← All .science files
pmid:27274532
Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution.
Irina Boggs · Brad Hine · Grant Smolenksi · Kasper Hettinga · Lina Zhang · Thomas T Wheeler
Data in brief · 2016 doi:10.1016/j.dib.2016.05.013
Paper
complete
Experiment
partial
Data
partial
Analysis
absent
Execution
absent
Execution evidence has not yet been added to this .science file. Explore its methods, data and open questions to plan the next step.
Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
paper object · 10 fields
authors array · 6 items
claims array · 1 item
gaps array · 0 items
experiment object · 8 fields
steps array · 3 items
[0] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
[1] object · 13 fields
parameters object · 0 fields
inputs array · 0 items
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
[2] object · 13 fields
parameters object · 0 fields
inputs array · 0 items
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
materials array · 0 items
instruments array · 0 items
reagents array · 0 items
conditions object · 0 fields
gaps array · 1 item
data object · 3 fields
assets array · 2 items
[0] object · 15 fields
structure object · 4 fields
[1] object · 16 fields
structure object · 5 fields
table object · 8 fields
rows array · 9 items
[0] array · 2 items
[1] array · 2 items
[2] array · 2 items
[3] array · 2 items
[4] array · 2 items
[5] array · 2 items
[6] array · 2 items
[7] array · 2 items
[8] array · 2 items
gaps array · 0 items
capability object · 3 fields
requirements array · 1 item
[0] object · 6 fields
assay_families array · 9 items
gaps array · 0 items
readiness object · 8 fields
axes object · 3 fields
method object · 5 fields
required_operations array · 0 items
missing_operations array · 0 items
capability object · 4 fields
data object · 6 fields
accessions array · 1 item
resolved_accessions array · 0 items
statements array · 1 item
can_run object · 3 fields
dsl_operations array · 0 items
assets array · 2 items
steps array · 1 item
must_build object · 5 fields
dsl_operations array · 0 items
converters array · 0 items
readers array · 0 items
capabilities array · 0 items
data_gaps array · 1 item
gaps array · 1 item
review object · 5 fields
machine_critique object · 5 fields
reader object · 5 fields
evidence_check object · 2 fields
usage object · 3 fields
stages array · 1 item
[0] object · 4 fields
gaps array · 3 items
provenance object · 7 fields
entities array · 3 items
activities array · 3 items
[0] object · 4 fields
used array · 3 items
[0] object · 3 fields
[1] object · 3 fields
[2] object · 3 fields
[1] object · 4 fields
agents array · 2 items
lineage object · 4 fields
gaps array · 7 items
assays array · 1 item
[0] object · 11 fields
required_operations array · 0 items
source_hashes object · 1 field
evidence array · 3 items
Complete file
{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27274532", "kind": "science-record", "revision": 3, "content_hash": "sha256:5a26209c0a473beeab52158f692ffec6258e2ed371a89bb469eb77bd21834b65", "created_at": "2026-11-01T15:40:18Z", "updated_at": "2026-11-01T18:48:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution.", "doi": "10.1016/j.dib.2016.05.013", "pmid": "27274532", "authors": ["Irina Boggs", "Brad Hine", "Grant Smolenksi", "Kasper Hettinga", "Lina Zhang", "Thomas T Wheeler"], "journal": "Data in brief", "year": 2016, "abstract": "Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication \"Changes in the repertoire of bovine milk proteins during mammary involution\" (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011.", "claims": [{"id": "claim-data-access", "statement": "Data is available within this article and through ProteomeXchange accession numbers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27274532", "locator": "sections[1] \"p\" paragraph 1", "quote": "Data is available within this article and through ProteomeXchange accession numbers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The study quantified changes in the bovine milk proteome after drying off using dimethyl isotopic labelling followed by Orbitrap MS/MS, identifying 161 proteins by DML, 51 by GeLC, and 45 spots by MALDI-TOF; the source data are available via ProteomeXchange identifiers PXD003110 and PXD003011.