Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression.

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Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). AGO2 bound miRNAs were profiled by RNA-seq.", "claims": [{"id": "claim-data-access", "statement": "Data is within this article. mRNA data are available though Gene Expression Omnibus repository (GEO, accession number GEO: GSE37212 ).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27054165", "locator": "sections[1] \"Specifications table\" paragraph 2", "quote": "Data is within this article. mRNA data are available though Gene Expression Omnibus repository (GEO, accession number GEO: GSE37212 )."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper identifies miR-21 targets by immunopurifying AGO2-containing RISC complexes and profiling associated mRNAs by microarray and bound miRNAs by RNA-seq; no statistical method is stated, and the mRNA source data are available through GEO under accession GSE37212.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27054165", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1])."}, {"asset": "source:pmid:27054165", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). 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