "science-record", "revision": 4, "content_hash": "sha256:16f56bbe1de898abd365bd8975bc81c6391b9321733c2299b98963eab571bd59", "created_at": "2026-11-01T15:40:13Z", "updated_at": "2026-11-01T21:20:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Combining two-dimensional gel electrophoresis and metabolomic data in support of dry-season survival in the two main species of the malarial mosquito Anopheles gambiae.", "doi": "10.1016/j.dib.2015.08.031", "pmid": "26543889", "authors": ["K Hidalgo", "K Mouline", "W Mamai", "N Foucreau", "K R Dabiré", "A Bouchereau", "F Simard", "D Renault"], "journal": "Data in brief", "year": 2015, "abstract": "In dry savannahs of West-Africa, the malarial mosquitoes of the Anopheles gambiae sensu stricto complex annually survive the harsh desiccating conditions of the dry season. However, the physiological and biochemical mechanisms underlying how these mosquitoes survive such desiccating conditions are still undefined, and controversial. In this context, we provide the first work examining both proteomic and metabolomic changes in the two molecular forms of A. gambiae s.s (M and S forms) experimentally exposed to the rainy and dry season conditions as they experience in the field. Protein abundances of the mosquitoes were measured using a two-dimensional fluorescence difference gel electrophoresis (2D DIGE) coupled with a matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) and tandem mass spectrometry (MS) for protein identification. These assays were conducted by Applied Biomics (http://www.appliedbiomics.com, Applied Biomics, Inc. Hayward, CA, USA), and the mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD000294. The metabolomic analysis was conducted using both Acquity UPLC(®) system (for amino acid identification), and a gas-chromatography-mass spectrometry platform (for sugars identification). Metabolomic fingerprintings were assessed in the University of Rennes 1, UMR CNRS 6553 EcoBio (France). A detailed interpretation of the obtained data can be found in Hidalgo et al. (2014) [1] (Journal of Insect Physiology (2014)).", "claims": [{"id": "claim-method", "statement": "Then, images were subjected to in-gel analysis and cross-gel analysis using DeCyder v6.5 (GE-Healthcare) with a P-value <0.05 according to Student’s t-tests.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26543889", "locator": "sections[12] \"3.4. 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Statistical analyses included Student’s t-tests, multivariate discriminant analyses, MANOVAs, Benjamini–Hochberg adjustment, Kruskal–Wallis tests, and Bonferroni correction. The mass spectrometry proteomics source data are accessible through ProteomeXchange via the PRIDE partner repository under dataset identifier PXD000294.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26543889", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In this context, we provide the first work examining both proteomic and metabolomic changes in the two molecular forms of A. gambiae s.s (M and S forms) experimentally exposed to the rainy and dry season conditions as they experience in the field."}, {"asset": "source:pmid:26543889", "locator": "sections[12] \"3.4. 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Sugars\" paragraph 2", "quote": "Significance of the galactose and glucose concentrations variation among species and rearing conditions was assessed using Kruskal–Wallis tests, followed by a Bonferroni procedure resulting in a decreased threshold of significance from P-value <0.05 to P-value <0.01."}, {"asset": "source:pmid:26543889", "locator": "sections[2] \"table-wrap\" paragraph 1", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD000294."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study provided the first examination of proteomic and metabolomic changes in M and S forms of Anopheles gambiae s.s exposed to rainy- and dry-season field conditions. 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