Mapping transcriptome profiles of in vitro iPSC-derived cardiac differentiation to in utero heart development.

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Both in utero (using embryonic heart tissues) and in vitro (using iPSCs and differentiated cells) microarray datasets were deposited to the NCBI Gene Expression Omnibus (GEO) database. The deposited data in GEO include raw microarray data, metadata for sample source information, experimental design, sample and data processing, and gene expression matrix. The data are available under GEO Access Number GSE69317 (GSE69315 for tissue sample microarray data, GSE69316 for iPSCs microarray data, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE69317).", "claims": [{"id": "claim-method", "statement": "The differential analysis was performed using the LIMMA method [5].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26981387", "locator": "sections[3] \"Experimental design, materials and methods\" paragraph 5", "quote": "The differential analysis was performed using the LIMMA method [5]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data are available under GEO Access Number GSE69317 (GSE69315 for tissue sample microarray data, GSE69316 for iPSCs microarray data, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE69317).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26981387", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data are available under GEO Access Number GSE69317 (GSE69315 for tissue sample microarray data, GSE69316 for iPSCs microarray data, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE69317)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study normalized microarray data with RMA, performed differential analysis with LIMMA, and deposited the source data in the NCBI Gene Expression Omnibus under GEO Accession GSE69317.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:26981387", "locator": "sections[3] \"Experimental design, materials and methods\" paragraph 5", "quote": "Microarray data was normalized and processed using the RMA method [3], [4] to calculate the gene expression matrix. The differential analysis was performed using the LIMMA method [5]."}, {"asset": "source:pmid:26981387", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Both in utero (using embryonic heart tissues) and in vitro (using iPSCs and differentiated cells) microarray datasets were deposited to the NCBI Gene Expression Omnibus (GEO) database."}, {"asset": "source:pmid:26981387", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data are available under GEO Access Number GSE69317 (GSE69315 for tissue sample microarray data, GSE69316 for iPSCs microarray data, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE69317)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The study normalized microarray data with RMA, performed differential analysis with LIMMA, and deposited the source data in the NCBI Gene Expression Omnibus under GEO Accession GSE69317.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:26981387"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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