Genome-wide expression analysis comparing hypertrophic changes in normal and dysferlinopathy mice.

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Our study shows that blocking this pathway in dysferlin deficient mice results in early improvement in histopathology but ultimately accelerates muscle degeneration. Hence, benefits of this approach should be weighed against these potential detrimental effects. Here, we present detailed experimental methods and analysis for the gene expression profiling described in our recently published study in Human Molecular Genetics (Lee et al., 2015). Our data sets have been deposited in the Gene Expression Omnibus (GEO) database (GSE62945) and are available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62945. Our data provide a resource for exploring molecular mechanisms that are related to hypertrophy-induced, accelerated muscular degeneration in dysferlinopathy.", "claims": [{"id": "claim-method", "statement": "The one-way analysis of variance (ANOVA) was used to determine the magnitude (fold change) and statistical significance (p-value) of genes' changes in expression level between sample classes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697388", "locator": "sections[8] \"Data processing\" paragraph 3", "quote": "The one-way analysis of variance (ANOVA) was used to determine the magnitude (fold change) and statistical significance (p-value) of genes' changes in expression level between sample classes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Our data sets have been deposited in the Gene Expression Omnibus (GEO) database (GSE62945) and are available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62945.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26697388", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Our data sets have been deposited in the Gene Expression Omnibus (GEO) database (GSE62945) and are available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62945."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper reports that blocking myostatin signaling in dysferlin-deficient mice initially improves histopathology but ultimately accelerates muscle degeneration. The statistical method used was one-way ANOVA, and the source data are available through GEO accession GSE62945.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26697388", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Our study shows that blocking this pathway in dysferlin deficient mice results in early improvement in histopathology but ultimately accelerates muscle degeneration."}, {"asset": "source:pmid:26697388", "locator": "sections[8] \"Data processing\" paragraph 3", "quote": "The one-way analysis of variance (ANOVA) was used to determine the magnitude (fold change) and statistical significance (p-value) of genes' changes in expression level between sample classes."}, {"asset": "source:pmid:26697388", "locator": "sections[2] \"Direct link to deposited data\" paragraph 2", "quote": "Raw and processed microarray data is available in GEO under accession GSE62945 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62945."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper reports that blocking myostatin signaling in dysferlin-deficient mice initially improves histopathology but ultimately accelerates muscle degeneration. 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