Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26977438", "kind": "science-record", "revision": 2, "content_hash": "sha256:a9360520a108e827ab5f1e916a22b4903e0726d742c66dbd90b0d51d33311a1e", "created_at": "2026-11-01T15:40:16Z", "updated_at": "2026-11-01T18:48:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant.", "doi": "10.1016/j.dib.2016.02.046", "pmid": "26977438", "authors": ["Lina Zhang", "Marita de Waard", "Hester Verheijen", "Sjef Boeren", "Jos A Hageman", "Toon van Hooijdonk", "Jacques Vervoort", "Johannes B van Goudoever", "Kasper Hettinga"], "journal": "Data in brief", "year": 2016, "abstract": "Here we provide data from shot-gun proteomics, using filtered-aided sample preparation (FASP), dimethyl labeling and LC-MS/MS, to quantify the changes in the repertoire of human milk proteins over lactation. Milk serum proteins were analyzed at week 1, 2, 3 4, 8, 16, and 24 in milk from four individual mothers. A total of 247 proteins were identified, of which 200 proteins were quantified. The data supplied in this article supports the accompanying publication (Zhang et al., 2006) [1]. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (Vizcaíno et al., 2016) [2] via the PRIDE partner repository with the dataset identifier PXD003465.", "claims": [{"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PXD003465.\n\nAn Excel document containing raw as well as filtered and annotated output from MaxQuant.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26977438", "locator": "sections[12] \"Appendix A. Supporting information\" paragraphs 1-3", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PXD003465.\n\nAn Excel document containing raw as well as filtered and annotated output from MaxQuant."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper states no numerical result for the registered target.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26977438"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD003465"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD003465"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:26977438", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1016/j.dib.2016.02.046", "sha256": "sha256:3aeafc6b01ffb5d272fba2b94d85c8d509452ce6aa2c02c8121a7a23069591b8", "bytes": 9773, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1016/j.dib.2016.02.046", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC4781965", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD003465", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD003465", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD003465", "version": null, "note": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PXD003465.\n\nAn Excel document containing raw as well as filtered and annotated output from MaxQuant.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PXD003465.\n\nAn Excel document containing raw as well as filtered and annotated output from MaxQuant.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "Changes over lactation in breast milk serum proteins.", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:PXD003465 not downloaded", "asset:data:geo:PXD003465 licence not read"]}, "analysis": {"state": "absent", "methods": [], "targets": [], "gaps": ["method", "operations"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "method", "blocked_on": "method:not_determinable", "axes": {"method": {"tier": "R4", "expressible": false, "required_operations": [], "missing_operations": [], "basis": "The reader quoted the method the paper names (not named), but the corpus's method extractor could not map it onto a DSL operation, so the method is published but not expressible."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["PXD003465"], "resolved_accessions": ["PXD003465"], "statements": ["The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PXD003465.\n\nAn Excel document containing raw as well as filtered and annotated output from MaxQuant."], "basis": "The paper names PXD003465 in ProteomeXchange Consortium (PRIDE partner repository) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:26977438", "data:geo:PXD003465"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:PXD003465"]}, "gaps": ["method:not_determinable"]}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:27:30Z", "independence": "same_provider"}, "evidence_check": {"method": null, "availability": "verbatim"}, "calls": 8, "usage": {"prompt_tokens": 24785, "completion_tokens": 2289, "cached_tokens": 7184}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:26977438", "type": "paper", "label": "pmid:26977438"}, {"id": "source:pmid:26977438", "type": "manuscript", "label": "PMID 26977438 full text"}, {"id": "data:geo:PXD003465", "type": "dataset", "label": "GEO PXD003465"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:26977438", "type": "paper", "label": "pmid:26977438"}, {"id": "source:pmid:26977438", "type": "manuscript", "label": "PMID 26977438 full text"}, {"id": "data:geo:PXD003465", "type": "dataset", "label": "GEO PXD003465"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-26977438", "type": "extraction", "used": ["source:pmid:26977438"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-PXD003465", "type": "accession_resolution", "used": ["data:geo:PXD003465"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:26977438", "parent_hash": "sha256:4bd571bcc4d99a75d5e7a206d3620b02247da8850e70265d10d717a54a20c49e", "fork_of": null, "generation": 1}, "gaps": ["asset:data:geo:PXD003465 licence not read", "asset:data:geo:PXD003465 not downloaded", "execution.not_run", "method", "operations", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "mass_spectrometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ng publication (Zhang et al., 2006) [1]. The mass spectrometry proteomics data have been deposited to the P", "le preparation (FASP), dimethyl labeling and LC-MS/MS, to quantify the changes in the repertoire o", "Here we provide data from shot-gun proteomics, using filtered-aided sample preparation (FA", "ang et al., 2006) [1]. The mass spectrometry proteomics data have been deposited to the ProteomeXcha"], "order_basis": "paper_text_order:126", "id": "assay:1"}]}