Dataset of integrin-linked kinase protein: Protein interactions in cardiomyocytes identified by mass spectrometry.

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Integrin linked kinase complexes were captured from mouse heart lysates using a commercial antibody, with subsequent liquid chromatography tandem mass spectral analysis. Interacting partners were identified using the MASCOT server, and important interactions verified using reverse immunoprecipitation and mass spectrometry. All ILK interacting proteins were identified in a non-biased manner, and are stored in the ProteomeXchange Consortium via the PRIDE partner repository (reference ID PRIDE: PXD001053). The functional role of identified ILK interactions in cardiomyocyte function and arrhythmia were subsequently confirmed in human iPSC-cardiomyocytes.", "claims": [{"id": "claim-data-access", "statement": "The RAW data files generated from LC-MS-MS analysis of ILK immunoprecipitations have been provided, and are deposited in the ProteomeExchange (reference ID PRIDE: PXD001053).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27408918", "locator": "sections[5] \"Data\" paragraph 2", "quote": "The RAW data files generated from LC-MS-MS analysis of ILK immunoprecipitations have been provided, and are deposited in the ProteomeExchange (reference ID PRIDE: PXD001053)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27408918"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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