Data for proteomic analysis of ATP-binding proteins and kinase inhibitor target proteins using an ATP probe.

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Thus, it is clearly important to identify and quantify these interactions for understanding basic cellular mechanisms and the pathogenesis of various diseases. We used an ATP competition assay (competition between ATP and acyl-ATP probes) that enabled us to distinguish specific ATP-binding proteins from non-specific proteins (Adachi et al., 2014) [1]. As a result, we identified 539 proteins, including 178 novel ATP-binding protein candidates. We also established an ATPome selectivity profiling method for kinase inhibitors using our cataloged ATPome list. Normally only kinome selectivity is profiled in selectivity profiling of kinase inhibitors. In this data, we expand the profiled targets from the kinome to the ATPome through performance of ATPome selectivity profiling and obtained target profiles of staurosporine and (S)-crizotinib. The data accompanying the manuscript on this approach (Adachi et al., 2014) [1] have been deposited to the ProteomeXchange with identifier PXD001200.", "claims": [{"id": "claim-data-access", "statement": "The data accompanying the manuscript on this approach (Adachi et al., 2014) [1] have been deposited to the ProteomeXchange with identifier PXD001200.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26693503", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data accompanying the manuscript on this approach (Adachi et al., 2014) [1] have been deposited to the ProteomeXchange with identifier PXD001200."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 539 proteins, including 178 novel ATP-binding protein candidates, using ATP competition assay with MaxQuant/Andromeda analysis and label-free quantitation filters; the associated data were deposited in ProteomeXchange under identifier PXD001200.", "kind": "quantitative", "target": {"value": 80.0, "unit": "per cent", "tolerance": null, "source": "We removed peptides that were inhibited by <80% at 10 μM ATP in order to extract ATP-competitive peptides/sites from label-free quantitation data."}, "evidence": [{"asset": "source:pmid:26693503", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "As a result, we identified 539 proteins, including 178 novel ATP-binding protein candidates."}, {"asset": "source:pmid:26693503", "locator": "sections[10] \"Data analysis\" paragraph 2", "quote": "All raw files were processed by MaxQuant software suite (version 1.3.0.5) supported by the Andromeda search engine for peptide identification [4]."}, {"asset": "source:pmid:26693503", "locator": "sections[10] \"Data analysis\" paragraph 2", "quote": "We removed peptides that were inhibited by <80% at 10 μM ATP in order to extract ATP-competitive peptides/sites from label-free quantitation data."}, {"asset": "source:pmid:26693503", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data accompanying the manuscript on this approach (Adachi et al., 2014) [1] have been deposited to the ProteomeXchange with identifier PXD001200."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 539 proteins, including 178 novel ATP-binding protein candidates, using ATP competition assay with MaxQuant/Andromeda analysis and label-free quantitation filters; the associated data were deposited in ProteomeXchange under identifier PXD001200.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26693503"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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