Assessment of microRNA expression in mouse epididymal epithelial cells and spermatozoa by next generation sequencing.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26697376", "kind": "science-record", "revision": 8, "content_hash": "sha256:714cbaa534f1d7e523e9acc5905e95c933279d441440d9570fd4638d57bffd9d", "created_at": "2026-11-01T15:40:15Z", "updated_at": "2026-11-01T22:42:31Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Assessment of microRNA expression in mouse epididymal epithelial cells and spermatozoa by next generation sequencing.", "doi": "10.1016/j.gdata.2015.09.012", "pmid": "26697376", "authors": ["Amanda L Anderson", "Simone J Stanger", "Bettina P Mihalas", "Sonika Tyagi", "Janet E Holt", "Eileen A McLaughlin", "Brett Nixon"], "journal": "Genomics data", "year": 2015, "abstract": "The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display a remarkable level of regionalized secretory and absorptive activity. The luminal environment created by this combined secretory and absorptive activity is directly responsible for promoting the functional maturation of spermatozoa and their maintenance in a quiescent and viable state prior to ejaculation. This study was designed to identify the complement of microRNAs (miRNAs) that are expressed within the mouse epididymal epithelial cells and the maturing populations of spermatozoa. Through the use of Next Generation Sequencing technology we have demonstrated that both epididymal epithelial cells and spermatozoa harbour a complex repertoire of miRNAs that have substantially different expression profiles along the length of the tract. These data, deposited in the Gene Expression Omnibus (GEO) with the accession numbers GSE70197 and GSE70198, afford valuable insight into the post-transcriptional control of gene expression within the epididymis and provide the first evidence for the dynamic transformation of the miRNA content of maturing sperm cells. Ultimately such information promises to inform our understanding of the aetiology of male infertility. Herein we provide a detailed description of the methodology used to generate these important data.", "claims": [{"id": "claim-method", "statement": "Differential miRNA expression analysis was undertaken using R script based on, limma and voom libraries (http://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697376", "locator": "sections[9] \"miRNA Next-Generation Sequencing.\" paragraph 3", "quote": "Differential miRNA expression analysis was undertaken using R script based on, limma and voom libraries (http://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus [8] and are accessible through GEO Series accession number GSE70197 (epididymal tissue and epithelial cells miRNAs) and GSE70198 (epididymal spermatozoa miRNAs) (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70197; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70198).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697376", "locator": "sections[9] \"miRNA Next-Generation Sequencing.\" paragraph 3", "quote": "The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus [8] and are accessible through GEO Series accession number GSE70197 (epididymal tissue and epithelial cells miRNAs) and GSE70198 (epididymal spermatozoa miRNAs) (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70197; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70198)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study demonstrated that epididymal epithelial cells and spermatozoa contain complex miRNA repertoires with substantially different expression profiles along the epididymal tract. For the registered target, differential miRNA expression was analyzed using R scripts based on the limma and voom libraries, and the source data were deposited in GEO under accession numbers GSE70197 and GSE70198.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26697376", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Through the use of Next Generation Sequencing technology we have demonstrated that both epididymal epithelial cells and spermatozoa harbour a complex repertoire of miRNAs that have substantially different expression profiles along the length of the tract."}, {"asset": "source:pmid:26697376", "locator": "sections[9] \"miRNA Next-Generation Sequencing.\" paragraph 3", "quote": "Differential miRNA expression analysis was undertaken using R script based on, limma and voom libraries (http://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf)."}, {"asset": "source:pmid:26697376", "locator": "sections[9] \"miRNA Next-Generation Sequencing.\" paragraph 3", "quote": "The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus [8] and are accessible through GEO Series accession number GSE70197 (epididymal tissue and epithelial cells miRNAs) and GSE70198 (epididymal spermatozoa miRNAs) (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70197; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70198)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study demonstrated that epididymal epithelial cells and spermatozoa contain complex miRNA repertoires with substantially different expression profiles along the epididymal tract. 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Inbred Swiss mice were obtained from a breeding colony held at the", "the recommendations prescribed by the ACEC. Mice were housed under a controlled lighting regime (1"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "amplicon_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["each sample and processed for Illumina HiSeq Next-Generation Sequencing for small non-coding miRNAs. The expression"], "order_basis": "paper_text_order:1966", "id": "assay:2"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ected miRNAs was then validated using TaqMan Real-Time PCR (Fig. 1). 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