MS-analysis of SILAC-labeled MYC-driven B lymphoma cells overexpressing miR-17-19b.

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Although the role of miR-17-19b (a truncated version of miR-17-92 cluster) is well documented in MYC-driven B cell lymphomagenesis, little is known about the function of the cluster in the maintenance of full-blown lymphomas. We employed SILAC-based quantitative proteomics to identify miR-17-19b targets upon a mild overexpression of the cluster in B cell lymphomas, established from λ-MYC transgenic mice. The proteomics data described in detail in this study, whose follow up analysis with MaxQuant algorithm is part of the recent publication (Mihailovich et al., 2015) [1], are deposited to the ProteomeXchange Consortium via the PRIDE partner repository, with the accession code PRIDE: PXD002810.", "claims": [{"id": "claim-data-access", "statement": "The proteomics data described in detail in this study, whose follow up analysis with MaxQuant algorithm is part of the recent publication (Mihailovich et al., 2015) [1], are deposited to the ProteomeXchange Consortium via the PRIDE partner repository, with the accession code PRIDE: PXD002810.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26977435", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The proteomics data described in detail in this study, whose follow up analysis with MaxQuant algorithm is part of the recent publication (Mihailovich et al., 2015) [1], are deposited to the ProteomeXchange Consortium via the PRIDE partner repository, with the accession code PRIDE: PXD002810."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The proteomics data were analyzed with the MaxQuant algorithm and are accessible through ProteomeXchange via the PRIDE partner repository under accession PRIDE: PXD002810.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26977435", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The proteomics data described in detail in this study, whose follow up analysis with MaxQuant algorithm is part of the recent publication (Mihailovich et al., 2015) [1], are deposited to the ProteomeXchange Consortium via the PRIDE partner repository, with the accession code PRIDE: PXD002810."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The proteomics data were analyzed with the MaxQuant algorithm and are accessible through ProteomeXchange via the PRIDE partner repository under accession PRIDE: PXD002810.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26977435"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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