miRNA profiling in autism spectrum disorder in China.

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Evidence suggested that the neuropathology of ASD is widely distributed, involving epigenetic regulation in the brain. MiRNAs are a group of endogenous non-coding RNAs that play a critical role in neurodevelopment, neuroplasticity, and other fundamental neurobiological processes. To study miRNA profiling in Autism spectrum disorder in China, we performed miRNA microarray followed quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Here, we describe detailed methods and analysis on these microarray data which has been deposited in Gene Expression Omnibus (GEO): GSE67979.", "claims": [{"id": "claim-data-access", "statement": "The deposited data can be found at: http://www.ncbi.nlm.nih.gov/pubmed/?term=GSE67979.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697346", "locator": "sections[1] \"Direct link to deposited data\" paragraph 2", "quote": "The deposited data can be found at: http://www.ncbi.nlm.nih.gov/pubmed/?term=GSE67979."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper reports miRNA profiling by microarray followed by qRT-PCR, with microarray analysis performed in R and qRT-PCR differences calculated using the 2 − ΔΔCt method; several autism-related aberrantly expressed miRNAs were discovered. 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