Data on differentially expressed microRNAs in the liver between nonalcoholic fatty liver disease and normal Wistar rat using Solexa sequencing.

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The data collected include small RNAs and microRNAs in liver tissue from high glucose-induced NAFLD Wistar rats, using normal Wistar rats as their negative controls. 6 small RNA libraries were constructed and the expression profiles were compared between the two groups. Here we describe in detail how the data, deposited in the Gene Expression Omnibus (GEO) with the accession number GEO: GSE68411, was generated including the basic analysis.", "claims": [{"id": "claim-method", "statement": "Significant differences were examined using one-way ANOVA and the Student׳s t test, and differences with P<0.05 were considered statistically significant for all experiments.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27331102", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Significant differences were examined using one-way ANOVA and the Student׳s t test, and differences with P<0.05 were considered statistically significant for all experiments."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are provided in the paper", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27331102", "locator": "sections[1] \"p\" paragraph 1", "quote": "Data are provided in the paper"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27331102"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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The livers of NAFLD group, chosen from among the samples with higher levels of hepatic triglycerides, and those from normal group, chosen randomly, were subjected to Solexa sequencing."], ["Sample source location", "Experimental Animal Center of Shandong University, Jinan, China."], ["Data accessibility", "Data are provided in the paper"]], "row_count": 9, "column_count": 2, "numeric_cells": 2, "header_rows": 0, "locator": "section[1]/table-wrap[1]#t0005"}}, {"id": "asset:table:pmid:27331102:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:e298e8bed2e12551686dc74f20003dff75a88af8459702b3b132108895640f1b", "bytes": 1088, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[1]/table-wrap[1]#t0005", "version": null, "note": null, "structure": {"pmcid": "PMC4906032", "section_index": 2, "section_title": "p", "row_count": 9, "column_count": 2}, "table": {"label": null, "caption": null, "rows": [["Subject area", "Biology"], ["More specific subject area", "Animal nutrition and metabolism"], ["Type of data", "Figure and Table"], ["How data was acquired", "Solexa sequencing of Illumina HiSeq 2000 Genome Analyzer and Quantitative PCR analysis was performed"], ["Data format", "Filtered and analyzed"], ["Experimental factors", "3 NAFLD rat liver tissue samples and 3 normal rat liver tissue samples were used for RNA sequencing."], ["Experimental features", "Rat model of NAFLD were successfully established and three biological repetitions were performed to reduce the variance caused by individual differences. 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