Microarray analysis of kiwifruit (Actinidia chinensis) bark following challenge by the sucking insect Hemiberlesia lataniae (Hemiptera: Diaspididae).

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:26981426", "kind": "science-record", "revision": 8, "content_hash": "sha256:0c67f2f4fbd06b404b9b71292876c47d266b102032f45e419e02beca3e3e4a90", "created_at": "2026-11-01T15:40:17Z", "updated_at": "2026-11-01T22:42:37Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Microarray analysis of kiwifruit (Actinidia chinensis) bark following challenge by the sucking insect Hemiberlesia lataniae (Hemiptera: Diaspididae).", "doi": "10.1016/j.gdata.2016.01.016", "pmid": "26981426", "authors": ["M Garry Hill", "Kirstin V Wurms", "Marcus W Davy", "Elaine Gould", "Andrew Allan", "Nicola A Mauchline", "Zhiwei Luo", "Annette Ah Chee", "Kate Stannard", "Roy D Storey", "Erik H Rikkerink"], "journal": "Genomics data", "year": 2016, "abstract": "Both commercial and experimental genotypes of kiwifruit (Actinidia spp.) exhibit large differences in response to insect pests. An understanding of the vine's physiological response to insect feeding and its genetic basis will be important in assisting the development of varieties with acceptable levels of pest resistance. This experiment describes transcriptome changes observed in the bark of kiwifruit 2 and 7 days after the commencement of feeding by the armored scale insect pest, Hemiberlesia lataniae. Using a cDNA microarray consisting of 17,512 unigenes, we measured transcriptome changes and analyzed these into functional ontology categories using MapMan. Results are available in the GEO database GSE73922 and are described fully in Ref. Hill et al. (2015) [1]. After 7 days, transcripts associated with photosynthesis were down-regulated and secondary metabolism was up-regulated. Differential expression of transcripts associated with stress response was consistent with a defense response involving both effector and herbivore-triggered immunities, with predominant involvement of the salicylic acid phytohormonal pathway. This hypothesis was supported by the results of two laboratory experiments. The methods described here could be further adapted and applied to the study of plant responses to a wide range of sessile sucking pests.", "claims": [{"id": "claim-method", "statement": "Genepix intensity files were preprocessed and analyzed using the limma [7] analysis package in Bioconductor [8]. Data were normalized using the print tip LOESS method [9], and candidate differentially expressed genes (DEGs) identified using a false discovery rate control set at P < 0.05 [10].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:26981426", "locator": "sections[8] \"Gene expression and data analysis\" paragraph 2", "quote": "Genepix intensity files were preprocessed and analyzed using the limma [7] analysis package in Bioconductor [8]. Data were normalized using the print tip LOESS method [9], and candidate differentially expressed genes (DEGs) identified using a false discovery rate control set at P < 0.05 [10]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Results are available in the GEO database GSE73922 and are described fully in Ref. Hill et al. 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Data were normalized using the print tip LOESS method [9], and candidate differentially expressed genes (DEGs) identified using a false discovery rate control set at P < 0.05 [10]."}, "evidence": [{"asset": "source:pmid:26981426", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "After 7 days, transcripts associated with photosynthesis were down-regulated and secondary metabolism was up-regulated."}, {"asset": "source:pmid:26981426", "locator": "sections[8] \"Gene expression and data analysis\" paragraph 2", "quote": "Genepix intensity files were preprocessed and analyzed using the limma [7] analysis package in Bioconductor [8]. 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