The dynamic interactome of human Aha1 upon Y223 phosphorylation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26693507", "kind": "science-record", "revision": 8, "content_hash": "sha256:a4ddb9b9b36a2a0cbb491ed95fbcec84fdc43dddc89ae9d5288d7848ff6734fb", "created_at": "2026-11-01T15:40:14Z", "updated_at": "2026-11-01T22:42:29Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The dynamic interactome of human Aha1 upon Y223 phosphorylation.", "doi": "10.1016/j.dib.2015.10.028", "pmid": "26693507", "authors": ["Donald Wolfgeher", "Diana M Dunn", "Mark R Woodford", "Dimitra Bourboulia", "Gennady Bratslavsky", "Mehdi Mollapour", "Stephen J Kron", "Andrew W Truman"], "journal": "Data in brief", "year": 2015, "abstract": "Heat Shock Protein 90 (Hsp90) is an essential chaperone that supports the function of a wide range of signaling molecules. Hsp90 binds to a suite of co-chaperone proteins that regulate Hsp90 function through alteration of intrinsic ATPase activity. Several studies have determined Aha1 to be an important co-chaperone whose binding to Hsp90 is modulated by phosphorylation, acetylation and SUMOylation of Hsp90 [1], [2]. In this study, we applied quantitative affinity-purification mass spectrometry (AP-MS) proteomics to understand how phosphorylation of hAha1 at Y223 altered global client/co-chaperone interaction [3]. Specifically, we characterized and compared the interactomes of Aha1-Y223F (phospho-mutant form) and Aha1-Y223E (phospho-mimic form). We identified 99 statistically significant interactors of hAha1, a high proportion of which (84%) demonstrated preferential binding to the phospho-mimic form of hAha1. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [4] with the dataset identifier PXD001737.", "claims": [{"id": "claim-method", "statement": "An ANOVA test was then performed to identify proteins that indicate significant variability (P-value<0.05) between biological replicates within each group.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26693507", "locator": "sections[8] \"LC–MS/MS data analysis, statistical analysis\" paragraph 4", "quote": "An ANOVA test was then performed to identify proteins that indicate significant variability (P-value<0.05) between biological replicates within each group."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [4] with the dataset identifier PXD001737.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26693507", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [4] with the dataset identifier PXD001737."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using the R statistical package with ANOVA testing and data deposited through ProteomeXchange via the PRIDE partner repository under PXD001737, the study identified 99 statistically significant hAha1 interactors, 84% of which preferentially bound the phospho-mimic form of hAha1.", "kind": "quantitative", "target": {"value": 84.0, "unit": "per cent", "tolerance": null, "source": "We identified 99 statistically significant interactors of hAha1, a high proportion of which (84%) demonstrated preferential binding to the phospho-mimic form of hAha1."}, "evidence": [{"asset": "source:pmid:26693507", "locator": "sections[8] \"LC–MS/MS data analysis, statistical analysis\" paragraph 4", "quote": "Further statistical analysis was performed using the R statistical package (http://www.r-project.org/)."}, {"asset": "source:pmid:26693507", "locator": "sections[8] \"LC–MS/MS data analysis, statistical analysis\" paragraph 4", "quote": "An ANOVA test was then performed to identify proteins that indicate significant variability (P-value<0.05) between biological replicates within each group."}, {"asset": "source:pmid:26693507", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [4] with the dataset identifier PXD001737."}, {"asset": "source:pmid:26693507", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We identified 99 statistically significant interactors of hAha1, a high proportion of which (84%) demonstrated preferential binding to the phospho-mimic form of hAha1."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using the R statistical package with ANOVA testing and data deposited through ProteomeXchange via the PRIDE partner repository under PXD001737, the study identified 99 statistically significant hAha1 interactors, 84% of which preferentially bound the phospho-mimic form of hAha1.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26693507"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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The mass spectrometry proteomics data have been deposited to the P", "inity-purification mass spectrometry (AP-MS) proteomics to understand how phosphorylation of hAha1 a", "biological replicates within each group. The mass spectrometry proteomic data have been deposited to the Pr"], "order_basis": "paper_text_order:502", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Experimental design, materials and methods HEK293 cell lines expressing either hAha1–Y223F–FLA", "taining 5% CO2. 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