Hepatocytes respond differently to major dietary trans fatty acid isomers, elaidic acid and trans-vaccenic acid.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26628894", "kind": "science-record", "revision": 6, "content_hash": "sha256:b2f5bcf7bfad5596be72f59edefdb30a145a9e7a20606460746444cb0868d420", "created_at": "2026-11-01T15:40:14Z", "updated_at": "2026-11-01T21:20:18Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Hepatocytes respond differently to major dietary trans fatty acid isomers, elaidic acid and trans-vaccenic acid.", "doi": "10.1186/s12953-015-0084-3", "pmid": "26628894", "authors": ["Toke P Krogager", "Lone Vendel Nielsen", "Derya Kahveci", "Thomas F Dyrlund", "Carsten Scavenius", "Kristian W Sanggaard", "Jan J Enghild"], "journal": "Proteome science", "year": 2015, "abstract": "BACKGROUND: It has been discussed if the adverse health effect associated with the ingestion of trans fatty acids correlates with the food source, as the composition of the isomers varies in different foods. We have investigated the hepatocellular responses to the predominant trans fatty acid isomers in industrially produced partially hydrogenated vegetable oils (elaidic acid) and products of ruminant origin (trans-vaccenic acid).\nRESULTS: The responses of HepG2-SF cells exposed to 100 μM fatty acids during 7 days were examined. Elaidic acid decreased the cellular proliferation rate while trans-vaccenic acid had no effect. Analysis of cellular triacylglycerol fractions showed, that both trans fatty acids were metabolized by HepG2-SF cells, although elaidic acid, to a higher degree than trans-vaccenic, accumulated in the triacylglycerol fraction. Proteome analysis revealed that the overlap of differentially regulated proteins only contained four proteins, suggesting that the two trans fatty acid isomers affect the cells in different ways. The data are available via ProteomeXchange with identifier PXD000760.\nCONCLUSIONS: Our investigations revealed that the hepatocellular response to the two most abundant dietary positional C18:1 trans fatty acid isomers differ substantially. In addition, the results suggest that trans-vaccenic acid does not affect cholesterol metabolism adversely compared to elaidic acid.", "claims": [{"id": "claim-method", "statement": "For comparison of experimental groups with Control equality of variance were tested (F-test) and appropriate two-tailed t-tests were performed.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26628894", "locator": "sections[18] \"Assessment of HepG2-SF growth in fatty acid supplemented medium by CyQuant proliferation assay\" paragraph 2", "quote": "For comparison of experimental groups with Control equality of variance were tested (F-test) and appropriate two-tailed t-tests were performed."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data are available via ProteomeXchange with identifier PXD000760 (http://proteomecentral.proteomexchange.org).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26628894", "locator": "sections[23] \"SILAC data analysis\" paragraph 2", "quote": "The data are available via ProteomeXchange with identifier PXD000760 (http://proteomecentral.proteomexchange.org)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper used SILAC and DIGE proteomic analyses, with fold-change and p-value significance criteria, and deposited the underlying mass-spectrometry proteomics data in ProteomeXchange/PRIDE under identifier PXD000760. The headline result was that: “Our investigations revealed that the hepatocellular response to the two most abundant dietary positional C18:1 trans fatty acid isomers differ substantially.”", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26628894"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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RESULTS: The responses of HepG2-SF cells exposed to 100 μM fatty acids durin", "t both trans fatty acids were metabolized by HepG2-SF cells, although elaidic acid, to a higher", "assay kit were from Invitrogen. Cell culture HepG2-SF cells (HepG2 cells optimized for serum fr", "rom Invitrogen. Cell culture HepG2-SF cells (HepG2 cells optimized for serum free growth, Cell"], "order_basis": "paper_text_order:529", "id": "assay:1"}, {"role": "data_generation", "definition_id": "mass_spectrometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["s were desalted on C18 stage tips (Proxeon). LC-MS/MS of SILAC samples The peptides were separated"], "order_basis": "paper_text_order:6077", "id": "assay:2"}]}