Tissue-specific patterns of gene expression in the epithelium and stroma of normal colon in healthy individuals in an aspirin intervention trial.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26697360", "kind": "science-record", "revision": 6, "content_hash": "sha256:c2ed0d79c540ae22a9e8bd1d7635ee0fbaa41364a8290feb03a8e145b22bcebc", "created_at": "2026-11-01T15:40:15Z", "updated_at": "2026-11-01T21:20:21Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Tissue-specific patterns of gene expression in the epithelium and stroma of normal colon in healthy individuals in an aspirin intervention trial.", "doi": "10.1016/j.gdata.2015.08.029", "pmid": "26697360", "authors": ["Sushma S Thomas", "Karen W Makar", "Lin Li", "Yingye Zheng", "Peiying Yang", "Lisa Levy", "Rebecca Y Rudolph", "Paul D Lampe", "Min Yan", "Sanford D Markowitz", "Jeannette Bigler", "Johanna W Lampe", "John D Potter"], "journal": "Genomics data", "year": 2015, "abstract": "Regular aspirin use reduces colon adenoma and carcinoma incidence. UDP-glucuronosyltransferases (UGT) are involved in aspirin metabolism and clearance, and variant alleles in UGT1A6 have been shown to alter salicylic acid metabolism and risk of colon neoplasia. In a randomized, cross-over, placebo-controlled trial of 44 healthy men and women, homozygous for UGT1A6*1 or UGT1A6*2, we explored differences between global epithelial and stromal expression, using Affymetrix U133 + 2.0 microarrays and tested effects of 60-day aspirin supplementation (325 mg/d) on epithelial and stromal gene expression and colon prostaglandin E2 (PGE2) levels. We conducted a comprehensive study of differential gene expression between normal human colonic epithelium and stroma from healthy individuals. Although no statistically significant differences in gene expression were observed in response to aspirin or UGT1A6 genotype, we have identified the genes uniquely and reproducibly expressed in each tissue type and have analyzed the biologic processes they represent. Here we describe in detail how the data, deposited in the Gene Expression Omnibus (GEO) - accession number GSE71571 - was generated including the basic analysis as contained in the manuscript published in BMC Medical Genetics with the PMID 25927723 (Thomas et al., 2015 [9]).", "claims": [{"id": "claim-method", "statement": "Differential expression between interventions within each tissue type and between two UGT1A6 genotypes (*1/*1 and *2/*2) were also examined by using the same paired t-test and the generalized estimating equation approach. Microarray data are publicly available in Gene Expression Omnibus (GEO) with the accession number GSE71571.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697360", "locator": "sections[10] \"Data analysis–microarray analysis\" paragraph 2", "quote": "Differential expression between interventions within each tissue type and between two UGT1A6 genotypes (*1/*1 and *2/*2) were also examined by using the same paired t-test and the generalized estimating equation approach."}, {"asset": "source:pmid:26697360", "locator": "sections[7] \"RNA extraction and microarray procedures\" paragraph 2", "quote": "Microarray data are publicly available in Gene Expression Omnibus (GEO) with the accession number GSE71571."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Microarray data are publicly available in Gene Expression Omnibus (GEO) with the accession number GSE71571.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697360", "locator": "sections[7] \"RNA extraction and microarray procedures\" paragraph 2", "quote": "Microarray data are publicly available in Gene Expression Omnibus (GEO) with the accession number GSE71571."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study found no statistically significant differences in gene expression in response to aspirin or UGT1A6 genotype, and the data were deposited in GEO under accession number GSE71571.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26697360", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Although no statistically significant differences in gene expression were observed in response to aspirin or UGT1A6 genotype, we have identified the genes uniquely and reproducibly expressed in each tissue type and have analyzed the biologic processes they represent."}, {"asset": "source:pmid:26697360", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Here we describe in detail how the data, deposited in the Gene Expression Omnibus (GEO) – accession number GSE71571 – was generated including the basic analysis as contained in the manuscript published in BMC Medical Genetics with the PMID 25927723 (Thomas et al., 2015 [9])."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study found no statistically significant differences in gene expression in response to aspirin or UGT1A6 genotype, and the data were deposited in GEO under accession number GSE71571.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26697360"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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In a randomized, cross-over, placebo-controlled trial of 44 healthy men and women, homozygous for", "olon neoplasia. In a randomized, cross-over, placebo-controlled trial of 44 healthy men and women, homozygou", "stromal tissue. Study design The study was a randomized, double-blind crossover clinical trial designed to examine the effects of aspirin o", "iagnostics, Seattle, WA). Because this was a randomized crossover trial, each person served as his/her own control,"], "order_basis": "paper_text_order:368", "id": "assay:1"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["bal epithelial and stromal expression, using Affymetrix U133 + 2.0 microarrays and tested effects of"], "order_basis": "paper_text_order:563", "id": "assay:2"}]}