Significance of BMI1 and FSCN1 expression in colorectal cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27488323", "kind": "science-record", "revision": 7, "content_hash": "sha256:eb29ff4682f69797317e44acb45403d3b78a5edb6b438c58ca4eab917839f361", "created_at": "2026-11-01T15:40:19Z", "updated_at": "2026-11-01T21:20:36Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Significance of BMI1 and FSCN1 expression in colorectal cancer.", "doi": "10.4103/1319-3767.187602", "pmid": "27488323", "authors": ["Nehad M Alajez"], "journal": "Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association", "year": 2016, "abstract": "BACKGROUND/AIMS: Colorectal cancer (CRC) is the third most common type of cancer in terms of incidence and the fourth in cause of death world-wide, underscoring the need to identify novel biomarkers for early diagnosis, as well as improved disease stratification and treatment choices.\nPATIENTS AND METHODS: The Gene Expression Omnibus (GSE21510) and the Cancer Genome Atlas (TCGA) CRC datasets were utilized in the current study. GeneSpring 13.0 was used for normalization and analysis. The log-rank test was used to compare the outcome between expression groups.\nRESULT: Significant upregulation of BMI1 (2.3 FC, P = 3.7 × 10-18) and FSCN1 (1.3 FC,P = 4.7 × 10-3) was observed in CRC. High BMI1 expression was associated with reduced overall survival (OS) [Hazard ratio (HR), 1.87; 95% CI. 1.17-3.03; P= 0.009] and reduced disease-free survival (DFS) [HR, 162; 95% CI 1.01-2.63;P = 0.045]. Similarly, high expression of FSCN1 was associated with reduced OS (HR, 2.0; 95% CI, 1.24-3.2; P= 0.0044) and reduced DFS (HR, 1.60; 95% CI, 0.99-2.57;P = 0.055). Importantly, BMI1high/FSCN1high patients experienced the worst OS (HR, 3.17; 95% CI, 1.77-6.15; P= 0.0002) and DFS (HR, 2.34; 95% CI, 1.27-4.67,P = 0.0078). Using pathway analyses, tumors overexpressing BMI1 were enriched in zinc finger proteins and genes involved in DNA binding and regulation of transcription, whereas tumors expressing FSCN1 were enriched in genes involved in cell migration.\nCONCLUSION: Our data revealed poor OS and DFS in CRC patients overexpressing BMI1 or FSCN1 and suggest that these two markers in combination may represent superior prognostic marker to either one. Targeting BMI1 and FSCN1 may also provide potential therapeutic opportunity in CRC.", "claims": [{"id": "claim-method", "statement": "Survival curve comparison was conducted using the log-rank (Mantel–Cox) test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27488323", "locator": "sections[6] \"Statistical analysis\" paragraph 2", "quote": "Survival curve comparison was conducted using the log-rank (Mantel–Cox) test."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using the GSE21510 Gene Expression Omnibus and TCGA colorectal cancer datasets, with the log-rank test comparing outcomes between expression groups, the study found that BMI1high/FSCN1high patients had the worst overall and disease-free survival.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27488323"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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