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27274532"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:obtain_source_data blocked on data:accession_absent"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:27274532", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1016/j.dib.2016.05.013", "sha256": "sha256:5e6c5e986f478fe2cbbead2598cf2c7569388610d4babe5a7e79b8a3c5053fc8", "bytes": 9835, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1016/j.dib.2016.05.013", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC4885146", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "asset:table:pmid:27274532:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:582e93a33b4d7e9590a312c39261302e2192112c7d0055b4a2698166d3e1a530", "bytes": 1563, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[1]/table-wrap[1]#t0005", "version": null, "note": null, "structure": {"pmcid": "PMC4885146", "section_index": 1, "section_title": "p", "row_count": 9, "column_count": 2}, "table": {"label": null, "caption": null, "rows": [["Subject area", "Biology"], ["More specific subject area", "Mammary gland physiology"], ["Type of data", "Excel files"], ["How data was acquired", "Two-dimensional electrophoresis followed by PSD MALDI-TOF (LIFT) mass spectrometry of selected spots using a Bruker Ultraflex instrument. One-dimensional gel electrophoresis followed by LC-tandem MS of peptides excised from gel slices (GeLC) using a QStar instrument. Dimethyl labelling followed by LC tandem MS mass spectrometry (DML) using an Oribtrap intrument."], ["Data format", "Raw"], ["Experimental factors", "Skim milk and whey from day 0 and day 3 or day 8 after drying off"], ["Experimental features", "Pooled skim milk and whey samples were separated using 2-DE and gel spots that were altered in abundance were excised, digested with trypsin and identified by MALDI-TOF. The pooled samples were also analysed by 1D electrophoresis, slices of each lane were subjected to trypsin digestion, and the extracted peptides were analysed by LC-MS/MS. Peptide abundance was estimated by peptide count and EMPAI value. The pooled samples were also digested, isotope labelled and subjected to LC-MS/MS. Isotope ratios were determined for each peptide."], ["Data source location", "Waikato region of New Zealand"], ["Data accessibility", "Data is available within this article and through ProteomeXchange accession numbers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011."]], "row_count": 9, "column_count": 2, "numeric_cells": 3, "header_rows": 0, "locator": "section[1]/table-wrap[1]#t0005"}}], "gaps": []}, "analysis": {"state": "absent", "methods": [], "targets": [], "gaps": ["method", "operations"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "data", "blocked_on": "data:accession_absent", "axes": {"method": {"tier": "R4", "expressible": false, "required_operations": [], "missing_operations": [], "basis": "The reader quoted the method the paper names (not named), but the corpus's method extractor could not map it onto a DSL operation, so the method is published but not expressible."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R4", "reachable": false, "accessions": ["ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011"], "resolved_accessions": [], "statements": ["Data is available within this article and through ProteomeXchange accession numbers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011."], "basis": "The paper names ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011, but the accession does not resolve in the repository (no reason recorded), so the data cannot be reached from here. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:27274532", "asset:table:pmid:27274532:1"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:None"]}, "gaps": ["data:accession_absent"]}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:28:33Z", "independence": "same_provider"}, "evidence_check": {"method": null, "availability": "verbatim"}, "calls": 8, "usage": {"prompt_tokens": 22725, "completion_tokens": 2738, "cached_tokens": 2080}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:27274532", "type": "paper", "label": "pmid:27274532"}, {"id": "source:pmid:27274532", "type": "manuscript", "label": "PMID 27274532 full text"}, {"id": "data:geo:None", "type": "dataset", "label": "GEO None"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:27274532", "type": "paper", "label": "pmid:27274532"}, {"id": "source:pmid:27274532", "type": "manuscript", "label": "PMID 27274532 full text"}, {"id": "data:geo:None", "type": "dataset", "label": "GEO None"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-27274532", "type": "extraction", "used": ["source:pmid:27274532"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-None", "type": "accession_resolution", "used": ["data:geo:None"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:27274532", "parent_hash": "sha256:9867f9c91d40dabbcaf0c64bb13e919526f96b62a58ffb1254314ffda493d911", "fork_of": null, "generation": 2}, "gaps": ["execution.not_run", "method", "operations", "review.human_critique", "review.independent_reader", "review.machine_critique", "step:obtain_source_data blocked on data:accession_absent"], "assays": [{"role": "data_generation", "definition_id": "mass_spectrometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Proteomics data in support of the quantification of the", "Here we provide data from three proteomics techniques; two-dimensional electrophoresis", "-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl i"], "order_basis": "paper_text_order:0", "id": "assay:1"}]}