Data on the identification of protein interactors with the Evening Complex and PCH1 in Arabidopsis using tandem affinity purification and mass spectrometry (TAP-MS).

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Here we describe two datasets generated by a series of TAP-MS analyses to co-purify proteins associated with either ELF3 or ELF4 of the Evening Complex (EC) (\"Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry\" (Huang et al., 2016a) [1]) or proteins associated with PCH1, which is a newly identified output of the circadian clock to regulate photoperiodic growth in Arabidopsis thaliana (\"PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis\" (Huang et al. 2016b) [2]). We used either ELF3, ELF4 or PCH1 fused to a C-terminal tandem affinity tag (6xHis-3xFLAG) as baits and conducted purifications in various genetic mutant backgrounds. These data are discussed in recent publications [1,2], and are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002606 (for EC) and PRIDE: PXD003352 (for PCH1).", "claims": [{"id": "claim-data-access", "statement": "Data are within this article and have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002606 athttp://www.ebi.ac.uk/pride/archive/projects/PXD002606or dataset identifier PRIDE: PXD003352 athttp://www.ebi.ac.uk/pride/archive/projects/PXD003352", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27274533", "locator": "sections[1] \"p\" paragraph 1", "quote": "Data are within this article and have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002606 athttp://www.ebi.ac.uk/pride/archive/projects/PXD002606or dataset identifier PRIDE: PXD003352 athttp://www.ebi.ac.uk/pride/archive/projects/PXD003352"}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper describes two TAP–MS datasets used to identify proteins associated with ELF3, ELF4, or PCH1 and deposits them in PRIDE; no statistical method or numerical result for the registered target is reported.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27274533", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Here we describe two datasets generated by a series of TAP–MS analyses to co-purify proteins associated with either ELF3 or ELF4 of the Evening Complex (EC) (“Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry” (Huang et al., 2016a) [1]) or proteins associated with PCH1, which is a newly identified output of the circadian clock to regulate photoperiodic growth in Arabidopsis thaliana (“PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth inArabidopsis” (Huang et al. 2016b) [2])."}, {"asset": "source:pmid:27274533", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These data are discussed in recent publications [1,2], and are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002606 (for EC) and PRIDE: PXD003352 (for PCH1)."}, {"asset": "source:pmid:27274533", "locator": "sections[9] \"Data analysis\" paragraph 2", "quote": "Proteome Discoverer (ThermoFisher Scientific; v.1.4) was used to extract the peaks and generate the mgf files which were submitted to Mascot (Matrix Science, London, UK; v.2.5.0) for database search."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The paper describes two TAP–MS datasets used to identify proteins associated with ELF3, ELF4, or PCH1 and deposits them in PRIDE; no statistical method or numerical result for the registered target is reported.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27274533"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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