← All .science files
pmid:26697320
Microarray analyses during early and later stages of the Arabidopsis/Piriformospora indica interaction.
Khabat Vahabi · Irena Sherameti · Madhunita Bakshi · Anna Mrozinska · Anatoli Ludwig · Ralf Oelmüller
Genomics data · 2015 doi:10.1016/j.gdata.2015.07.019
Paper
complete
Experiment
complete
Data
partial
Analysis
partial
Execution
absent
Execution evidence has not yet been added to this .science file. Explore its methods, data and open questions to plan the next step.
Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
paper object · 10 fields
authors array · 6 items
claims array · 3 items
[0] object · 6 fields
evidence array · 1 item
[0] object · 3 fields
[1] object · 6 fields
gaps array · 0 items
experiment object · 8 fields
steps array · 3 items
[0] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
[1] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
[2] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
materials array · 0 items
instruments array · 0 items
reagents array · 0 items
conditions object · 0 fields
gaps array · 0 items
data object · 3 fields
assets array · 2 items
[0] object · 15 fields
structure object · 4 fields
[1] object · 15 fields
structure object · 15 fields
sample_accessions array · 4 items
gaps array · 2 items
analysis object · 4 fields
methods array · 1 item
[0] object · 10 fields
dsl_operations array · 0 items
missing_operations array · 0 items
parameters object · 0 fields
data_shapes array · 0 items
targets array · 0 items
gaps array · 1 item
capability object · 3 fields
requirements array · 1 item
[0] object · 6 fields
assay_families array · 9 items
gaps array · 0 items
readiness object · 8 fields
axes object · 3 fields
method object · 5 fields
required_operations array · 0 items
missing_operations array · 0 items
capability object · 4 fields
data object · 6 fields
accessions array · 1 item
resolved_accessions array · 1 item
statements array · 1 item
can_run object · 3 fields
dsl_operations array · 0 items
assets array · 2 items
steps array · 1 item
must_build object · 5 fields
dsl_operations array · 0 items
converters array · 0 items
readers array · 0 items
capabilities array · 0 items
data_gaps array · 1 item
gaps array · 1 item
review object · 5 fields
machine_critique object · 5 fields
reader object · 5 fields
evidence_check object · 2 fields
usage object · 3 fields
stages array · 1 item
[0] object · 4 fields
gaps array · 3 items
provenance object · 7 fields
entities array · 3 items
activities array · 3 items
[0] object · 4 fields
used array · 3 items
[0] object · 3 fields
[1] object · 3 fields
[2] object · 3 fields
[1] object · 4 fields
agents array · 2 items
lineage object · 4 fields
gaps array · 7 items
assays array · 1 item
[0] object · 11 fields
required_operations array · 0 items
source_hashes object · 1 field
evidence array · 3 items
Complete file
{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26697320", "kind": "science-record", "revision": 6, "content_hash": "sha256:f359aee19d773c64ee5cb035e25a6c354533278f348c59178397c04ee7aa13f2", "created_at": "2026-11-01T15:40:14Z", "updated_at": "2026-11-01T21:20:19Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Microarray analyses during early and later stages of the Arabidopsis/Piriformospora indica interaction.", "doi": "10.1016/j.gdata.2015.07.019", "pmid": "26697320", "authors": ["Khabat Vahabi", "Irena Sherameti", "Madhunita Bakshi", "Anna Mrozinska", "Anatoli Ludwig", "Ralf Oelmüller"], "journal": "Genomics data", "year": 2015, "abstract": "Colonization of the roots of different plant species by Piriformospora indica results in better plant performance and biotic and abiotic stress tolerance. An increase of the biomass and seed yield is other beneficial effect of P. indica for the host plants. The interaction of P. indica with Arabidopsis thaliana roots is a unique model system to study symbiotic relationships. We describe a co-cultivation system which allows us to investigate the effects of fungal exudates on the root transcriptome before and after the establishment of a physical contact, and during early phases of root colonization. We present a detailed protocol which facilitates easy reproduction of the results (NCBI GEO accession number GSE58771) published by Vahabi et al. (2015) in BMC Plant Biology [1].", "claims": [{"id": "claim-method", "statement": "ROBIN (http://mapman.gabipd.org/web/ guest/robin-download) and MapMan [5] programs were used for analysis of hybridization signal data followed by statistical analysis with the t-test (Fig. 3).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697320", "locator": "sections[8] \"Microarray analysis, data processing\" paragraph 2", "quote": "ROBIN (http://mapman.gabipd.org/web/ guest/robin-download) and MapMan [5] programs were used for analysis of hybridization signal data followed by statistical analysis with the t-test (Fig. 3)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw and normalized data have been submitted to GEO (http://www.ncbi.nlm.nih.gov/geo, submission number GSE58771) [1].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697320", "locator": "sections[8] \"Microarray analysis, data processing\" paragraph 2", "quote": "The raw and normalized data have been submitted to GEO (http://www.ncbi.nlm.nih.gov/geo, submission number GSE58771) [1]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper used a t-test for statistical analysis and submitted the raw and normalized microarray data to GEO under accession GSE58771.