Transcriptional profiling of macrophage and tumor cell interactions in vitro.

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To understand the molecular basis of the tumor-supporting role of macrophages in aggressive breast cancer we co-cultured human peripheral monocytes with two breast cancer cell lines representing distinct aggressive cellular phenotype and transcriptionally profiled the changes occurring in both cells during in vitro activated crosstalk. Here we provide a detailed description of the experimental design, sample identity and analysis of the Illumina RNA-Seq data, which have been deposited into Gene Expression Omnibus (GEO): GSE75130.", "claims": [{"id": "claim-method", "statement": "To identify genes differentially expressed between groups we used an algorithm based on Ref. 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