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26697320", "locator": "sections[8] \"Microarray analysis, data processing\" paragraph 2", "quote": "ROBIN (http://mapman.gabipd.org/web/ guest/robin-download) and MapMan [5] programs were used for analysis of hybridization signal data followed by statistical analysis with the t-test (Fig. 3)."}, {"asset": "source:pmid:26697320", "locator": "sections[8] \"Microarray analysis, data processing\" paragraph 2", "quote": "The raw and normalized data have been submitted to GEO (http://www.ncbi.nlm.nih.gov/geo, submission number GSE58771) [1]."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper used a t-test for statistical analysis and submitted the raw and normalized microarray data to GEO under accession GSE58771.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26697320"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE58771"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE58771"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:26697320", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1016/j.gdata.2015.07.019", "sha256": "sha256:6a29b485e3e82d2998d8a00bdb9076ecd1ca6f7cca7e45f7161aabd3d91050c6", "bytes": 7258, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1016/j.gdata.2015.07.019", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC4664658", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE58771", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58771", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE58771", "version": null, "note": "The raw and normalized data have been submitted to GEO (http://www.ncbi.nlm.nih.gov/geo, submission number GSE58771) [1].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The raw and normalized data have been submitted to GEO (http://www.ncbi.nlm.nih.gov/geo, submission number GSE58771) [1].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM1419228", "GSM1419229", "GSM1419230", "GSM1419231"], "sample_count": 4, "group_sizes": null, "group_sizes_basis": null, "platform": "198", "taxon": "Arabidopsis thaliana", "title": "Expression data from Arabidopsis thaliana root and piriformaspora indica during log and short term interaction", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE58771 not downloaded", "asset:data:geo:GSE58771 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": null, "specific": null, "label": "t-test", "dsl_operations": [], "missing_operations": [], "expressible": null, "parameters": {}, "data_shapes": [], "evidence": {"score": 1.0, "band": "verbatim", "quote": "ROBIN (http://mapman.gabipd.org/web/ guest/robin-download) and MapMan [5] programs were used for analysis of hybridization signal data followed by statistical analysis with the t-test (Fig. 3)."}}], "targets": [], "gaps": ["method.reader_named_unmapped"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "method", "blocked_on": "method:not_determinable", "axes": {"method": {"tier": "R4", "expressible": false, "required_operations": [], "missing_operations": [], "basis": "The reader quoted the method the paper names (t-test), but the corpus's method extractor could not map it onto a DSL operation, so the method is published but not expressible."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE58771"], "resolved_accessions": ["GSE58771"], "statements": ["The raw and normalized data have been submitted to GEO (http://www.ncbi.nlm.nih.gov/geo, submission number GSE58771) [1]."], "basis": "The paper names GSE58771 in NCBI Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:26697320", "data:geo:GSE58771"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE58771"]}, "gaps": ["method:not_determinable"]}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:26:31Z", "independence": "same_provider"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 8, "usage": {"prompt_tokens": 20852, "completion_tokens": 2971, "cached_tokens": 1568}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:26697320", "type": "paper", "label": "pmid:26697320"}, {"id": "source:pmid:26697320", "type": "manuscript", "label": "PMID 26697320 full text"}, {"id": "data:geo:GSE58771", "type": "dataset", "label": "GEO GSE58771"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:26697320", "type": "paper", "label": "pmid:26697320"}, {"id": "source:pmid:26697320", "type": "manuscript", "label": "PMID 26697320 full text"}, {"id": "data:geo:GSE58771", "type": "dataset", "label": "GEO GSE58771"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-26697320", "type": "extraction", "used": ["source:pmid:26697320"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE58771", "type": "accession_resolution", "used": ["data:geo:GSE58771"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:26697320", "parent_hash": "sha256:91b5d5fd96f8fd82f43e57b76a5a9c3542324cb9798c658ca211e8846bc47c31", "fork_of": null, "generation": 5}, "gaps": ["asset:data:geo:GSE58771 licence not read", "asset:data:geo:GSE58771 not downloaded", "execution.not_run", "method.reader_named_unmapped", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Microarray analyses during early and later stages of th", "Experimental design, materials and methods Microarray analysis, data processing RNA was extracted", "idized to the Arabidopsis Genome Array ATH1 (Affymetrix, USA) at the Kompetenzzentrum für Fluoreszen"], "order_basis": "paper_text_order:0", "id": "assay:1"}]}